Human CSF-1-R ELISA Kit ab230940 is a single-wash 90-min SimpleStep ELISA used to quantify Human CSF-1-R with a sensitivity of 20.1 pg/ml. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.Easy-to-use, rapid, sensitive Human CSF-1-R sandwich ELISA Kit:- Mix, Wash, Read: get results in 90 minutes with SimpleStep ELISA® format- Colorimetric assay - 450nm readout; works on any plate reader- Looking for more flexibility? We also offer antibody pairs ready for conjugation
Colorimetric
Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
Sandwich (quantitative)
Human
78.1 - 5000 pg/mL
1h 30m
= 20.1 pg/mL
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Tyrosine-protein kinase that acts as a cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of pro-inflammatory chemokines in response to IL34 and CSF1, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone and tooth development. Required for normal male and female fertility, and for normal development of milk ducts and acinar structures in the mammary gland during pregnancy. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration, and promotes cancer cell invasion. Activates several signaling pathways in response to ligand binding, including the ERK1/2 and the JNK pathway (PubMed:20504948, PubMed:30982609). Phosphorylates PIK3R1, PLCG2, GRB2, SLA2 and CBL. Activation of PLCG2 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, that then lead to the activation of protein kinase C family members, especially PRKCD. Phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leads to activation of the AKT1 signaling pathway. Activated CSF1R also mediates activation of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1, and of the SRC family kinases SRC, FYN and YES1. Activated CSF1R transmits signals both via proteins that directly interact with phosphorylated tyrosine residues in its intracellular domain, or via adapter proteins, such as GRB2. Promotes activation of STAT family members STAT3, STAT5A and/or STAT5B. Promotes tyrosine phosphorylation of SHC1 and INPP5D/SHIP-1. Receptor signaling is down-regulated by protein phosphatases, such as INPP5D/SHIP-1, that dephosphorylate the receptor and its downstream effectors, and by rapid internalization of the activated receptor. In the central nervous system, may play a role in the development of microglia macrophages (PubMed:30982608).
FMS, CSF1R, Macrophage colony-stimulating factor 1 receptor, CSF-1 receptor, Proto-oncogene c-Fms, CSF-1-R, CSF-1R, M-CSF-R
Human CSF-1-R ELISA Kit ab230940 is a single-wash 90-min SimpleStep ELISA used to quantify Human CSF-1-R with a sensitivity of 20.1 pg/ml. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.Easy-to-use, rapid, sensitive Human CSF-1-R sandwich ELISA Kit:- Mix, Wash, Read: get results in 90 minutes with SimpleStep ELISA® format- Colorimetric assay - 450nm readout; works on any plate reader- Looking for more flexibility? We also offer antibody pairs ready for conjugation
Colorimetric
Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
Sandwich (quantitative)
Human
78.1 - 5000 pg/mL
1h 30m
Pre-coated microplate (12 x 8 well strips)
= 20.1 pg/mL
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Human Serum | n 9 | mean - | SD - | C.V. 6 |
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Human Serum | n 9 | mean - | SD - | C.V. 5.6 |
Sample type | Average % | Range |
---|---|---|
Sample type Serum | Average % = 105 | Range 104 - 107 % |
Sample type EDTA Plasma | Average % = 108 | Range 106 - 111 % |
Sample type Cell culture extracts | Average % = 102 | Range 101 - 103 % |
Sample type Heparin Plasma | Average % = 108 | Range 105 - 111 % |
Sample type Citrate plasma | Average % = 110 | Range 107 - 113 % |
Sample type Tissue | Average % = 108 | Range 106 - 110 % |
Sample type Tissue Culture Media | Average % = 101 | Range 101 - 102 % |
Blue Ice
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Human CSF-1-R ELISA Kit (ab230940) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CSF-1-R protein in edta plasma, hep plasma, serum, tissue extracts, cit plasma, and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CSF-1-R with 13.1 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Serum from ten individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CSF-1-R concentration was determined to be 141 ng/mL with a range of 81 – 240 ng/mL.
Replace. Interpolated concentrations of native CSF-1-R in human serum and plasma samples. The concentrations of CSF-1-R were measured in duplicates, interpolated from the CSF-1-R standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:400, plasma (heparin) 1:200, plasma (citrate) 1:400 and plasma (EDTA) 1:400. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CSF-1-R concentration was determined to be 163 ng/mL in neat serum, 113 ng/mL in neat plasma (heparin), 144 ng/mL in neat plasma (citrate), and 149 ng/mL in neat plasma (EDTA).
Interpolated concentrations of native CSF-1-R in human THP-1 cell extract, kidney extract, and liver extract. The concentrations of CSF-1-R were measured in duplicate and interpolated from the CSF-1-R standard curve and corrected for sample dilution. Undiluted samples are as follows: THP-1 cell extract 500 µg/mL, kidney extract 100 µg/mL, and liver extract 500 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CSF-1-R concentration in the undiluted samples was determined to be 2,100 pg/mL in THP-1 cell extract, 489 pg/mL in kidney extract, and 733 pg/mL in liver extract.
The CSF-1-R standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
The CSF-1-R standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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