Human CRP ELISA Kit (C-Reactive protein)

• ELISA

Supplier Data

ELISA - Human CRP ELISA Kit (C-Reactive protein) (AB260058)

• sELISA

Supplier Data

Sandwich ELISA - Human CRP ELISA Kit (C-Reactive protein) (AB260058)

Human CRP ELISA kit comparison.

Standard Curve comparison between ab260058 and ab181416 SimpleStep ELISA kits. ab260058 SimpleStep ELISA^® kit shows comparable sensitivity.

• sELISA

Supplier Data

Sandwich ELISA - Human CRP ELISA Kit (C-Reactive protein) (AB260058)

Example of human CRP standard curve in Sample Diluent NS.

The CRP standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

• sELISA

Supplier Data

Sandwich ELISA - Human CRP ELISA Kit (C-Reactive protein) (AB260058)

Three concentrations of CRP recombinant protein were spiked in duplicate to the indicated biological matrix to evaluate signal recovery in the working range of the assay.

• sELISA

Supplier Data

Sandwich ELISA - Human CRP ELISA Kit (C-Reactive protein) (AB260058)

Linearity of dilution.

Recombinant CRP was spiked into the following biological samples and diluted in a 2-fold dilution series in Sample Diluent NS.

• sELISA

Supplier Data

Sandwich ELISA - Human CRP ELISA Kit (C-Reactive protein) (AB260058)

Example of human CRP standard curve in Sample Diluent NS. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

• sELISA

Supplier Data

Sandwich ELISA - Human CRP ELISA Kit (C-Reactive protein) (AB260058)

Human CRP standard curve comparison.

Standard Curve comparison between human CRP SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

• sELISA

Supplier Data

Sandwich ELISA - Human CRP ELISA Kit (C-Reactive protein) (AB260058)

Serum from ten individual healthy human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CRP concentration was determined to be 535.7 ng/mL with a range of 28.1 – 1180.4 ng/mL.

• sELISA

Supplier Data

Sandwich ELISA - Human CRP ELISA Kit (C-Reactive protein) (AB260058)

Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

Native CRP was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.

• sELISA

Supplier Data

Sandwich ELISA - Human CRP ELISA Kit (C-Reactive protein) (AB260058)

Interpolated concentrations of native CRP in human serum and plasma samples.

The concentrations of CRP were measured in duplicates, interpolated from the CRP standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 1000, plasma (citrate) 1 : 2000, plasma (EDTA) 1 : 1000, plasma (heparin) 1 : 1000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CRP concentration was determined to be 1025.5 ng/mL in serum, 1676.5 ng/mL in plasma (citrate) and 668.3 ng/mL in plasma (EDTA), and, 1062.8 ng/mL in plasma (heparin).

• sELISA

Supplier Data

Sandwich ELISA - Human CRP ELISA Kit (C-Reactive protein) (AB260058)

Interpolated concentrations of human CRP in cerebrospinal fluid.

Interpolated concentration of native CRP was measured in duplicate at different sample concentrations. Undiluted samples are 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.