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Human CrkL ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human CrkL in Human in Cell culture supernatant, Plasma, Serum samples.

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Images

Sandwich ELISA - Human CrkL ELISA Kit (AB313679), expandable thumbnail

Key facts

Detection method
Colorimetric
Sample types
Cell culture supernatant, Plasma, Serum
Assay type
Sandwich (quantitative)
Reactive species
Human
Range
0.21 - 50 ng/mL
Sensitivity
= 0.21 ng/mL

Reactivity data

Application
sELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Target data

Function

May mediate the transduction of intracellular signals.

Alternative names

What's included?

1 x 96 Tests
Components
200X HRP-Streptavidin
1 x 200 µL
20X Wash Buffer
1 x 25 mL
5X Assay Diluent B (Item E)
1 x 15 mL
5X Assay Diluent D (Item K)
1 x 15 mL
Biotinylated Human CrkL Detection Antibody
2 x 1 Vial
Human CrkL Antibody-coated ELISA Plate
1 x 1 Unit
Lyophilized Human CrkL Protein Standard
2 x 1 Vial
Stop Solution; 0.2M Sulfuric Acid
1 x 8 mL
TMB One-Step Substrate Reagent
1 x 12 mL

Recommended products

Human CrkL ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human CrkL in Human in Cell culture supernatant, Plasma, Serum samples.

Key facts

Detection method
Colorimetric
Sample types
Cell culture supernatant, Plasma, Serum
Assay type
Sandwich (quantitative)
Reactive species
Human
Range
0.21 - 50 ng/mL
Assay Platform
Pre-coated microplate (12 x 8 well strips)
Sensitivity
= 0.21 ng/mL

Recovery

Sample specific recovery

Sample type
Serum
Average %
= 87.61
Range
81 - 91 %
Sample type
Plasma
Average %
= 86.15
Range
79 - 92 %
Sample type
Cell culture media
Average %
= 90.42
Range
81 - 99 %

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

Human CrkL ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CrkL in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human CrkL coated on a 96-well plate.

This ELISA kit shows no cross-reactivity with the following cytokines tested: human Coagulation Factor X, CHMP2B, Contactin-3, Cortactin, Cyr61/CCN1, DAPP1, Dynactin Subunit 1/DCTN1, DFF45/ICAD, DRAK1, GRAP2, GRK5, Hydroxyacid Oxidase-1/HAO-1,
LRIG1, MMP-12, NCK1, Nectin-2/CD112, Nesfatin-1/Nucleobindin-2, Neurogranin, Nrf2, NUDT5, Nucleoporin NUP85, PAR1, Pancreatic Polypeptide/PP, Peroxiredoxin 2, PSMA1, PU.1/Spi-1, RalA, RCOR1/CoREST, Serpin B8, SH2D1A, SHP-1, Siglec-6/CD327, SorCS3, THAP11, ULBP-4/RAET1E, UNC5H3, VAMP-1, VAMP-2, PBEF/Visfatin.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Sandwich ELISA - Human CrkL ELISA Kit (ab313679), expandable thumbnail

    Sandwich ELISA - Human CrkL ELISA Kit (ab313679)

    Example of standard curve using ab313679. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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