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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Human CXCL1 ELISA Kit (GRO alpha) is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human CXCL1 (GRO alpha) in Cerebral Spinal Fluid, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum samples.
Colorimetric
Cerebral Spinal Fluid, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum
Sandwich (quantitative)
Human
2.34 - 150 pg/mL
1h 30m
= 0.064 pg/mL
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.
Growth-regulated alpha protein, C-X-C motif chemokine 1, GRO-alpha(1-73), Melanoma growth stimulatory activity, Neutrophil-activating protein 3, MGSA, NAP-3, MGSA, GROA, SCYB1, GRO1, GRO, CXCL1
Human CXCL1 ELISA Kit (GRO alpha) is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human CXCL1 (GRO alpha) in Cerebral Spinal Fluid, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum samples.
Growth-regulated alpha protein, C-X-C motif chemokine 1, GRO-alpha(1-73), Melanoma growth stimulatory activity, Neutrophil-activating protein 3, MGSA, NAP-3, MGSA, GROA, SCYB1, GRO1, GRO, CXCL1
Colorimetric
Cerebral Spinal Fluid, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum
Sandwich (quantitative)
Human
2.34 - 150 pg/mL
1h 30m
Microplate
= 0.064 pg/mL
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Supernatant | n 3 | mean - | SD - | C.V. 1.9 |
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Supernatant | n 8 | mean - | SD - | C.V. 3.7 |
Sample type | Average % | Range |
---|---|---|
Sample type Cerebral Spinal Fluid | Average % = 96 | Range 93 - 101 % |
Sample type Cell culture supernatant | Average % = 95 | Range 92 - 97 % |
Sample type Serum | Average % = 83 | Range 82 - 85 % |
Sample type Heparin Plasma | Average % = 86 | Range 85 - 87 % |
Sample type Citrate plasma | Average % = 81 | Range 80 - 82 % |
Blue Ice
+4°C
+4°C
+4°C
Human CXCL1 ELISA Kit (ab190805) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CXCL1 protein in cell culture supernatant, cit plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CXCL1 with 0.064 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
CXCL1 (GRO alpha) is a member of the CXC family of chemokines. CXCL1 contains the ELR motif and preferentially chemoattracts neutrophils. Human CXCL1 undergoes post-translational modification into three isoforms called CXCL1(4-73), CXCL1(5-73) and CXCL1(6-73). These isoforms are produced by proteolytic cleavage after secretion from peripheral blood monocytes, and all show higher activity in-vitro than the full-length protein. Two additional CXC proteins, CXCL2 (or GRO beta) and CXCL3 (or GRO gamma), share 90% and 86% amino acid sequence homology, respectively, with CXCL1. Human CXCL1 mRNA is expressed in foreskin fibroblasts, synovial fibroblasts, chondrocytes and osteocytes. Additionally, CXCL1 mRNA has been detected in mammary fibroblasts and epithelial cells, endothelial cells, activated monocytes, macrophages, and neutrophils. CXCL1 is a growth-regulated gene and is overexpressed constitutively in tumorigenic cells. As a result, elevated levels of CXCL1 can be seen in several types of tumors and cancers, including lung cancer and melanoma.
CXCL1 functions as a significant mediator in inflammatory processes. It does not form a complex but acts independently to exert its effects. By attracting neutrophils to sites of injury or infection CXCL1 supports the body's defense mechanisms. This chemokine also influences angiogenesis contributing to the formation of new blood vessels which is essential in wound healing and tissue regeneration.
CXCL1 also known as GRO alpha or KC in certain species is a chemokine with a molecular mass of around 7.8 kDa. This protein is primarily secreted by macrophages neutrophils and endothelial cells. It plays a critical role in the recruitment of neutrophils during inflammation and acts by binding to the CXCR2 receptor. CXCL1 is expressed in many tissues including lungs liver and skin where it regulates the immune response and facilitates tissue repair.
CXCL1 plays a role in both the NF-kB signaling and MAPK signaling pathways. These pathways are pivotal for the regulation of immune and inflammatory responses. CXCL1 acts alongside proteins such as IL-8 another chemokine that also binds to the CXCR2 receptor. Through these pathways CXCL1 influences the activation and migration of immune cells facilitating a rapid response to inflammatory stimuli.
CXCL1 has a significant association with conditions such as chronic obstructive pulmonary disease (COPD) and rheumatoid arthritis. In COPD elevated levels of CXCL1 contribute to persistent inflammation and neutrophil infiltration. In rheumatoid arthritis CXCL1 participates in joint inflammation and damage interacting with proteins like TNF-alpha which amplifies inflammatory responses. Understanding the role of CXCL1 in these conditions could help guide the development of targeted therapies to mitigate inflammation and tissue damage.
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Human CXCL1 (GRO alpha) Standard Curve Comparison.
Standard Curve comparison between human CXCL1 SimpleStep ELISA kit (GRO alpha) and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
Human CXCL1 (GRO alpha) Standard Curve Comparison between new and original ELISA kit.
Standard Curve comparison between new human CXCL1 SimpleStep ELISA kit (GRO alpha) and original ELISA kit. The current SimpleStep ELISA kit shows increased sensitivity.
Example of human CXCL1 (GRO alpha) standard curve in Sample Diluent NS.
The CXCL1 (GRO alpha) standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Specificity of CXCL1 (GRO alpha) signal on unstimulated and stimulated media supernatants.
Human PBMCs were cultured in RPMI supplemented with 10% fetal calf serum, 2 mM L-glutamine. Cells were cultured for 2 days at 37˚C in the presence or absence of 1.5% PHA-M. The concentrations of CXCL1 (GRO alpha) were interpolated from the standard curve in Sample Diluent NS and corrected for sample dilution. The mean CXCL1 concentration was determined to be 5.11 pg/mL in unstimulated PBMC supernatants, and 17533.21 pg/mL in stimulated PBMC supernatants.
Interpolated concentrations of native CXCL1 (GRO alpha) in human PBMC supernatant from cells treated with 1.5% PHA-M for 46 hours.
The concentrations of CXCL1 (GRO alpha) were measured in duplicate and interpolated from the CXCL1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL1 concentration was determined to be 17.53 ng/mL in treated PBMC supernatant.
Interpolated concentrations of human CXCL1 in serum, platelet poor plasma (citrate), platelet poor plasma (heparin), and CSF.
Interpolated concentration of native CXCL1 was measured in duplicate at different sample concentrations. Undiluted samples are 100% serum, 100% platelet poor plasma (citrate), 100% platelet poor plasma (heparin), and 50% CSF. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
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