Human CST6 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human CST6 in Human in Plasma, Cell culture supernatant, Serum samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.123 - 100 ng/mL
- Sensitivity
- = 0.14 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
High affinity inhibitor for cathepsin L, cathepsin L2 (cathepsin V), and legumain (PubMed:30425301). Involved in the regulation of epidermal cornification, and hair follicle morphogenesis and maintenance (PubMed:30425301).
Alternative names
Cystatin-M, Cystatin-6, Cystatin-E, CST6
What's included?
Recommended products
Human CST6 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human CST6 in Human in Plasma, Cell culture supernatant, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.123 - 100 ng/mL
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 0.14 ng/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 10 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 12 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 118.9 | Range 83 - 143 % |
Sample type Plasma | Average % = 103.6 | Range 72 - 141 % |
Sample type Cell culture media | Average % = 100.5 | Range 82 - 129 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Human CST6 ELISA Kit (ab277411) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CST6 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human CST6 coated on a 96-well plate. Standards and samples are pipetted into the wells and Human CST6 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human CST6 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human CST6 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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1 product image
ELISA - Human CST6 ELISA Kit (ab277411)
These standard curves are for demonstration only. A standard curve must be run with each assay.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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