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AB190805

Human CXCL1 ELISA Kit (GRO alpha)

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(12 Publications)

Human CXCL1 ELISA Kit (GRO alpha) is a single-wash 90-min Simplestep used to quantify Human CXCL1 (GRO alpha) with a sensitivity of 0.064 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

GRO, GRO1, GROA, MGSA, SCYB1, CXCL1, Growth-regulated alpha protein, C-X-C motif chemokine 1, GRO-alpha(1-73), Melanoma growth stimulatory activity, Neutrophil-activating protein 3, NAP-3

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ELISA - Human CXCL1 ELISA Kit (GRO alpha) (AB190805)
  • ELISA

Supplier Data

ELISA - Human CXCL1 ELISA Kit (GRO alpha) (AB190805)
Sandwich ELISA - Human CXCL1 ELISA Kit (GRO alpha) (AB190805)
  • sELISA

Supplier Data

Sandwich ELISA - Human CXCL1 ELISA Kit (GRO alpha) (AB190805)

Specificity of CXCL1 (GRO alpha) signal on unstimulated and stimulated media supernatants.

Human PBMCs were cultured in RPMI supplemented with 10% fetal calf serum, 2 mM L-glutamine. Cells were cultured for 2 days at 37˚C in the presence or absence of 1.5% PHA-M. The concentrations of CXCL1 (GRO alpha) were interpolated from the standard curve in Sample Diluent NS and corrected for sample dilution. The mean CXCL1 concentration was determined to be 5.11 pg/mL in unstimulated PBMC supernatants, and 17533.21 pg/mL in stimulated PBMC supernatants.

Sandwich ELISA - Human CXCL1 ELISA Kit (GRO alpha) (AB190805)
  • sELISA

Supplier Data

Sandwich ELISA - Human CXCL1 ELISA Kit (GRO alpha) (AB190805)

Example of human CXCL1 (GRO alpha) standard curve in Sample Diluent NS.

The CXCL1 (GRO alpha) standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human CXCL1 ELISA Kit (GRO alpha) (AB190805)
  • sELISA

Supplier Data

Sandwich ELISA - Human CXCL1 ELISA Kit (GRO alpha) (AB190805)

Interpolated concentrations of human CXCL1 in serum, platelet poor plasma (citrate), platelet poor plasma (heparin), and CSF.

Interpolated concentration of native CXCL1 was measured in duplicate at different sample concentrations. Undiluted samples are 100% serum, 100% platelet poor plasma (citrate), 100% platelet poor plasma (heparin), and 50% CSF. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human CXCL1 ELISA Kit (GRO alpha) (AB190805)
  • sELISA

Supplier Data

Sandwich ELISA - Human CXCL1 ELISA Kit (GRO alpha) (AB190805)

Interpolated concentrations of native CXCL1 (GRO alpha) in human PBMC supernatant from cells treated with 1.5% PHA-M for 46 hours.

The concentrations of CXCL1 (GRO alpha) were measured in duplicate and interpolated from the CXCL1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL1 concentration was determined to be 17.53 ng/mL in treated PBMC supernatant.

Sandwich ELISA - Human CXCL1 ELISA Kit (GRO alpha) (AB190805)
  • sELISA

Supplier Data

Sandwich ELISA - Human CXCL1 ELISA Kit (GRO alpha) (AB190805)

Human CXCL1 (GRO alpha) Standard Curve Comparison between new and original ELISA kit.

Standard Curve comparison between new human CXCL1 SimpleStep ELISA kit (GRO alpha) and original ELISA kit. The current SimpleStep ELISA kit shows increased sensitivity.

Sandwich ELISA - Human CXCL1 ELISA Kit (GRO alpha) (AB190805)
  • sELISA

Supplier Data

Sandwich ELISA - Human CXCL1 ELISA Kit (GRO alpha) (AB190805)

Human CXCL1 (GRO alpha) Standard Curve Comparison.

Standard Curve comparison between human CXCL1 SimpleStep ELISA kit (GRO alpha) and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.064 pg/mL

Range

2.34 - 150 pg/mL

Assay time

1h 30m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human CXCL1 ELISA Kit (GRO alpha) ab190805 is a rapid single-wash 90-min Sandwich ELISA to measure Human CXCL1 (GRO alpha)in cell culture supernatant, CSF, citrate plasma, heparin plasma, serum. This SimpleStep sensitivity is 0.064 pg/mL.

How the assay works

Human CXCL1 (GRO alpha)SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human CXCL1 ELISA Kit (GRO alpha) ab190805 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

CXCL1 (GRO alpha) is a member of the CXC family of chemokines. CXCL1 contains the ELR motif and preferentially chemoattracts neutrophils. Human CXCL1 undergoes post-translational modification into three isoforms called CXCL1(4-73), CXCL1(5-73) and CXCL1(6-73). These isoforms are produced by proteolytic cleavage after secretion from peripheral blood monocytes, and all show higher activity in-vitro than the full-length protein. Two additional CXC proteins, CXCL2 (or GRO beta) and CXCL3 (or GRO gamma), share 90% and 86% amino acid sequence homology, respectively, with CXCL1. Human CXCL1 mRNA is expressed in foreskin fibroblasts, synovial fibroblasts, chondrocytes and osteocytes. Additionally, CXCL1 mRNA has been detected in mammary fibroblasts and epithelial cells, endothelial cells, activated monocytes, macrophages, and neutrophils. CXCL1 is a growth-regulated gene and is overexpressed constitutively in tumorigenic cells. As a result, elevated levels of CXCL1 can be seen in several types of tumors and cancers, including lung cancer and melanoma.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.7" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.9" } ]
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Recovery

[ { "sample": "Cerebral Spinal Fluid", "range": "93 - 101 %", "average": "= 96" }, { "sample": "Cell culture supernatant", "range": "92 - 97 %", "average": "= 95" }, { "sample": "Serum", "range": "82 - 85 %", "average": "= 83" }, { "sample": "Heparin Plasma", "range": "85 - 87 %", "average": "= 86" }, { "sample": "Citrate plasma", "range": "80 - 82 %", "average": "= 81" } ]
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What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab190805-10x96Tests", "assetComponentDetails": [ { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB190805-CMP04" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB190805-CMP10" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB190805-CMP05" }, { "size":"2 x 10 Vial", "name":"Human CXCL1 Lyophilized Recombinant Protein (ab9806)", "number":"AB190805-CMP08" }, { "size":"1 x 6000 µL", "name":"10X Human CXCL1 Capture Antibody", "number":"AB190805-CMP06" }, { "size":"10 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB190805-CMP01" }, { "size":"2 x 250 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB190805-CMP02" }, { "size":"10 x 1 Unit", "name":"Plate Seals", "number":"AB190805-CMP09" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB190805-CMP03" }, { "size":"1 x 6000 µL", "name":"10X Human CXCL1 Detector Antibody", "number":"AB190805-CMP07" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab190805-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB190805-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB190805-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB190805-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB190805-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB190805-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CXCL1 Capture Antibody", "number":"AB190805-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human CXCL1 Lyophilized Recombinant Protein (ab9806)", "number":"AB190805-CMP08", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB190805-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CXCL1 Detector Antibody", "number":"AB190805-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB190805-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CXCL1 also known as GRO alpha or KC in certain species is a chemokine with a molecular mass of around 7.8 kDa. This protein is primarily secreted by macrophages neutrophils and endothelial cells. It plays a critical role in the recruitment of neutrophils during inflammation and acts by binding to the CXCR2 receptor. CXCL1 is expressed in many tissues including lungs liver and skin where it regulates the immune response and facilitates tissue repair.
Biological function summary

CXCL1 functions as a significant mediator in inflammatory processes. It does not form a complex but acts independently to exert its effects. By attracting neutrophils to sites of injury or infection CXCL1 supports the body's defense mechanisms. This chemokine also influences angiogenesis contributing to the formation of new blood vessels which is essential in wound healing and tissue regeneration.

Pathways

CXCL1 plays a role in both the NF-kB signaling and MAPK signaling pathways. These pathways are pivotal for the regulation of immune and inflammatory responses. CXCL1 acts alongside proteins such as IL-8 another chemokine that also binds to the CXCR2 receptor. Through these pathways CXCL1 influences the activation and migration of immune cells facilitating a rapid response to inflammatory stimuli.

CXCL1 has a significant association with conditions such as chronic obstructive pulmonary disease (COPD) and rheumatoid arthritis. In COPD elevated levels of CXCL1 contribute to persistent inflammation and neutrophil infiltration. In rheumatoid arthritis CXCL1 participates in joint inflammation and damage interacting with proteins like TNF-alpha which amplifies inflammatory responses. Understanding the role of CXCL1 in these conditions could help guide the development of targeted therapies to mitigate inflammation and tissue damage.
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Product protocols

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Target data

Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.
See full target information CXCL1
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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

World journal of gastrointestinal oncology 15:2120-2137 PubMed38173425

2024

Hsa_circ_0136666 mediates the antitumor effect of curcumin in colorectal carcinoma by regulating miR-1301-3p.

Applications

Unspecified application

Species

Unspecified reactive species

Shi Chen,Wei Li,Chen-Gong Ning,Feng Wang,Li-Xing Wang,Chen Liao,Feng Sun

Nature communications 14:4677 PubMed37542037

2023

Targeting of SLC25A22 boosts the immunotherapeutic response in KRAS-mutant colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Qiming Zhou,Yao Peng,Fenfen Ji,Huarong Chen,Wei Kang,Lam-Shing Chan,Hongyan Gou,Yufeng Lin,Pingmei Huang,Danyu Chen,Qinyao Wei,Hao Su,Cong Liang,Xiang Zhang,Jun Yu,Chi Chun Wong

Oncology letters 25:225 PubMed37153045

2023

Study on the mechanism of oral administration of tetrandrine during neoadjuvant chemotherapy for colon cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Li,Junmei Li,Feng Yu,Bo Wang,Bing Liu

Journal of dental sciences 18:636-644 PubMed37021271

2023

Myeloid-derived growth factor regulates high glucose-mediated apoptosis of gingival fibroblasts and induce AKT pathway activation and nuclear factor κB pathway inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Linlin Gao,Zhenqiang Li,Wenxiao Chang,Yanyan Liu,Nan Zhang

Frontiers in immunology 14:1117297 PubMed37056767

2023

Gene signature and prediction model of the mitophagy-associated immune microenvironment in renal ischemia-reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Ruo-Yang Chen,Da-Wei Li,Hui Xie,Xiao-Wen Liu,Shao-Yong Zhuang,Hao-Yu Wu,Jia-Jin Wu,Nan Sun,Jun-Wen Qu,Jia-Yi Miao,Chen Zhong,Yu-Hua Huang,Xiao-Dong Yuan,Ming Zhang,Wei-Jie Zhang,Jian-Quan Hou

Cellular and molecular gastroenterology and hepatology 15:463-485 PubMed36309200

2022

DCLK1 Suppresses Tumor-Specific Cytotoxic T Lymphocyte Function Through Recruitment of MDSCs via the CXCL1-CXCR2 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Yan,Jianjian Li,Zeru Xiao,Xiaona Fan,Heshu Liu,Ying Xu,Ruya Sun,Jian Liu,Jiannan Yao,Guangyu An,Yan Shi,Yang Ge

Scientific reports 10:4000 PubMed32132577

2020

Oxidative stress enhanced the transforming growth factor-β2-induced epithelial-mesenchymal transition through chemokine ligand 1 on ARPE-19 cell.

Applications

Unspecified application

Species

Unspecified reactive species

I-Hui Yang,Jong-Jer Lee,Pei-Chang Wu,Hsi-Kung Kuo,Yu-Hsia Kuo,Hsiu-Mei Huang

FEBS open bio 9:2105-2116 PubMed31651102

2019

IL-8 induces transdifferentiation of mature hepatocytes toward the cholangiocyte phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

Tokio Sasaki,Yuji Suzuki,Keisuke Kakisaka,Ting Wang,Kazuyuki Ishida,Akiko Suzuki,Hiroaki Abe,Tamotsu Sugai,Yasuhiro Takikawa

International journal of molecular sciences 20: PubMed30870978

2019

Detection of Inflammation-Related Melanoma Small Extracellular Vesicle (sEV) mRNA Content Using Primary Melanocyte sEVs as a Reference.

Applications

Unspecified application

Species

Unspecified reactive species

Gina T Bardi,Numan Al-Rayan,Jamaal L Richie,Kavitha Yaddanapudi,Joshua L Hood

Oncotarget 8:63901-63910 PubMed28969039

2017

EGFR or HER2 inhibition modulates the tumor microenvironment by suppression of PD-L1 and cytokines release.

Applications

ELISA

Species

Unspecified reactive species

Koung Jin Suh,Ji Hea Sung,Jin Won Kim,Song-Hee Han,Hye Seung Lee,Ahrum Min,Mi Hyun Kang,Ji Eun Kim,Ji-Won Kim,Se Hyun Kim,Jeong-Ok Lee,Yu Jung Kim,Keun-Wook Lee,Jee Hyun Kim,Soo-Mee Bang,Seock-Ah Im,Jong Seok Lee
View all publications
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