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AB277411

Human CST6 ELISA Kit

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Human CST6 ELISA Kit is a Sandwich ELISA for the measurement of Human CST6 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

Cystatin-M, Cystatin-6, Cystatin-E, CST6

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ELISA - Human CST6 ELISA Kit (AB277411)
  • ELISA

Supplier Data

ELISA - Human CST6 ELISA Kit (AB277411)

These standard curves are for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.14 ng/mL

Range

0.123 - 100 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human CST6 ELISA Kit (ab277411) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CST6 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Human CST6 coated on a 96-well plate. Standards and samples are pipetted into the wells and Human CST6 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human CST6 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human CST6 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": "83 - 143 %", "average": "= 118.9" }, { "sample": "Plasma", "range": "72 - 141 %", "average": "= 103.6" }, { "sample": "Cell culture media", "range": "82 - 129 %", "average": "= 100.5" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab277411-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB277411-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human CST6 Antibody", "number":"AB277411-CMP09", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB277411-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human CST6 coated Microplate", "number":"AB277411-CMP01", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB277411-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human CST6 standard protein (Lyophilized)", "number":"AB277411-CMP06", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D", "number":"AB277411-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB277411-CMP07", "productcode":"" }, { "size":"1 x 200 µL", "name":"250X HRP-Streptavidin Concentrate", "number":"AB277411-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

High affinity inhibitor for cathepsin L, cathepsin L2 (cathepsin V), and legumain (PubMed : 30425301). Involved in the regulation of epidermal cornification, and hair follicle morphogenesis and maintenance (PubMed : 30425301).
See full target information CST6
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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