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AB260058

Human CRP ELISA Kit (C-Reactive protein)

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(27 Publications)

Human CRP ELISA Kit (C-Reactive protein) is a single-wash 90-min Simplestep used to quantify Human CRP (C-Reactive protein) with a sensitivity of 5.36 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

PTX1, CRP, C-reactive protein

11 Images
ELISA - Human CRP ELISA Kit (C-Reactive protein) (AB260058)
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Supplier Data

ELISA - Human CRP ELISA Kit (C-Reactive protein) (AB260058)
Sandwich ELISA - Human CRP ELISA Kit (C-Reactive protein) (AB260058)
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Supplier Data

Sandwich ELISA - Human CRP ELISA Kit (C-Reactive protein) (AB260058)

Human CRP ELISA kit comparison.

Standard Curve comparison between ab260058 and ab181416 SimpleStep ELISA kits. ab260058 SimpleStep ELISA® kit shows comparable sensitivity.

Sandwich ELISA - Human CRP ELISA Kit (C-Reactive protein) (AB260058)
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Supplier Data

Sandwich ELISA - Human CRP ELISA Kit (C-Reactive protein) (AB260058)

Example of human CRP standard curve in Sample Diluent NS.

The CRP standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human CRP ELISA Kit (C-Reactive protein) (AB260058)
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Supplier Data

Sandwich ELISA - Human CRP ELISA Kit (C-Reactive protein) (AB260058)

Three concentrations of CRP recombinant protein were spiked in duplicate to the indicated biological matrix to evaluate signal recovery in the working range of the assay.

Sandwich ELISA - Human CRP ELISA Kit (C-Reactive protein) (AB260058)
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Sandwich ELISA - Human CRP ELISA Kit (C-Reactive protein) (AB260058)

Linearity of dilution.

Recombinant CRP was spiked into the following biological samples and diluted in a 2-fold dilution series in Sample Diluent NS.

Sandwich ELISA - Human CRP ELISA Kit (C-Reactive protein) (AB260058)
  • sELISA

Supplier Data

Sandwich ELISA - Human CRP ELISA Kit (C-Reactive protein) (AB260058)

Example of human CRP standard curve in Sample Diluent NS. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human CRP ELISA Kit (C-Reactive protein) (AB260058)
  • sELISA

Supplier Data

Sandwich ELISA - Human CRP ELISA Kit (C-Reactive protein) (AB260058)

Human CRP standard curve comparison.

Standard Curve comparison between human CRP SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

Sandwich ELISA - Human CRP ELISA Kit (C-Reactive protein) (AB260058)
  • sELISA

Supplier Data

Sandwich ELISA - Human CRP ELISA Kit (C-Reactive protein) (AB260058)

Serum from ten individual healthy human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CRP concentration was determined to be 535.7 ng/mL with a range of 28.1 – 1180.4 ng/mL.

Sandwich ELISA - Human CRP ELISA Kit (C-Reactive protein) (AB260058)
  • sELISA

Supplier Data

Sandwich ELISA - Human CRP ELISA Kit (C-Reactive protein) (AB260058)

Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

Native CRP was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human CRP ELISA Kit (C-Reactive protein) (AB260058)
  • sELISA

Supplier Data

Sandwich ELISA - Human CRP ELISA Kit (C-Reactive protein) (AB260058)

Interpolated concentrations of native CRP in human serum and plasma samples.

The concentrations of CRP were measured in duplicates, interpolated from the CRP standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 1000, plasma (citrate) 1 : 2000, plasma (EDTA) 1 : 1000, plasma (heparin) 1 : 1000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CRP concentration was determined to be 1025.5 ng/mL in serum, 1676.5 ng/mL in plasma (citrate) and 668.3 ng/mL in plasma (EDTA), and, 1062.8 ng/mL in plasma (heparin).

Sandwich ELISA - Human CRP ELISA Kit (C-Reactive protein) (AB260058)
  • sELISA

Supplier Data

Sandwich ELISA - Human CRP ELISA Kit (C-Reactive protein) (AB260058)

Interpolated concentrations of human CRP in cerebrospinal fluid.

Interpolated concentration of native CRP was measured in duplicate at different sample concentrations. Undiluted samples are 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 5.36 pg/mL

Range

18.75 - 1200 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human CRP ELISA Kit (C-Reactive protein) ab260058 is a rapid single-wash 90-min Sandwich ELISA to measure Human CRP (C-Reactive protein)in cell culture supernatant, CSF, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 5.36 pg/mL.

How the assay works

Human CRP (C-Reactive protein)SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human CRP ELISA Kit (C-Reactive protein) ab260058 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

C-reactive protein (CRP) displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. CRP can interact with DNA and histones and it may scavenge nuclear material released from damaged circulating cells. CRP is secreted; it forms a homopentamer pentaxin (or pentraxin) which have a discoid arrangement of 5 non-covalently bound subunits. CRP binds 2 calcium ions per subunit. The concentration of CRP in plasma increases greatly during acute phase response to tissue injury, infection or other inflammatory stimuli. It is induced by IL1/interleukin-1 and IL6//interleukin-6. This kit requires the use of acid treatment for the disassociation of CRP.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.4" } ]
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Recovery

[ { "sample": "Cerebral Spinal Fluid", "range": "80 - 101 %", "average": "= 88" }, { "sample": "Cell culture supernatant", "range": "99 - 100 %", "average": "= 100" }, { "sample": "Serum", "range": "105 - 112 %", "average": "= 109" }, { "sample": "EDTA Plasma", "range": "107 - 113 %", "average": "= 110" }, { "sample": "Heparin Plasma", "range": "112 - 119 %", "average": "= 114" }, { "sample": "Citrate plasma", "range": "105 - 109 %", "average": "= 107" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C-reactive protein (CRP) also known as CRP protein is an acute-phase protein with a molecular mass of approximately 25 kDa. It is primarily synthesized in the liver in response to cytokines. This protein appears in the blood where it binds to phosphocholine expressed on the surface of dead or dying cells. CRP is often used as a biomarker for inflammation in the body and its levels in human and canine (anti CRP used) blood samples are measured using assays like the CRP ELISA test or CRP ELISA kit.
Biological function summary

CRP plays a significant role in the innate immune system by recognizing foreign pathogens and damaged cells. It does not form part of a larger complex but itself can bind to specific ligands initiating the complement cascade. This activation enhances phagocytosis by macrophages contributing to the clearance of necrotic and apoptotic cells. The CRP protein therefore acts as a fast responder in the presence of inflammation making it a useful indicator for monitoring inflammatory diseases.

Pathways

CRP operates predominantly within the acute-phase response pathway. Its production is upregulated by cytokines such as IL-6 which connects it to inflammation-signaling pathways. CRP also interacts with the complement system bridging innate and adaptive immunity. Besides CRP could indirectly influence pathways involving other inflammatory proteins like serum amyloid A and haptoglobin creating a network of responses important for managing inflammation.

Elevated CRP levels are commonly associated with cardiovascular diseases and rheumatoid arthritis. In cardiovascular diseases CRP acts as a marker for atherosclerosis where the protein levels correlate with disease severity. It connects with other proteins involved in inflammatory processes such as fibrinogen to contribute to disease pathology. In rheumatoid arthritis CRP provides insights into disease activity helping in the assessment of treatment responses.
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Product protocols

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Target data

Displays several functions associated with host defense : it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
See full target information CRP
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Publications (27)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in medicine 12:1524198 PubMed40046929

2025

The impact of allergic contact dermatitis on the inflammatory response and repair in wound healing process.

Applications

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Species

Unspecified reactive species

Wei Zhang,Jiabao Xu,Songyan Qu,Hui Peng

International journal for equity in health 23:200 PubMed39369256

2024

Biological and contextual determinants of early development in marginalized Roma communities: A research protocol of the RomaREACH study.

Applications

Unspecified application

Species

Unspecified reactive species

Shoshana Chovan,Daniela Fiľakovská Bobáková,Andrea Madarasová Gecková,Beáta Hubková,Gabriela Štrkolcová,Sijmen A Reijneveld,Marlou L A de Kroon

Frontiers in microbiology 15:1407555 PubMed39184030

2024

Bariatric-induced microbiome changes alter MASLD development in association with changes in the innate immune system.

Applications

Unspecified application

Species

Unspecified reactive species

Simer Shera,William Katzka,Julianne C Yang,Candace Chang,Nerea Arias-Jayo,Venu Lagishetty,Anna Balioukova,Yijun Chen,Erik Dutson,Zhaoping Li,Emeran A Mayer,Joseph R Pisegna,Claudia Sanmiguel,Shrey Pawar,David Zhang,Madelaine Leitman,Laura Hernandez,Jonathan P Jacobs,Tien S Dong

Journal of inflammation (London, England) 21:30 PubMed39135051

2024

Evidence of aberrant anti-epstein-barr virus antibody response, though no viral reactivation, in people with post-stroke fatigue.

Applications

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Species

Unspecified reactive species

Isobel C Mouat,Li Zhu,Alperen Aslan,Barry W McColl,Stuart M Allan,Craig J Smith,Marion S Buckwalter,Laura McCulloch

PloS one 19:e0306798 PubMed39121088

2024

Lifestyle and mood correlates of cardiometabolic risk in people with serious mental illness on second-generation antipsychotic medications.

Applications

Unspecified application

Species

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Susanne U Miedlich,Priya Sahay,Telva E Olivares,J Steven Lamberti,Diane S Morse,Kevin P Brazill,Kavaljit H Chhabra,Lauren Bainbridge

BMC pulmonary medicine 24:354 PubMed39039507

2024

Increasing serum miR-223-3p indicates the onset, severe development, and adverse prognosis of bronchiectasis: a retrospective study.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Fang,Yao Xu,Chenghui Lin,Jiewen Yang,Dongxu Zhai,Qingyuan Zhuang,Wangli Qiu,Yun Wang,Longjuan Zhang

International journal of health sciences 18:46-57 PubMed38974651

2024

Synergy between psychological impact and biochemical manifestation of stress among the COVID-19 pandemic-affected population.

Applications

Unspecified application

Species

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Yusuf Saleem Khan,Abdullah Alsrhani,Salma Kaneez,Mahvish Fatima,Khalid Iqbal,Muharib Alruwaili,Aisha Farhana

The Journal of international medical research 52:3000605241253733 PubMed38811356

2024

Effect of high-dose intravenous iron injection on hepatic function in a rat model of cirrhosis.

Applications

Unspecified application

Species

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Ji-Hye Kwon,RyungA Kang,Sangmin Maria Lee,Tae Soo Hahm,Hyun Sung Cho,Gayoung Jin,Justin Sangwook Ko

Journal of inflammation research 17:1577-1587 PubMed38495342

2024

The Impact of Laparoscopic Appendectomy and Open Appendectomy on B7-H3-Mediated Intrinsic Immune Response in Children with Acute Suppurative Appendicitis.

Applications

Unspecified application

Species

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Chunsheng Duan,Yuan Li,Jiansu Ma,Yanglin Song,Lixia Zhou

Clinical and translational science 16:2765-2778 PubMed37926919

2023

Neutrophil elastase activates the release of extracellular traps from COPD blood monocyte-derived macrophages.

Applications

Unspecified application

Species

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Shuo Zheng,Apparao B Kummarapurugu,Gamze B Bulut,Aamer Syed,Le Kang,Judith A Voynow
View all publications
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