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AB100530

Human CXCL1 ELISA Kit (GRO alpha)

5

(1 Review)

|

(2 Publications)

Human CXCL1 ELISA Kit (GRO alpha) is a Sandwich (quantitative) ELISA for the measurement of Human CXCL1 (GRO alpha) in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

GRO, GRO1, GROA, MGSA, SCYB1, CXCL1, Growth-regulated alpha protein, C-X-C motif chemokine 1, GRO-alpha(1-73), Melanoma growth stimulatory activity, Neutrophil-activating protein 3, NAP-3

1 Images
Sandwich ELISA - Human CXCL1 ELISA Kit (GRO alpha) (AB100530)
  • sELISA

Unknown

Sandwich ELISA - Human CXCL1 ELISA Kit (GRO alpha) (AB100530)

Representative standard curve using ab100530

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 2 pg/mL

Range

2 - 1000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's CXCL1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit (GRO alpha) is an in vitro enzyme designed for the quantitative measurement of Human GRO alpha in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Human CXCL1 (GRO alpha) coated on a 96-well plate. Standards and samples are pipetted into the wells and CXCL1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CXCL1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CXCL1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Get higher sensitivity in only 90 minutes with Human CXCL1 ELISA Kit (GRO alpha) (ab190805) from our SimpleStep ELISA® range.

Optimization may be required with urine samples

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Recovery

[ { "sample": "Cell culture supernatant", "range": "101 - 122 %", "average": "= 112.2" }, { "sample": "Serum", "range": "119 - 145 %", "average": "= 130.4" }, { "sample": "Plasma", "range": "85 - 140 %", "average": "= 113.5" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100530-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"GRO-alpha Microplate (12 x 8 wells)", "number":"AB100530-CMP06", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB100530-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100530-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human GRO-alpha", "number":"AB100530-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human GRO-alpha Standard (lyophilized)", "number":"AB100530-CMP07", "productcode":"" }, { "size":"1 x 200 µL", "name":"400X HRP-Streptavidin Concentrate", "number":"AB100530-CMP02", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100530-CMP04", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100530-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CXCL1 also known as GRO alpha or KC in certain species is a chemokine with a molecular mass of around 7.8 kDa. This protein is primarily secreted by macrophages neutrophils and endothelial cells. It plays a critical role in the recruitment of neutrophils during inflammation and acts by binding to the CXCR2 receptor. CXCL1 is expressed in many tissues including lungs liver and skin where it regulates the immune response and facilitates tissue repair.
Biological function summary

CXCL1 functions as a significant mediator in inflammatory processes. It does not form a complex but acts independently to exert its effects. By attracting neutrophils to sites of injury or infection CXCL1 supports the body's defense mechanisms. This chemokine also influences angiogenesis contributing to the formation of new blood vessels which is essential in wound healing and tissue regeneration.

Pathways

CXCL1 plays a role in both the NF-kB signaling and MAPK signaling pathways. These pathways are pivotal for the regulation of immune and inflammatory responses. CXCL1 acts alongside proteins such as IL-8 another chemokine that also binds to the CXCR2 receptor. Through these pathways CXCL1 influences the activation and migration of immune cells facilitating a rapid response to inflammatory stimuli.

CXCL1 has a significant association with conditions such as chronic obstructive pulmonary disease (COPD) and rheumatoid arthritis. In COPD elevated levels of CXCL1 contribute to persistent inflammation and neutrophil infiltration. In rheumatoid arthritis CXCL1 participates in joint inflammation and damage interacting with proteins like TNF-alpha which amplifies inflammatory responses. Understanding the role of CXCL1 in these conditions could help guide the development of targeted therapies to mitigate inflammation and tissue damage.
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Product protocols

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Target data

Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.
See full target information CXCL1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Basic research in cardiology 115:67 PubMed33185739

2020

Functional investigation of the coronary artery disease gene SVEP1.

Applications

Unspecified application

Species

Unspecified reactive species

Michael J Winkler,Philipp Müller,Amin M Sharifi,Jana Wobst,Hanna Winter,Michal Mokry,Lijiang Ma,Sander W van der Laan,Shichao Pang,Benedikt Miritsch,Julia Hinterdobler,Julia Werner,Barbara Stiller,Ulrich Güldener,Tom R Webb,Folkert W Asselbergs,Johan L M Björkegren,Lars Maegdefessel,Heribert Schunkert,Hendrik B Sager,Thorsten Kessler

Journal of cell science 130:190-202 PubMed27656113

2016

Increased ROS production in non-polarized mammary epithelial cells induces monocyte infiltration in 3D culture.

Applications

Unspecified application

Species

Unspecified reactive species

Linzhang Li,Jie Chen,Gaofeng Xiong,Daret K St Clair,Wei Xu,Ren Xu
View all publications
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websiteProtocolBooklet
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