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AB325620

Human CTGF ELISA Kit, Chemiluminescent

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Human CTGF ELISA Kit, Chemiluminescent is a 55 min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human CTGF with higher sensitivity and broader measuring range.

- Single 30 min incubation and single -wash step for results in less than an hour
- Chemiluminescent detection allows a broad dynamic range : 21.95 - 16000 pg/ml with a sensitivity of 6.56 pg/mL
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Patent pending

View Alternative Names

CTGF, HCS24, IGFBP8, CCN2, CCN family member 2, Cellular communication network factor 2, Connective tissue growth factor, Hypertrophic chondrocyte-specific protein 24, Insulin-like growth factor-binding protein 8, IBP-8, IGF-binding protein 8, IGFBP-8

5 Images
Sandwich ELISA - Human CTGF ELISA Kit, Chemiluminescent (AB325620)
  • sELISA

Lab

Sandwich ELISA - Human CTGF ELISA Kit, Chemiluminescent (AB325620)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human CTGF ELISA Kit, Chemiluminescent (AB325620)
  • sELISA

Lab

Sandwich ELISA - Human CTGF ELISA Kit, Chemiluminescent (AB325620)

Standard curve comparison between the Chemiluminescent Human CTGF SimpleStep ELISA and colorimetric Human CTGF SimpleStep ELISA (ab261851) in Sample Diluent 25BP. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human CTGF ELISA Kit, Chemiluminescent (AB325620)
  • sELISA

Lab

Sandwich ELISA - Human CTGF ELISA Kit, Chemiluminescent (AB325620)

Dynamic range comparison between the Chemiluminescent Human CTGF SimpleStep ELISA, colorimetric Human CTGF SimpleStep ELISA (ab261851), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Sandwich ELISA - Human CTGF ELISA Kit, Chemiluminescent (AB325620)
  • sELISA

Lab

Sandwich ELISA - Human CTGF ELISA Kit, Chemiluminescent (AB325620)

Interpolated concentration of native CTGF was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 10%, plasma (citrate) 5%, plasma (heparin) 5%, and plasma (EDTA) 10%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent 25BP.

Sandwich ELISA - Human CTGF ELISA Kit, Chemiluminescent (AB325620)
  • sELISA

Lab

Sandwich ELISA - Human CTGF ELISA Kit, Chemiluminescent (AB325620)

Serum of eight individual healthy human donors was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean CTGF concentration was determined to be 18.8 ng/mL with a range of 11.32 – 25.5 ng/mL.

Key facts

Detection method

Luminescent

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture media

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 6.56 pg/mL

Range

21.95 - 16000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

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Product details

Human CTGF ELISA Kit, Chemiluminescent (ab325620) is a rapid single-wash sandwich 55 min ELISA to measure Human CTGF in Serum, Citrate Plasma, EDTA Plasma, Heparin Plasma, Cell Culture Media with a sensitivity of 6.56 pg/mL.

How the assay works

Human CTGF ELISA Kit, Chemiluminescent (ab325620) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colormetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human CTGF (ab325620) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-CTGF antibody [EPR20728-240] - BSA and Azide free (Capture) ab269379
- Anti-CTGF antibody [EPR20728-214] - BSA and Azide free (Detector) ab259562
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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 5.8" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 7.4" } ]
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Recovery

[ { "sample": "Serum", "range": "97 - 101 %", "average": "= 99" }, { "sample": "Citrate plasma", "range": "94 - 105 %", "average": "= 100" }, { "sample": "EDTA Plasma", "range": "117 - 124 %", "average": "= 121" }, { "sample": "Heparin Plasma", "range": "127 - 130 %", "average": "= 129" }, { "sample": "Cell culture media", "range": "108 - 111 %", "average": "= 110" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Is involved in the stimulation of osteoblast differentiation and has a critical role in osteogenesis (PubMed : 39414788). Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis.
See full target information CCN2
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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