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Human CSF-1-R ELISA Kit ab230940 is a single-wash 90-min SimpleStep ELISA used to quantify Human CSF-1-R with a sensitivity of 20.1 pg/ml. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.Easy-to-use, rapid, sensitive Human CSF-1-R sandwich ELISA Kit:- Mix, Wash, Read: get results in 90 minutes with SimpleStep ELISA® format- Colorimetric assay - 450nm readout; works on any plate reader- Looking for more flexibility? We also offer antibody pairs ready for conjugation

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Images

Sandwich ELISA - Human CSF-1-R ELISA Kit (AB230940), expandable thumbnail
  • Sandwich ELISA - Human CSF-1-R ELISA Kit (AB230940), expandable thumbnail
  • Sandwich ELISA - Human CSF-1-R ELISA Kit (AB230940), expandable thumbnail
  • Sandwich ELISA - Human CSF-1-R ELISA Kit (AB230940), expandable thumbnail
  • Sandwich ELISA - Human CSF-1-R ELISA Kit (AB230940), expandable thumbnail

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

78.1 - 5000 pg/mL

Assay time

1h 30m

Sensitivity

= 20.1 pg/mL

Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Tyrosine-protein kinase that acts as a cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of pro-inflammatory chemokines in response to IL34 and CSF1, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone and tooth development. Required for normal male and female fertility, and for normal development of milk ducts and acinar structures in the mammary gland during pregnancy. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration, and promotes cancer cell invasion. Activates several signaling pathways in response to ligand binding, including the ERK1/2 and the JNK pathway (PubMed:20504948, PubMed:30982609). Phosphorylates PIK3R1, PLCG2, GRB2, SLA2 and CBL. Activation of PLCG2 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, that then lead to the activation of protein kinase C family members, especially PRKCD. Phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leads to activation of the AKT1 signaling pathway. Activated CSF1R also mediates activation of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1, and of the SRC family kinases SRC, FYN and YES1. Activated CSF1R transmits signals both via proteins that directly interact with phosphorylated tyrosine residues in its intracellular domain, or via adapter proteins, such as GRB2. Promotes activation of STAT family members STAT3, STAT5A and/or STAT5B. Promotes tyrosine phosphorylation of SHC1 and INPP5D/SHIP-1. Receptor signaling is down-regulated by protein phosphatases, such as INPP5D/SHIP-1, that dephosphorylate the receptor and its downstream effectors, and by rapid internalization of the activated receptor. In the central nervous system, may play a role in the development of microglia macrophages (PubMed:30982608).

Alternative names

What's included?

1 x 96 Tests
Components
10X Human CSF-1-R Capture Antibody
1 x 600 µL
10X Human CSF-1-R Detector Antibody
1 x 600 µL
10X Wash Buffer PT (ab206977)
1 x 20 mL
50X Cell Extraction Enhancer Solution (ab193971)
1 x 1 mL
5X Cell Extraction Buffer PTR (ab193970)
1 x 10 mL
Antibody Diluent 4BR
1 x 6 mL
Human CSF-1-R Lyophilized Recombinant Protein
2 x 1 Vial
Plate Seals
1 x 1 Unit
Sample Diluent NS (ab193972)
1 x 50 mL
SimpleStep Pre-Coated 96-Well Microplate (ab206978)
1 x 1 Unit
Stop Solution
1 x 12 mL
TMB Development Solution
1 x 12 mL

Recommended products

Human CSF-1-R ELISA Kit ab230940 is a single-wash 90-min SimpleStep ELISA used to quantify Human CSF-1-R with a sensitivity of 20.1 pg/ml. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.Easy-to-use, rapid, sensitive Human CSF-1-R sandwich ELISA Kit:- Mix, Wash, Read: get results in 90 minutes with SimpleStep ELISA® format- Colorimetric assay - 450nm readout; works on any plate reader- Looking for more flexibility? We also offer antibody pairs ready for conjugation

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

78.1 - 5000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 20.1 pg/mL

Precision

Intra assay

Sample

Human Serum

n
9
mean
-
SD
-
C.V.

6

Inter assay

Sample

Human Serum

n
9
mean
-
SD
-
C.V.

5.6

Recovery

Sample specific recovery

Sample type

Serum

Average %

= 105

Range

104 - 107 %

Sample type

EDTA Plasma

Average %

= 108

Range

106 - 111 %

Sample type

Cell culture extracts

Average %

= 102

Range

101 - 103 %

Sample type

Heparin Plasma

Average %

= 108

Range

105 - 111 %

Sample type

Citrate plasma

Average %

= 110

Range

107 - 113 %

Sample type

Tissue

Average %

= 108

Range

106 - 110 %

Sample type

Tissue Culture Media

Average %

= 101

Range

101 - 102 %

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Human CSF-1-R ELISA Kit (ab230940) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CSF-1-R protein in edta plasma, hep plasma, serum, tissue extracts, cit plasma, and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CSF-1-R with 13.1 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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5 product images

  • Sandwich ELISA - Human CSF-1-R ELISA Kit (ab230940), expandable thumbnail

    Sandwich ELISA - Human CSF-1-R ELISA Kit (ab230940)

    Serum from ten individual healthy human female donors was measured in duplicate.

    Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CSF-1-R concentration was determined to be 141 ng/mL with a range of 81 – 240 ng/mL.

  • Sandwich ELISA - Human CSF-1-R ELISA Kit (ab230940), expandable thumbnail

    Sandwich ELISA - Human CSF-1-R ELISA Kit (ab230940)

    Replace. Interpolated concentrations of native CSF-1-R in human serum and plasma samples. The concentrations of CSF-1-R were measured in duplicates, interpolated from the CSF-1-R standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:400, plasma (heparin) 1:200, plasma (citrate) 1:400 and plasma (EDTA) 1:400. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CSF-1-R concentration was determined to be 163 ng/mL in neat serum, 113 ng/mL in neat plasma (heparin), 144 ng/mL in neat plasma (citrate), and 149 ng/mL in neat plasma (EDTA).

  • Sandwich ELISA - Human CSF-1-R ELISA Kit (ab230940), expandable thumbnail

    Sandwich ELISA - Human CSF-1-R ELISA Kit (ab230940)

    Interpolated concentrations of native CSF-1-R in human THP-1 cell extract, kidney extract, and liver extract. The concentrations of CSF-1-R were measured in duplicate and interpolated from the CSF-1-R standard curve and corrected for sample dilution. Undiluted samples are as follows: THP-1 cell extract 500 µg/mL, kidney extract 100 µg/mL, and liver extract 500 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CSF-1-R concentration in the undiluted samples was determined to be 2,100 pg/mL in THP-1 cell extract, 489 pg/mL in kidney extract, and 733 pg/mL in liver extract.

  • Sandwich ELISA - Human CSF-1-R ELISA Kit (ab230940), expandable thumbnail

    Sandwich ELISA - Human CSF-1-R ELISA Kit (ab230940)

    The CSF-1-R standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • Sandwich ELISA - Human CSF-1-R ELISA Kit (ab230940), expandable thumbnail

    Sandwich ELISA - Human CSF-1-R ELISA Kit (ab230940)

    The CSF-1-R standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

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Product protocols

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