Human CRP ELISA Kit (C-Reactive protein), Fluorescent
- Recombinant
- SimpleStep
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Human CRP ELISA Kit (C-Reactive protein), Fluorescent is a single-wash 90-min Simplestep used to quantify Human CRP (C-Reactive protein) with a sensitivity of 1.23 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Fluorescent Sandwich ELISA - 530/570/590 nm readout : works on any standard plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)
View Alternative Names
PTX1, CRP, C-reactive protein
- sELISA
Unknown
Sandwich ELISA - Human CRP ELISA Kit (C-Reactive protein), Fluorescent (AB278042)
Example of human CRP standard curve in Sample Diluent NS.
Raw data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Human CRP ELISA Kit (C-Reactive protein), Fluorescent (AB278042)
Interpolated concentrations of native CRP in human serum and plasma samples.
The concentrations of CRP were measured in duplicates, interpolated from the CRP standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 1000, plasma (citrate) 1 : 2000, plasma (EDTA) 1 : 1000, plasma (heparin) 1 : 1000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CRP concentration was determined to be 1025.5 ng/mL in serum, 1676.5 ng/mL in plasma (citrate) and 668.3 ng/mL in plasma (EDTA), and, 1062.8 ng/mL in plasma (heparin).
- sELISA
Supplier Data
Sandwich ELISA - Human CRP ELISA Kit (C-Reactive protein), Fluorescent (AB278042)
Serum from ten individual healthy human male donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CRP concentration was determined to be 535.7 ng/mL with a range of 28.1 – 1180.4 ng/mL.
- sELISA
Supplier Data
Sandwich ELISA - Human CRP ELISA Kit (C-Reactive protein), Fluorescent (AB278042)
Interpolated concentrations of human CRP in cerebrospinal fluid.
Interpolated concentration of native CRP was measured in duplicate at different sample concentrations. Undiluted samples are 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Reactivity data
Product details
C-Reactive Protein (CRP) in vitro CatchPoint SimpleStep ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of C-Reactive Protein (CRP) protein in human serum, plasma, and cell culture supernatants.
This CatchPoint SimpleStep ELISA kit has been optimized for Molecular Devices Microplate Readers. Click here for a list of recommended Microplate Readers.
If using a Molecular Devices' plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint SimpleStep ELISA Kits is available with all the protocol and analysis settings at www.softmaxpro.org.
The CatchPoint SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPoint HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission.
CRP displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. CRP can interact with DNA and histones and it may scavenge nuclear material released from damaged circulating cells. CRP is secreted; it forms a homopentamer pentaxin (or pentraxin) which have a discoid arrangement of 5 non-covalently bound subunits. CRP binds 2 calcium ions per subunit. The concentration of CRP in plasma increases greatly during acute phase response to tissue injury, infection or other inflammatory stimuli. It is induced by IL1/interleukin-1 and IL6//interleukin-6.
Precision
Recovery
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
CRP plays a significant role in the innate immune system by recognizing foreign pathogens and damaged cells. It does not form part of a larger complex but itself can bind to specific ligands initiating the complement cascade. This activation enhances phagocytosis by macrophages contributing to the clearance of necrotic and apoptotic cells. The CRP protein therefore acts as a fast responder in the presence of inflammation making it a useful indicator for monitoring inflammatory diseases.
Pathways
CRP operates predominantly within the acute-phase response pathway. Its production is upregulated by cytokines such as IL-6 which connects it to inflammation-signaling pathways. CRP also interacts with the complement system bridging innate and adaptive immunity. Besides CRP could indirectly influence pathways involving other inflammatory proteins like serum amyloid A and haptoglobin creating a network of responses important for managing inflammation.
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