Human CTGF ELISA kit is a single-wash 90-min Simplestep used to quantify Human CTGF with a sensitivity of 64 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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- Circulation journal : official journal of the Japanese Circulation Society 87:527-5352023Plasma Connective Tissue Growth Factor as a Biomarker of Pulmonary Arterial Hypertension Associated With Congenital Heart Disease in Adults.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenshi Liu,Ying Hua,Dongdong Zheng,Wenjing Lv,Wei Zhang,Qianqian Chen,Rong Huang,Xiaofei LiPubMed 36843115
- Aging 15:830-8452023The context-dependent role of transforming growth factor-β/miR-378a-3p/connective tissue growth factor in vascular calcification: a translational study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYou-Tien Tsai,Hsiang-Yuan Yeh,Chia-Ter Chao,Jenq-Wen Huang,Chih-Kang ChiangPubMed 36787443
- Life (Basel, Switzerland) 12:2022Data Mining Identifies and as Biomarker Candidates for Cardiac Hypertrophy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarkus Johansson,Benyapa Tangruksa,Sepideh Heydarkhan-Hagvall,Anders Jeppsson,Peter Sartipy,Jane SynnergrenPubMed 35629393
- Molecular therapy. Nucleic acids 28:831-8462022U1A is a positive regulator of the expression of heterologous and cellular genes involved in cell proliferation and migration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEric Rovira,Beatriz Moreno,Nerea Razquin,Roland Hjerpe,Monika Gonzalez-Lopez,Rosa Barrio,Igor Ruiz de Los Mozos,Jernej Ule,Fernando Pastor,Lorea Blazquez,Puri FortesPubMed 35664701
- Disease markers 2022:92238832022Elevated Serum IGFBP-2 and CTGF Levels Are Associated with Disease Activity in Patients with Dermatomyositis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMin Yang,Yuehong Chen,Geng Yin,Sang Lin,Huan Liu,Yupeng Huang,Yunru Tian,Yueyuan Zhou,Fengming Luo,Qibing XiePubMed 35356065
- Clinical and translational radiation oncology 33:120-1272022The value of plasma hypoxia markers for predicting imaging-based hypoxia in patients with head-and-neck cancers undergoing definitive chemoradiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlexander Rühle,Anca-L Grosu,Nicole Wiedenmann,Juri Ruf,Birgit Bieber,Raluca Stoian,Andreas R Thomsen,Eleni Gkika,Peter Vaupel,Dimos Baltas,Wolfgang A Weber,Michael Mix,Nils H NicolayPubMed 35243023
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 93.75 - 6000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 64 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis.
Alternative names
CTGF, HCS24, IGFBP8, CCN2, CCN family member 2, Cellular communication network factor 2, Connective tissue growth factor, Hypertrophic chondrocyte-specific protein 24, Insulin-like growth factor-binding protein 8, IBP-8, IGF-binding protein 8, IGFBP-8
What's included?
Recommended products
Human CTGF ELISA kit is a single-wash 90-min Simplestep used to quantify Human CTGF with a sensitivity of 64 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 93.75 - 6000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 64 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 5.8 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 7.4 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 99 | Range 97 - 101 % |
Sample type EDTA Plasma | Average % = 121 | Range 117 - 124 % |
Sample type Cell culture media | Average % = 110 | Range 108 - 111 % |
Sample type Heparin Plasma | Average % = 129 | Range 127 - 130 % |
Sample type Citrate plasma | Average % = 100 | Range 94 - 105 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human CTGF ELISA kit ab261851 is a rapid single-wash 90-min ELISA kit to measure Human CTGF in cell culture media, serum and plasma. This SimpleStep ELISA® sensitivity is 64 pg/mL.
How the assay works
Human CTGF SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
This kit is designed for the quantification of human CTGF. The standard protein in this kit is mature full length human CTGF. Native signal was detected in serum, plasma (citrate), plasma (EDTA), and plasma (heparin)sample types. Spiked protein experiments were used to validate cell culture supernatant sample types. Saliva, urine, milk, CSF, cell extract, and tissue extract samples have not been tested with this kit.
Human CTGF ELISA kit ab261851 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm
How other researchers are using Human CTGF ELISA kit ab261851
Human CTGF ELISA kit ab261851 has been used to study connective tissue growth factor as a biomarker for hypertension (1) and the role of TGF-β1 in vascular calcification (2).
References:
(1)Xiaofei Li et al. 2023, PMID: 36843115,(2)Chih-Kang Chiang et al. 2023, PMID: 36787443
Save your precious samples
Our 384-well Human CTGF ELISA kit only requires a maxmum of 12.5µL
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
- Human CTGF Antibody Pair - BSA and Azide free Human CTGF Antibody Pair - BSA and Azide free ab270348
- Anti-CTGF antibody [EPR20728-240] - BSA and Azide free (Capture) Anti-CTGF antibody [EPR20728-240] - BSA and Azide free (Capture) ab269379
- Anti-CTGF antibody [EPR20728-214] - BSA and Azide free (Detector) Anti-CTGF antibody [EPR20728-214] - BSA and Azide free (Detector) ab259562
Related and recommended products
Human CTGF ELISA kit ab261851 is commonly used to study tissue repair and inflammation.
Other ELISA kits to measure fibrosis and extracellular matrix regulation include:
- Human Pro-Collagen I alpha 1 ELISA Kit Human Pro-Collagen I alpha 1 ELISA Kit ab210966
- Human IL-6 ELISA Kit Human IL-6 ELISA Kit ab178013
- Human Fibronectin ELISA kit Human Fibronectin ELISA Kit ab219046
- Human MMP1 ELISA kit Human MMP1 ELISA Kit ab215083
- Human Elastin ELISA kit Human Elastin ELISA Kit ab239433
Human connective tissue growth factor (CTGF), also known as CCN2, and Insulin-like growth factor-binding protein 8, is a matricellular protein encoded by the gene CTGF. Along with CYR61 (CCN1) and NOV (CCN3), CTGF belongs to the CCN family of extracellular matrix-associated heparin binding proteins. The CCN proteins contain IGFBP, Von Willebrand type C repeats, and thrombospodin type 1 repeat domains. CTGF has multiple functions including promotion proliferation and differentiation of chondrocytes; mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells, and enhances fibroblast growth factor-induced DNA synthesis.
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5 product images
Sandwich ELISA - Human CTGF ELISA kit (ab261851)
Serum from eight individual healthy human gender donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CTGF concentration was determined to be 18.8 ng/mL with a range of 11.32 – 25.5 ng/mL.
Sandwich ELISA - Human CTGF ELISA kit (ab261851)
Example of human CTGF standard curve in Sample Diluent 25BP.
The CTGF standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human CTGF ELISA kit (ab261851)
Interpolated concentrations of native CTGF in human serum and plasma samples.
The concentrations of CTGF were measured in duplicates, interpolated from the CTGF standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 10%, plasma (citrate) 5%, plasma (heparin) 5% and plasma (EDTA)10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CTGF concentration was determined to be 13,683 pg/mL in serum, 10,625 pg/mL in plasma (citrate) and 9,538 pg/mL in plasma (heparin) 5% and 10,607 pg/mL plasma (EDTA)10%.
Sandwich ELISA - Human CTGF ELISA kit (ab261851)
Example of human CTGF standard curve in Sample Diluent 25BP in 96-well vs. 384-well plate.
Sandwich ELISA - Human CTGF ELISA kit (ab261851)
Example of human CTGF standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
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