Human CTHRC1 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human CTHRC1 in Citrate plasma, Cell culture supernatant, Serum samples.
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- Medicina (Kaunas, Lithuania) 59:2023Elevated Levels of Plasma Collagen Triple Helix Repeat Containing 1 (CTHRC1) Are Strongly Associated with eGFR and Albuminuria in Chronic Kidney Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYeldar Baiken,Zhannur Markhametova,Assem Ashimova,Ainur Zhulamanova,Assem Nogaibayeva,Larissa Kozina,Bakhyt Matkarimov,Bauyrzhan Aituov,Abduzhappar Gaipov,Askhat MyngbayPubMed 37109608
Key facts
- Detection method
- Colorimetric
- Sample types
- Citrate plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 93.75 - 6000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 27.3 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
May act as a negative regulator of collagen matrix deposition.
Alternative names
UNQ762/PRO1550, CTHRC1, Collagen triple helix repeat-containing protein 1
What's included?
Recommended products
Human CTHRC1 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human CTHRC1 in Citrate plasma, Cell culture supernatant, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Citrate plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 93.75 - 6000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 27.3 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Supernatant | n 8 | mean - | SD - | C.V. 2.55 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Supernatant | n 3 | mean - | SD - | C.V. 2.88 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 100 | Range 99 - 101 % |
Sample type Serum | Average % = 97 | Range 91 - 101 % |
Sample type Citrate plasma | Average % = 100 | Range 95 - 103 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human CTHRC1 ELISA kit (ab274399) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human CTHRC1 protein in serum, plasma and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CTHRC1 with 27.3 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Collagen triple helix repeat containing protein 1 (CTHRC1) is a 30 kDa secreted glycoprotein encoded by the CTHRC1 gene. CTHRC1 is known to regulate extracellular collagen deposition and partake in vascular remodeling of arterial injury as a function of cellular response through the TGF-β and Wnt pathways. Moreover, mutation of the gene has been associated with carcinogenesis in a variety of cell and tissues, most notably esophageal adenocarcinoma and Barrett's esophagus. Furthermore, recent studies have demonstrated CTHRC1 as a marker of the severity of the disease progression in the dystrophic muscles.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The CTHRC1 protein also known as Collagen Triple Helix Repeat Containing 1 is a secreted protein with a mass of approximately 28 kDa. It plays a mechanical role in the extracellular matrix by modulating collagen fibrillogenesis and cross-linking. Increased expression of CTHRC1 protein often appears in areas undergoing active remodeling like those around wound healing sites fibrotic lesions and certain tumor tissues. The expression is not uniform across tissues and often corresponds to areas requiring structural reorganization or repair.
- Biological function summary
CTHRC1 impacts cell migration and invasion. It associates with several extracellular matrix components influencing cellular response through cytoskeletal reorganization. There is no evidence for its participation as a part of a larger protein complex. The modulation of collagen interactions by CTHRC1 enables it to play a significant role in angiogenesis and tissue repair processes affecting endothelial and fibroblast cell functions.
- Pathways
CTHRC1 participates in signaling pathways related to Transforming Growth Factor-beta (TGF-β) and Wnt. In the TGF-β pathway it impacts cellular responses to growth signals enhancing remodeling and repair mechanisms. Through the Wnt signaling pathway CTHRC1 interacts with Dishevelled proteins assisting in regulation and pathway activation related to cell proliferation and migration. These pathways highlight its involvement in important biological processes that influence tissue structure and cell communication.
- Associated diseases and disorders
CTHRC1 has relevance to both cancer progression and fibrotic diseases. Its overexpression is often noted in breast cancer where it drives tumor growth and metastasis interacting with other matrix components. Similarly in fibrosis CTHRC1 contributes to excessive collagen deposition and tissue stiffness. Proteins like TGF-β intersect with CTHRC1 functions further modulating disease pathway outcomes. Understanding CTHRC1's role in these disorders could inform therapeutic targets for modulating matrix dynamics.
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4 product images
Sandwich ELISA - Human CTHRC1 ELISA Kit (ab274399)
Example of human CTHRC1 standard curve in Sample Diluent NS.
The CTHRC1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human CTHRC1 ELISA Kit (ab274399)
Interpolated concentrations of native CTHRC1 in human serum, plasma and cell culture supernatant samples.
The concentrations of CTHRC1 were measured in duplicates, interpolated from the CTHRC1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, and SK-MEL28 cell culture supernatant 4%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CTHRC1 concentration was determined to be 9,486 pg/mL in serum, 10,463 pg/mL in plasma (citrate) and 140,962 pg/mL in SK-MEL28 cell culture supernatant.
Sandwich ELISA - Human CTHRC1 ELISA Kit (ab274399)
Example of human CTHRC1 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human CTHRC1 ELISA Kit (ab274399)
Interpolated concentration of native CTHRC1 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 50% serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
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