Human CRP ELISA Kit (C-Reactive protein) is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human CRP (C-Reactive protein) in Cerebral Spinal Fluid, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.
Colorimetric
Cerebral Spinal Fluid, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
Sandwich (quantitative)
Human
18.75 - 1200 pg/mL
1h 30m
= 5.36 pg/mL
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
PTX1, CRP, C-reactive protein
Human CRP ELISA Kit (C-Reactive protein) is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human CRP (C-Reactive protein) in Cerebral Spinal Fluid, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.
Colorimetric
Cerebral Spinal Fluid, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
Sandwich (quantitative)
Human
18.75 - 1200 pg/mL
1h 30m
Pre-coated microplate (12 x 8 well strips)
= 5.36 pg/mL
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 1.4 |
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 4 |
Sample type | Average % | Range |
---|---|---|
Sample type Cerebral Spinal Fluid | Average % = 88 | Range 80 - 101 % |
Sample type Cell culture supernatant | Average % = 100 | Range 99 - 100 % |
Sample type Serum | Average % = 109 | Range 105 - 112 % |
Sample type EDTA Plasma | Average % = 110 | Range 107 - 113 % |
Sample type Heparin Plasma | Average % = 114 | Range 112 - 119 % |
Sample type Citrate plasma | Average % = 107 | Range 105 - 109 % |
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Human CRP ELISA Kit (C-Reactive protein) (ab260058) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CRP (C-Reactive protein) protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CRP (C-Reactive protein) with 5.36 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
ASSAY SPECIFICITY: This kit recognizes both native and recombinant human and monkey CRP protein in serum, plasma, and cell culture supernatant.
Cell and tissue extract, urine, milk, and saliva samples have not been tested with this kit.
CROSS REACTIVITY: Recombinant mouse CRP and rat CRP were both prepared at 50 ng/mL respectively and assayed for cross reactivity. No cross-reactivity was observed.
INTERFERENCE: Recombinant mouse CRP and rat CRP were both prepared at 50 ng/mL respectively and tested for interference. No interference with was observed.
SPECIES REACTIVITY: This kit recognizes human and monkey CRP protein.
Other species reactivity was determined by measuring 1:1000 serum samples of various species, interpolating the protein concentrations from the human standard curve, and expressing the interpolated concentrations as a percentage of the protein concentration in human serum assayed at the same dilution.
Reactivity < 3% was determined for the following species: Mouse, Rat, Cow.
Other species reactivity not determined.
C-reactive protein (CRP) displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. CRP can interact with DNA and histones and it may scavenge nuclear material released from damaged circulating cells. CRP is secreted; it forms a homopentamer pentaxin (or pentraxin) which have a discoid arrangement of 5 non-covalently bound subunits. CRP binds 2 calcium ions per subunit. The concentration of CRP in plasma increases greatly during acute phase response to tissue injury, infection or other inflammatory stimuli. It is induced by IL1/interleukin-1 and IL6//interleukin-6. This kit requires the use of acid treatment for the disassociation of CRP.
This supplementary information is collated from multiple sources and compiled automatically.
C-reactive protein (CRP) also known as CRP protein is an acute-phase protein with a molecular mass of approximately 25 kDa. It is primarily synthesized in the liver in response to cytokines. This protein appears in the blood where it binds to phosphocholine expressed on the surface of dead or dying cells. CRP is often used as a biomarker for inflammation in the body and its levels in human and canine (anti CRP used) blood samples are measured using assays like the CRP ELISA test or CRP ELISA kit.
CRP plays a significant role in the innate immune system by recognizing foreign pathogens and damaged cells. It does not form part of a larger complex but itself can bind to specific ligands initiating the complement cascade. This activation enhances phagocytosis by macrophages contributing to the clearance of necrotic and apoptotic cells. The CRP protein therefore acts as a fast responder in the presence of inflammation making it a useful indicator for monitoring inflammatory diseases.
CRP operates predominantly within the acute-phase response pathway. Its production is upregulated by cytokines such as IL-6 which connects it to inflammation-signaling pathways. CRP also interacts with the complement system bridging innate and adaptive immunity. Besides CRP could indirectly influence pathways involving other inflammatory proteins like serum amyloid A and haptoglobin creating a network of responses important for managing inflammation.
Elevated CRP levels are commonly associated with cardiovascular diseases and rheumatoid arthritis. In cardiovascular diseases CRP acts as a marker for atherosclerosis where the protein levels correlate with disease severity. It connects with other proteins involved in inflammatory processes such as fibrinogen to contribute to disease pathology. In rheumatoid arthritis CRP provides insights into disease activity helping in the assessment of treatment responses.
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Human CRP standard curve comparison.
Standard Curve comparison between human CRP SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
Human CRP ELISA kit comparison comparison.
Standard Curve comparison between ab260058 and ab181416 SimpleStep ELISA kits. ab260058 SimpleStep ELISA kit shows comparable sensitivity.
Example of human CRP standard curve in Sample Diluent NS. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Interpolated concentrations of native CRP in human serum and plasma samples.
The concentrations of CRP were measured in duplicates, interpolated from the CRP standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:1000, plasma (citrate) 1:2000, plasma (EDTA) 1:1000, plasma (heparin) 1:1000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CRP concentration was determined to be 1025.5 ng/mL in serum, 1676.5 ng/mL in plasma (citrate) and 668.3 ng/mL in plasma (EDTA), and, 1062.8 ng/mL in plasma (heparin).
Serum from ten individual healthy human male donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CRP concentration was determined to be 535.7 ng/mL with a range of 28.1 – 1180.4 ng/mL.
Example of human CRP standard curve in Sample Diluent NS.
The CRP standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.
Native CRP was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.
Linearity of dilution.
Recombinant CRP was spiked into the following biological samples and diluted in a 2-fold dilution series in Sample Diluent NS.
Three concentrations of CRP recombinant protein were spiked in duplicate to the indicated biological matrix to evaluate signal recovery in the working range of the assay.
Interpolated concentrations of human CRP in cerebrospinal fluid.
Interpolated concentration of native CRP was measured in duplicate at different sample concentrations. Undiluted samples are 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
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