AB274399

Human CTHRC1 ELISA Kit

Recombinant
SimpleStep
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(1 Publication)

Human CTHRC1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CTHRC1 with a sensitivity of 27.3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

UNQ762/PRO1550, CTHRC1, Collagen triple helix repeat-containing protein 1

5 Images

ELISA - Human CTHRC1 ELISA Kit (AB274399)

ELISA

Supplier Data

ELISA - Human CTHRC1 ELISA Kit (AB274399)

sELISA

Lab

Sandwich ELISA - Human CTHRC1 ELISA Kit (AB274399)

Interpolated concentration of native CTHRC1 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 50% serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human CTHRC1 ELISA Kit (AB274399)

Interpolated concentrations of native CTHRC1 in human serum, plasma and cell culture supernatant samples.

The concentrations of CTHRC1 were measured in duplicates, interpolated from the CTHRC1 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, and SK-MEL28 cell culture supernatant 4%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CTHRC1 concentration was determined to be 9,486 pg/mL in serum, 10,463 pg/mL in plasma (citrate) and 140,962 pg/mL in SK-MEL28 cell culture supernatant.

sELISA

Lab

Sandwich ELISA - Human CTHRC1 ELISA Kit (AB274399)

Example of human CTHRC1 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human CTHRC1 ELISA Kit (AB274399)

Example of human CTHRC1 standard curve in Sample Diluent NS.

The CTHRC1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 27.3 pg/mL

Range

93.75 - 6000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human CTHRC1 ELISA Kit ab274399 is a rapid single-wash 90-min Sandwich ELISA to measure Human CTHRC1 in cell culture supernatant, citrate plasma, serum. This SimpleStep sensitivity is 27.3 pg/mL.

How the assay works

Human CTHRC1 SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human CTHRC1 ELISA Kit ab274399 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Save your precious samples

Our 384-well Human CTHRC1 ELISA Kit only requires a maximum of 12.5µL sample

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-CTHRC1 antibody [EPR22851-144] - BSA and Azide free (Capture) ab274447
- Anti-CTHRC1 antibody [EPR22851-215] - BSA and Azide free (Detector) ab274447

Collagen triple helix repeat containing protein 1 (CTHRC1) is a 30 kDa secreted glycoprotein encoded by the CTHRC1 gene. CTHRC1 is known to regulate extracellular collagen deposition and partake in vascular remodeling of arterial injury as a function of cellular response through the TGF-? and Wnt pathways. Moreover, mutation of the gene has been associated with carcinogenesis in a variety of cell and tissues, most notably esophageal adenocarcinoma and Barrett's esophagus. Furthermore, recent studies have demonstrated CTHRC1 as a marker of the severity of the disease progression in the dystrophic muscles.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.88" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.55" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "99 - 101 %", "average": "= 100" }, { "sample": "Serum", "range": "91 - 101 %", "average": "= 97" }, { "sample": "Citrate plasma", "range": "95 - 103 %", "average": "= 100" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The CTHRC1 protein also known as Collagen Triple Helix Repeat Containing 1 is a secreted protein with a mass of approximately 28 kDa. It plays a mechanical role in the extracellular matrix by modulating collagen fibrillogenesis and cross-linking. Increased expression of CTHRC1 protein often appears in areas undergoing active remodeling like those around wound healing sites fibrotic lesions and certain tumor tissues. The expression is not uniform across tissues and often corresponds to areas requiring structural reorganization or repair.

Biological function summary

CTHRC1 impacts cell migration and invasion. It associates with several extracellular matrix components influencing cellular response through cytoskeletal reorganization. There is no evidence for its participation as a part of a larger protein complex. The modulation of collagen interactions by CTHRC1 enables it to play a significant role in angiogenesis and tissue repair processes affecting endothelial and fibroblast cell functions.

Pathways

CTHRC1 participates in signaling pathways related to Transforming Growth Factor-beta (TGF-?) and Wnt. In the TGF-? pathway it impacts cellular responses to growth signals enhancing remodeling and repair mechanisms. Through the Wnt signaling pathway CTHRC1 interacts with Dishevelled proteins assisting in regulation and pathway activation related to cell proliferation and migration. These pathways highlight its involvement in important biological processes that influence tissue structure and cell communication.

CTHRC1 has relevance to both cancer progression and fibrotic diseases. Its overexpression is often noted in breast cancer where it drives tumor growth and metastasis interacting with other matrix components. Similarly in fibrosis CTHRC1 contributes to excessive collagen deposition and tissue stiffness. Proteins like TGF-? intersect with CTHRC1 functions further modulating disease pathway outcomes. Understanding CTHRC1's role in these disorders could inform therapeutic targets for modulating matrix dynamics.

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Product protocols

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Target data

May act as a negative regulator of collagen matrix deposition.

See full target information CTHRC1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Medicina (Kaunas, Lithuania) 59: PubMed37109608

2023

Elevated Levels of Plasma Collagen Triple Helix Repeat Containing 1 (CTHRC1) Are Strongly Associated with eGFR and Albuminuria in Chronic Kidney Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Yeldar Baiken,Zhannur Markhametova,Assem Ashimova,Ainur Zhulamanova,Assem Nogaibayeva,Larissa Kozina,Bakhyt Matkarimov,Bauyrzhan Aituov,Abduzhappar Gaipov,Askhat Myngbay

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human CTHRC1 ELISA Kit

ELISA - Human CTHRC1 ELISA Kit (AB274399)

Sandwich ELISA - Human CTHRC1 ELISA Kit (AB274399)

Sandwich ELISA - Human CTHRC1 ELISA Kit (AB274399)

Sandwich ELISA - Human CTHRC1 ELISA Kit (AB274399)

Sandwich ELISA - Human CTHRC1 ELISA Kit (AB274399)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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