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AB309454

Human CTRP4 ELISA Kit

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Human CTRP4 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human CTRP4 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

CTRP4, C1QTNF4, Complement C1q tumor necrosis factor-related protein 4, C1q/TNF-related protein 4

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Sandwich ELISA - Human CTRP4 ELISA Kit (AB309454)
  • sELISA

Supplier Data

Sandwich ELISA - Human CTRP4 ELISA Kit (AB309454)

Example of standard curve using ab309454. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.14 ng/mL

Range

0.14 - 40 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human CTRP4 ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CTRP4 in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for Human CTRP4 coated on a 96-well plate.
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Recovery

[ { "sample": "Serum", "range": "70 - 131 %", "average": "= 97.55" }, { "sample": "Plasma", "range": "70 - 124 %", "average": "= 96.45" }, { "sample": "Cell culture supernatant", "range": "71 - 118 %", "average": "= 92.62" }, { "sample": "Cell culture media", "range": "71 - 118 %", "average": "= 92.62" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab309454-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent D (Item K)", "number":"AB309454-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human CTRP4 Antibody-coated ELISA Plate", "number":"AB309454-CMP15", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human CTRP4 Protein Standard", "number":"AB309454-CMP16", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB309454-CMP08", "productcode":"" }, { "size":"1 x 200 µL", "name":"100X HRP-Streptavidin", "number":"AB309454-CMP13", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB309454-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human CTRP4 Detection Antibody", "number":"AB309454-CMP14", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB309454-CMP02", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B (Item E)", "number":"AB309454-CMP10", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CTRP4 also known as C1q and tumor necrosis factor-related protein 4 is a member of the C1q/TNF-related protein family. It has a molecular weight of approximately 31 kDa. The protein is expressed in various tissues including adipose tissue and the liver where it performs regulatory functions by interacting with other cellular components. Its presence in these tissues suggests roles in metabolism and inflammation.
Biological function summary

CTRP4 functions as a modulator of immune responses and energy balance. It is involved in forming complexes that can influence inflammatory pathways playing a part in controlling cytokine production. As a member of the CTRP family CTRP4 shares structural homology that allows it to interact with receptors implicated in metabolic and immune processes. This connection points to its role in maintaining homeostasis.

Pathways

CTRP4 participates in metabolic and inflammatory signaling networks. It engages in the AMPK signaling pathway which is critical for energy regulation. Within this context CTRP4 acts together with proteins like adiponectin to modulate glucose uptake and lipid metabolism. In the inflammatory landscape CTRP4 influences the MAPK signaling pathway affecting cellular responses to various stressors and regulatory signals.

CTRP4 has associations with obesity and type 2 diabetes. Its role in these conditions links to its function in metabolic regulation and inflammation control. For instance CTRP4 modulates inflammatory profiles connecting it with proteins such as TNF-alpha in the context of metabolic syndrome. Research continues to explore its potential as a therapeutic target given its involvement in these significant health challenges.
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Product protocols

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Target data

May be involved in the regulation of the inflammatory network. Its role as pro- or anti-inflammatory seems to be context dependent (PubMed : 21658842, PubMed : 27086950). Seems to have some role in regulating food intake and energy balance when administered in the brain. This effect is sustained over a two-day period, and it is accompanied by decreased expression of orexigenic neuropeptides in the hypothalamus 3 hours post-injection (By similarity).
See full target information C1QTNF4
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websiteProtocolBooklet
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Complementary Products

Primary Antibodies

AB36871

Anti-CTRP4 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTRP4 antibody (AB36871)

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