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AB313679

Human CrkL ELISA Kit

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Human CrkL ELISA Kit is a Sandwich ELISA for the measurement of Human CrkL in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

Crk-like protein, CRKL

1 Images
Sandwich ELISA - Human CrkL ELISA Kit (AB313679)
  • sELISA

Supplier Data

Sandwich ELISA - Human CrkL ELISA Kit (AB313679)

Example of standard curve using ab313679. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.21 ng/mL

Range

0.21 - 50 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB32018

Anti-CrkL antibody [Y244]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CrkL antibody [Y244] (AB32018)

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Proteins & Peptides

AB119733

Recombinant Human CrkL protein

SDS-PAGE - Recombinant Human CrkL protein (AB119733)

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  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human CrkL ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CrkL in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human CrkL coated on a 96-well plate.

This ELISA kit shows no cross-reactivity with the following cytokines tested: human Coagulation Factor X, CHMP2B, Contactin-3, Cortactin, Cyr61/CCN1, DAPP1, Dynactin Subunit 1/DCTN1, DFF45/ICAD, DRAK1, GRAP2, GRK5, Hydroxyacid Oxidase-1/HAO-1,
LRIG1, MMP-12, NCK1, Nectin-2/CD112, Nesfatin-1/Nucleobindin-2, Neurogranin, Nrf2, NUDT5, Nucleoporin NUP85, PAR1, Pancreatic Polypeptide/PP, Peroxiredoxin 2, PSMA1, PU.1/Spi-1, RalA, RCOR1/CoREST, Serpin B8, SH2D1A, SHP-1, Siglec-6/CD327, SorCS3, THAP11, ULBP-4/RAET1E, UNC5H3, VAMP-1, VAMP-2, PBEF/Visfatin.

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Recovery

[ { "sample": "Serum", "range": "81 - 91 %", "average": "= 87.61" }, { "sample": "Plasma", "range": "79 - 92 %", "average": "= 86.15" }, { "sample": "Cell culture media", "range": "81 - 99 %", "average": "= 90.42" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab313679-1x96Tests", "assetComponentDetails": [ { "size":"2 x 1 Vial", "name":"Biotinylated Human CrkL Detection Antibody", "number":"AB313679-CMP10", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB313679-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human CrkL Antibody-coated ELISA Plate", "number":"AB313679-CMP09", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin", "number":"AB313679-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB313679-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D (Item K)", "number":"AB313679-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human CrkL Protein Standard", "number":"AB313679-CMP11", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB313679-CMP07", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B (Item E)", "number":"AB313679-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

May mediate the transduction of intracellular signals.
See full target information CRKL
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com