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AB216166

Human CT-1 ELISA Kit

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(1 Publication)

Human CT-1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CT-1 with a sensitivity of 3.2 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Cardiotrophin-1, CT-1, CTF1

7 Images
Sandwich ELISA - Human CT-1 ELISA Kit (AB216166)
  • sELISA

Supplier Data

Sandwich ELISA - Human CT-1 ELISA Kit (AB216166)

Interpolated concentrations of spike Cardiotrophin 1 in human serum and cell culture media (RPMI + 10% FBS) samples. concentrations of spike Cardiotrophin 1 in human plasma samples.

The concentrations of Cardiotrophin 1 were measured in duplicates, interpolated from the Cardiotrophin 1 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 100% and cell culture media 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Human CT-1 ELISA Kit (AB216166)
  • sELISA

Supplier Data

Sandwich ELISA - Human CT-1 ELISA Kit (AB216166)

Interpolated concentrations of spike Cardiotrophin 1 in human plasma samples.

The concentrations of Cardiotrophin 1 were measured in duplicates, interpolated from the Cardiotrophin 1 standard curves and corrected for sample dilution. Undiluted samples are as follows : plasma (citrate) 100%, plasma (heparin) 100%, and plasma (EDTA) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Human CT-1 ELISA Kit (AB216166)
  • sELISA

Supplier Data

Sandwich ELISA - Human CT-1 ELISA Kit (AB216166)

Other species reactivity was determined by measuring a 1,000 µg/mL extract load of various species heart tissue extract.

The protein concentrations were interpolated from the human Cardiotrophin standard curve.

Sandwich ELISA - Human CT-1 ELISA Kit (AB216166)
  • sELISA

Supplier Data

Sandwich ELISA - Human CT-1 ELISA Kit (AB216166)

Example of human Cardiotrophin 1 standard curve in Sample Diluent 25BS.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human CT-1 ELISA Kit (AB216166)
  • sELISA

Supplier Data

Sandwich ELISA - Human CT-1 ELISA Kit (AB216166)

Interpolated concentrations of native Cardiotrophin 1 in human heart, skeletal muscle, and liver based on a 1,000 μg/mL extract load.

The concentrations of Cardiotrophin 1 were measured in duplicate and interpolated from the Cardiotrophin 1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cardiotrophin 1 concentration was determined to be 1,029 pg/mL in heart, 1,550 pg/mL in skeletal muscle, and 78 pg/mL in liver.

Sandwich ELISA - Human CT-1 ELISA Kit (AB216166)
  • sELISA

Supplier Data

Sandwich ELISA - Human CT-1 ELISA Kit (AB216166)

Interpolated concentrations of native Cardiotrophin 1 in PC-3 and U-87 MG cell extracts based on a 1,000 μg/mL extract load.

The concentrations of Cardiotrophin 1 were measured in duplicate and interpolated from the Cardiotrophin 1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cardiotrophin 1 concentration was determined to be 429 pg/mL in PC-3, and 811 pg/mL in U-87 MG.

Sandwich ELISA - Human CT-1 ELISA Kit (AB216166)
  • sELISA

Supplier Data

Sandwich ELISA - Human CT-1 ELISA Kit (AB216166)

Example of human Cardiotrophin 1 standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 3.2 pg/mL

Range

23.44 - 1500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human CT-1 ELISA Kit (ab216166) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CT-1 protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CT-1 with 3.2 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

CT-1 (Cardiotrophin 1) is a secreted member of the IL-6 cytokine superfamily. It is a 201 amino acid protein which binds to and activates the ILST/gp130 heterodimer receptor. CT-1 induces cardiomyocyte hypertrophy in vitro and is a biomarker implicated in hypertension. It is highly expressed in heart, skeletal muscle, prostate and ovary. It has lower expression levels in lung, kidney, pancreas, thymus, testis and small intestine. There is little or no expression of CT-1 in brain, placenta, liver, spleen, colon or peripheral blood leukocytes.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.2" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.2" } ]
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Recovery

[ { "sample": "Serum", "range": "104 - 122 %", "average": "= 115" }, { "sample": "EDTA Plasma", "range": "106 - 116 %", "average": "= 109" }, { "sample": "Cell culture extracts", "range": "106 - 128 %", "average": "= 114" }, { "sample": "Tissue Extracts", "range": "102 - 121 %", "average": "= 109" }, { "sample": "Heparin Plasma", "range": "105 - 109 %", "average": "= 107" }, { "sample": "Citrate plasma", "range": "90 - 95 %", "average": "= 92" }, { "sample": "Cell culture media", "range": "106 - 108 %", "average": "= 107" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab216166-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB216166-CMP07", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB216166-CMP15", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB216166-CMP14", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB216166-CMP13", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human CT-1 Lyophilized Recombinant Protein", "number":"AB216166-CMP12", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample Diluent 25BS", "number":"AB216166-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CT-1 Capture Antibody", "number":"AB216166-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB216166-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CT-1 Detector Antibody", "number":"AB216166-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB216166-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB216166-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB216166-CMP08", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB216166-CMP09", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Induces cardiac myocyte hypertrophy in vitro. Binds to and activates the ILST/gp130 receptor.
See full target information CTF1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Mediators of inflammation 2023:6112301 PubMed37830075

2023

Empagliflozin Reverses Oxidized LDL-Induced RECK Suppression, Cardiotrophin-1 Expression, MMP Activation, and Human Aortic Smooth Muscle Cell Proliferation and Migration.

Applications

Unspecified application

Species

Unspecified reactive species

Bysani Chandrasekar,Srinivas Mummidi,Vincent G DeMarco,Yusuke Higashi
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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