AB264616

Human CTLA-4 ELISA Kit

Recombinant
SimpleStep
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(1 Publication)

Human CTLA-4 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CTLA-4 with a sensitivity of 13.2 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD152, CTLA4, Cytotoxic T-lymphocyte protein 4, Cytotoxic T-lymphocyte-associated antigen 4, CTLA-4

3 Images

Sandwich ELISA - Human CTLA-4 ELISA Kit (AB264616)

sELISA

Supplier Data

Sandwich ELISA - Human CTLA-4 ELISA Kit (AB264616)

Interpolated concentration of native CTLA-4 in human thymus tissue extract based on a 250 μg/mL extract load.

The concentration of CTLA-4 were measured in duplicate and interpolated from the CTLA-4 standard curve. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CTLA-4 concentration was determined to be 66.2 pg/mL in thymus sample

sELISA

Supplier Data

Sandwich ELISA - Human CTLA-4 ELISA Kit (AB264616)

Representative image of Human CTLA-4 ELISA Kit (ab264616)

Components shown from left to right :

- Sample diluent NS

- 10X Wash Buffer PT (ab206977)

- 10X Human CTLA-4 Detector Antibody

- Human CTLA-4 Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Human CTLA-4 Capture Antibody (Lyophilised) (2 vials)

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody Diluent 4BI

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

sELISA

Supplier Data

Sandwich ELISA - Human CTLA-4 ELISA Kit (AB264616)

Example of human CTLA-4 standard curve in Sample Diluent NS.

The CTLA-4 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, Cell Lysate, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 13.2 pg/mL

Range

46.88 - 3000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human CTLA-4 ELISA Kit (ab264616) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CTLA-4 protein in cell culture supernatant, cell lysate, cit plasma, edta plasma, hep plasma, serum, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CTLA-4 with 13.2 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Cytotoxic T lymphocyte antigen-4 (CTLA-4) is a transmembrane protein expressed on T regulator cells. CTLA-4 is a negative regulator of T cell activation. CTLA-4 binds markers on antigen presenting cells (APC) including B7, which functions as an immune check point to down regulate the immune response. Blocking CTLA-4 allows for activation and proliferation of more T cells which reduces the mediated immunosuppression. CTLA-4 soluble extracellular domain in serum and plasma is an indication of autoimmune diseases.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.4" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.9" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "95 - 98 %", "average": "= 97" }, { "sample": "Serum", "range": "93 - 98 %", "average": "= 95" }, { "sample": "EDTA Plasma", "range": "87 - 92 %", "average": "= 89" }, { "sample": "Heparin Plasma", "range": "91 - 95 %", "average": "= 93" }, { "sample": "Citrate plasma", "range": "88 - 96 %", "average": "= 91" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab264616-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB264616-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB264616-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB264616-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB264616-CMP08", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB264616-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB264616-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB264616-CMP11", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB264616-CMP10", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Lyophilized Human CTLA-4 Capture Antibody", "number":"AB264616-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human CTLA-4 Lyophilized Recombinant Protein", "number":"AB264616-CMP07", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB264616-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CTLA-4 Detector Antibody", "number":"AB264616-CMP01", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CTLA-4 also known as CD152 is an immune checkpoint receptor with a molecular weight of approximately 34 kDa. This protein is expressed on the surface of T-cells especially after activation and sometimes on regulatory T-cells (Tregs). CTLA-4 competes with CD28 for binding to the same ligands CD80 and CD86 on antigen-presenting cells. Unlike CD28 which stimulates T-cell activation CTLA-4 sends inhibitory signals to downregulate immune responses. By doing this CTLA-4 helps maintain immune system homeostasis and prevents autoimmune reactions.

Biological function summary

The CTLA-4 protein functions as a critical regulator of the immune system. It interacts with CD80/CD86 in a complex that effectively transmits inhibitory signals to T-cells. CTLA-4 controls the amplitude of the initial activation of T-cells ensuring that the body's immune responses are kept under control. In regulatory T-cells CTLA-4 engagement contributes to their immunosuppressive functions through which they maintain tolerance to self-antigens and prevent overactive immune reactions.

Pathways

CTLA-4 involves itself in the adaptive immune response pathways specifically the regulation of T-cell activity. CTLA-4's interaction with CD80/CD86 modulates pathways associated with TCR (T-cell receptor) signaling. When CTLA-4 binds its ligands it recruits phosphatases such as SHP-2 that dephosphorylate signaling proteins leading to the downregulation of T-cell interaction. This pathway interaction exemplifies how CTLA-4 acts in concert with proteins like CD28 to finely tune immune responses.

CTLA-4 plays a role in autoimmune diseases and cancer. The dysregulation of CTLA-4 function can lead to autoimmune disorders where the immune system attacks the body's own tissue. In cancer tumor cells may manipulate CTLA-4 pathways to evade immune surveillance. Targeted therapies like anti-CTLA-4 antibodies such as ipilimumab have been developed to block CTLA-4 aiming to enhance the immune system’s ability to attack cancer cells. These treatments highlight the role of CTLA-4 in balancing immune activity and the potential to alter this balance for therapeutic benefit.

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Product protocols

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Target data

Inhibitory receptor acting as a major negative regulator of T-cell responses (PubMed : 11279501, PubMed : 11279502, PubMed : 16551244, PubMed : 1714933, PubMed : 18641304, PubMed : 28484017). Acts as a decoy receptor : the affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28 (PubMed : 11279501, PubMed : 11279502, PubMed : 16551244, PubMed : 1714933, PubMed : 28484017).

See full target information CTLA4

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer medicine 12:17648-17659 PubMed37501393

2023

Serum soluble immune checkpoint levels predict cervical lymph node metastasis of differentiated thyroid carcinoma patients.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Shao,Xinru Gui,Yuxin Wang,Lei Sheng,Dong Sun,Qingdong Zeng,Huayang Wang

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human CTLA-4 ELISA Kit

Sandwich ELISA - Human CTLA-4 ELISA Kit (AB264616)

Sandwich ELISA - Human CTLA-4 ELISA Kit (AB264616)

Sandwich ELISA - Human CTLA-4 ELISA Kit (AB264616)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Assay Kits

Assay Kits

Assay Kits

Assay Kits

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise