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AB267807

Human CSPG4 ELISA Kit (NG2/MCSP)

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Human CSPG4 ELISA Kit (NG2/MCSP) is a single-wash 90-min Simplestep used to quantify Human CSPG4 (NG2/MCSP) with a sensitivity of 25.5 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

MCSP, CSPG4, Chondroitin sulfate proteoglycan 4, Chondroitin sulfate proteoglycan NG2, Melanoma chondroitin sulfate proteoglycan, Melanoma-associated chondroitin sulfate proteoglycan

2 Images
Sandwich ELISA - Human CSPG4 ELISA Kit (NG2/MCSP) (AB267807)
  • sELISA

Supplier Data

Sandwich ELISA - Human CSPG4 ELISA Kit (NG2/MCSP) (AB267807)

Interpolated concentrations of native CSPG4 in human serum and plasma

Sandwich ELISA - Human CSPG4 ELISA Kit (NG2/MCSP) (AB267807)
  • sELISA

Supplier Data

Sandwich ELISA - Human CSPG4 ELISA Kit (NG2/MCSP) (AB267807)

Example of human CSPG4 standard curve in Sample Diluent NS

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Citrate plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 25.5 pg/mL

Range

156.25 - 10000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human CSPG4 ELISA Kit (NG2/MCSP) (ab267807) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CSPG4 (NG2/MCSP) protein in serum, cit plasma, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CSPG4 (NG2/MCSP) with 25.5 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Chondroitin sulfate proteoglycan 4 (CSPG4), also known as NG2 or MCSP, is a multifunctional cell surface type I transmembrane protein that is covalently modified with chondroitin sulfate glycosaminoglycan. CSPG4 has been observed to promote epidermal stem cell patterned clustering and used as a cell surface marker for melanoma and glial precursor cells. Studies have associate CSPG4 as important role in facilitating the growth and survival of malignant melanoma as well as migration, invasion, metastasis, and chemoresistance.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "0.5" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.5" } ]
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Recovery

[ { "sample": "Serum", "range": "113 - 115 %", "average": "= 114" }, { "sample": "Cell culture media", "range": "100 - 101 %", "average": "= 100" }, { "sample": "Citrate plasma", "range": "103 - 109 %", "average": "= 106" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N-terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades.
See full target information CSPG4
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