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Human CXCL1 ELISA Kit (GRO alpha) is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human CXCL1 (GRO alpha) in Cerebral Spinal Fluid, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum samples.


Images

Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

2.34 - 150 pg/mL

Assay time

1h 30m

Sensitivity

= 0.064 pg/mL

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Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.

Alternative names

What's included?

10 x 96 Tests
Components
10X Human CXCL1 Capture Antibody
1 x 6000 µL
10X Human CXCL1 Detector Antibody
1 x 6000 µL
10X Wash Buffer PT (ab206977)
1 x 200 mL
Antibody Diluent 5BI
10 x 6 mL
Human CXCL1 Lyophilized Recombinant Protein (ab9806)
2 x 10 Vial
Plate Seals
10 x 1 Unit
Sample Diluent NS (ab193972)
2 x 250 mL
SimpleStep Pre-Coated 96-Well Microplate (ab206978)
10 x 1 Unit
Stop Solution
1 x 120 mL
TMB Development Solution
1 x 120 mL

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Human CXCL1 ELISA Kit (GRO alpha) is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human CXCL1 (GRO alpha) in Cerebral Spinal Fluid, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum samples.

Alternative names

Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

2.34 - 150 pg/mL

Assay time

1h 30m

Assay Platform

Microplate

Sensitivity

= 0.064 pg/mL

Precision

Intra assay

Sample

Supernatant

n

3

mean

-

SD

-

C.V.

1.9

Inter assay

Sample

Supernatant

n

8

mean

-

SD

-

C.V.

3.7

Recovery

Sample specific recovery

Sample type

Cerebral Spinal Fluid

Average %

= 96

Range

93 - 101 %

Sample type

Cell culture supernatant

Average %

= 95

Range

92 - 97 %

Sample type

Serum

Average %

= 83

Range

82 - 85 %

Sample type

Heparin Plasma

Average %

= 86

Range

85 - 87 %

Sample type

Citrate plasma

Average %

= 81

Range

80 - 82 %

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Human CXCL1 ELISA Kit (ab190805) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CXCL1 protein in cell culture supernatant, cit plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CXCL1 with 0.064 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

CXCL1 (GRO alpha) is a member of the CXC family of chemokines. CXCL1 contains the ELR motif and preferentially chemoattracts neutrophils. Human CXCL1 undergoes post-translational modification into three isoforms called CXCL1(4-73), CXCL1(5-73) and CXCL1(6-73). These isoforms are produced by proteolytic cleavage after secretion from peripheral blood monocytes, and all show higher activity in-vitro than the full-length protein. Two additional CXC proteins, CXCL2 (or GRO beta) and CXCL3 (or GRO gamma), share 90% and 86% amino acid sequence homology, respectively, with CXCL1. Human CXCL1 mRNA is expressed in foreskin fibroblasts, synovial fibroblasts, chondrocytes and osteocytes. Additionally, CXCL1 mRNA has been detected in mammary fibroblasts and epithelial cells, endothelial cells, activated monocytes, macrophages, and neutrophils. CXCL1 is a growth-regulated gene and is overexpressed constitutively in tumorigenic cells. As a result, elevated levels of CXCL1 can be seen in several types of tumors and cancers, including lung cancer and melanoma.

Supplementary info

Biological summary

CXCL1 functions as a significant mediator in inflammatory processes. It does not form a complex but acts independently to exert its effects. By attracting neutrophils to sites of injury or infection CXCL1 supports the body's defense mechanisms. This chemokine also influences angiogenesis contributing to the formation of new blood vessels which is essential in wound healing and tissue regeneration.

Mechanical summary

CXCL1 also known as GRO alpha or KC in certain species is a chemokine with a molecular mass of around 7.8 kDa. This protein is primarily secreted by macrophages neutrophils and endothelial cells. It plays a critical role in the recruitment of neutrophils during inflammation and acts by binding to the CXCR2 receptor. CXCL1 is expressed in many tissues including lungs liver and skin where it regulates the immune response and facilitates tissue repair.

Pathway

CXCL1 plays a role in both the NF-kB signaling and MAPK signaling pathways. These pathways are pivotal for the regulation of immune and inflammatory responses. CXCL1 acts alongside proteins such as IL-8 another chemokine that also binds to the CXCR2 receptor. Through these pathways CXCL1 influences the activation and migration of immune cells facilitating a rapid response to inflammatory stimuli.

Disease

CXCL1 has a significant association with conditions such as chronic obstructive pulmonary disease (COPD) and rheumatoid arthritis. In COPD elevated levels of CXCL1 contribute to persistent inflammation and neutrophil infiltration. In rheumatoid arthritis CXCL1 participates in joint inflammation and damage interacting with proteins like TNF-alpha which amplifies inflammatory responses. Understanding the role of CXCL1 in these conditions could help guide the development of targeted therapies to mitigate inflammation and tissue damage.

Product promise

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Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Sandwich ELISA - Human CXCL1 ELISA Kit (GRO alpha) (ab190805), expandable thumbnail

    Sandwich ELISA - Human CXCL1 ELISA Kit (GRO alpha) (ab190805)

    Human CXCL1 (GRO alpha) Standard Curve Comparison.

    Standard Curve comparison between human CXCL1 SimpleStep ELISA kit (GRO alpha) and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

  • Sandwich ELISA - Human CXCL1 ELISA Kit (GRO alpha) (ab190805), expandable thumbnail

    Sandwich ELISA - Human CXCL1 ELISA Kit (GRO alpha) (ab190805)

    Human CXCL1 (GRO alpha) Standard Curve Comparison between new and original ELISA kit.

    Standard Curve comparison between new human CXCL1 SimpleStep ELISA kit (GRO alpha) and original ELISA kit. The current SimpleStep ELISA kit shows increased sensitivity.

  • Sandwich ELISA - Human CXCL1 ELISA Kit (GRO alpha) (ab190805), expandable thumbnail

    Sandwich ELISA - Human CXCL1 ELISA Kit (GRO alpha) (ab190805)

    Example of human CXCL1 (GRO alpha) standard curve in Sample Diluent NS.

    The CXCL1 (GRO alpha) standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • Sandwich ELISA - Human CXCL1 ELISA Kit (GRO alpha) (ab190805), expandable thumbnail

    Sandwich ELISA - Human CXCL1 ELISA Kit (GRO alpha) (ab190805)

    Specificity of CXCL1 (GRO alpha) signal on unstimulated and stimulated media supernatants.

    Human PBMCs were cultured in RPMI supplemented with 10% fetal calf serum, 2 mM L-glutamine. Cells were cultured for 2 days at 37˚C in the presence or absence of 1.5% PHA-M. The concentrations of CXCL1 (GRO alpha) were interpolated from the standard curve in Sample Diluent NS and corrected for sample dilution. The mean CXCL1 concentration was determined to be 5.11 pg/mL in unstimulated PBMC supernatants, and 17533.21 pg/mL in stimulated PBMC supernatants.

  • Sandwich ELISA - Human CXCL1 ELISA Kit (GRO alpha) (ab190805), expandable thumbnail

    Sandwich ELISA - Human CXCL1 ELISA Kit (GRO alpha) (ab190805)

    Interpolated concentrations of native CXCL1 (GRO alpha) in human PBMC supernatant from cells treated with 1.5% PHA-M for 46 hours.

    The concentrations of CXCL1 (GRO alpha) were measured in duplicate and interpolated from the CXCL1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL1 concentration was determined to be 17.53 ng/mL in treated PBMC supernatant.

  • Sandwich ELISA - Human CXCL1 ELISA Kit (GRO alpha) (ab190805), expandable thumbnail

    Sandwich ELISA - Human CXCL1 ELISA Kit (GRO alpha) (ab190805)

    Interpolated concentrations of human CXCL1 in serum, platelet poor plasma (citrate), platelet poor plasma (heparin), and CSF.

    Interpolated concentration of native CXCL1 was measured in duplicate at different sample concentrations. Undiluted samples are 100% serum, 100% platelet poor plasma (citrate), 100% platelet poor plasma (heparin), and 50% CSF. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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