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AB313517

Human CTRP5 ELISA Kit

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Human CTRP5 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human CTRP5 in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

CTRP5, UNQ303/PRO344, C1QTNF5, Complement C1q tumor necrosis factor-related protein 5

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Sandwich ELISA - Human CTRP5 ELISA Kit (AB313517)
  • sELISA

Supplier Data

Sandwich ELISA - Human CTRP5 ELISA Kit (AB313517)

Example of standard curve using ab313517. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.82 ng/mL

Range

0.82 - 200 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human CTRP5 ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CTRP5 in serum (human CTRP5 concentration is low in normal serum/plasma, and may not be detectable in this assay concentration), plasma (collect plasma using heparin or citrate as an anticoagulant. EDTA is not recommended as anticoagulant for use in this assay), and cell culture supernatants. This assay employs an antibody specific for human CTRP5 coated on a 96-well plate.
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Recovery

[ { "sample": "Cell culture media", "range": "69 - 143 %", "average": "= 104.1" }, { "sample": "Plasma", "range": "70 - 89 %", "average": "= 76.5" }, { "sample": "Serum", "range": "68 - 88 %", "average": "= 75.86" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab313517-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent B (Item E)", "number":"AB313517-CMP06", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB313517-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human CTRP5 Detection Antibody", "number":"AB313517-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human CTRP5 Protein Standard", "number":"AB313517-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human CTRP5 Antibody-coated ELISA Plate", "number":"AB313517-CMP09", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB313517-CMP07", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A (Item D)", "number":"AB313517-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB313517-CMP08", "productcode":"" }, { "size":"1 x 200 µL", "name":"300X HRP-Streptavidin", "number":"AB313517-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C1q/tumor necrosis factor-related protein 5 (CTRP5) also known as cysteine-rich with epidermal growth factor-like domains 1 (C1qLF) or hCTRP5 is a secreted protein with a molecular mass of approximately 25 kDa. It belongs to the CTRP family and exhibits a similar domain architecture to adiponectin. CTRP5 is expressed mainly in ocular tissues such as the retina and choroid as well as in adipose tissue. Its secreted nature suggests an involvement in intercellular communication.
Biological function summary

The CTRP5 protein regulates metabolic processes and immune responses. It functions as part of a homotrimeric complex interacting with other proteins to modulate tissue homeostasis. CTRP5 plays a role in lipid metabolism contributing to cholesterol and fatty acid handling. In immune response modulation it influences inflammation acting as a signal modulator in macrophages and other immune cells. Its presence in eye tissue suggests it has a specific role in maintaining ocular health.

Pathways

The CTRP5 protein integrates into the complement and inflammation pathways. It participates in the complement cascade influencing immune responses. CTRP5 interacts with proteins such as C1q and complement receptors indicating its involvement in regulating inflammation through the classical pathway. It also contributes to metabolic pathways related to adiponectin signaling highlighting its connection to lipid regulation and energy balance in tissues.

The CTRP5 protein has connections to age-related macular degeneration (AMD) and late-onset retinal degeneration (L-ORMD). Mutations in CTRP5 are associated with these ocular conditions affecting visual function. In AMD CTRP5 interactions with C1q and other complement components indicate a role in aberrant immune activation within the retina. Understanding CTRP5 in these contexts could provide insights into potential therapeutic strategies for these vision-threatening disorders.
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Product protocols

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Target data

See full target information C1QTNF5
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websiteProtocolBooklet
en
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Complementary Products

Primary Antibodies

AB36893

Anti-CTRP5 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTRP5 antibody (AB36893)

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Proteins & Peptides

AB109041

Recombinant human CTRP5 protein

Bioactive

Functional Studies - Recombinant human CTRP5 protein (AB109041)

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