Human CXCL1 ELISA Kit (GRO alpha)

Human CXCL1 ELISA Kit (ab190805) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CXCL1 protein in cell culture supernatant, cit plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CXCL1 with 0.064 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

CXCL1 (GRO alpha) is a member of the CXC family of chemokines. CXCL1 contains the ELR motif and preferentially chemoattracts neutrophils. Human CXCL1 undergoes post-translational modification into three isoforms called CXCL1(4-73), CXCL1(5-73) and CXCL1(6-73). These isoforms are produced by proteolytic cleavage after secretion from peripheral blood monocytes, and all show higher activity in-vitro than the full-length protein. Two additional CXC proteins, CXCL2 (or GRO beta) and CXCL3 (or GRO gamma), share 90% and 86% amino acid sequence homology, respectively, with CXCL1. Human CXCL1 mRNA is expressed in foreskin fibroblasts, synovial fibroblasts, chondrocytes and osteocytes. Additionally, CXCL1 mRNA has been detected in mammary fibroblasts and epithelial cells, endothelial cells, activated monocytes, macrophages, and neutrophils. CXCL1 is a growth-regulated gene and is overexpressed constitutively in tumorigenic cells. As a result, elevated levels of CXCL1 can be seen in several types of tumors and cancers, including lung cancer and melanoma.