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AB269370

Human CXCL13 ELISA Kit (BCA-1)

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(2 Publications)

Human CXCL13 ELISA Kit (BCA-1) is a single-wash 90-min Simplestep used to quantify Human CXCL13 (BCA-1) with a sensitivity of 1 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

BCA1, BLC, SCYB13, CXCL13, C-X-C motif chemokine 13, Angie, B cell-attracting chemokine 1, B lymphocyte chemoattractant, CXC chemokine BLC, Small-inducible cytokine B13, BCA-1

7 Images
ELISA - Human CXCL13 ELISA Kit (BCA-1) (AB269370)
  • ELISA

Supplier Data

ELISA - Human CXCL13 ELISA Kit (BCA-1) (AB269370)
Sandwich ELISA - Human CXCL13 ELISA Kit (BCA-1) (AB269370)
  • sELISA

Supplier Data

Sandwich ELISA - Human CXCL13 ELISA Kit (BCA-1) (AB269370)

Human CXCL13 standard curve comparison.

Standard Curve comparison between human CXCL13 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

Sandwich ELISA - Human CXCL13 ELISA Kit (BCA-1) (AB269370)
  • sELISA

Supplier Data

Sandwich ELISA - Human CXCL13 ELISA Kit (BCA-1) (AB269370)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean of detectable CXCL13 concentration was determined to be 12.7 pg/mL with a range of undetectable to 18.3 pg/mL.

Sandwich ELISA - Human CXCL13 ELISA Kit (BCA-1) (AB269370)
  • sELISA

Supplier Data

Sandwich ELISA - Human CXCL13 ELISA Kit (BCA-1) (AB269370)

Human CXCL13 standard curve comparison.

Standard Curve comparison between the original human MMP3 SimpleStep ELISA kit (ab179881) and current human MMP3 SimpleStep ELISA kit (ab269370).

Sandwich ELISA - Human CXCL13 ELISA Kit (BCA-1) (AB269370)
  • sELISA

Supplier Data

Sandwich ELISA - Human CXCL13 ELISA Kit (BCA-1) (AB269370)

Interpolated concentrations of native CXCL13 in human serum, plasma-citrate, plasma-EDTA and plasma-heparin samples.

The concentrations of CXCL13 were measured in duplicates in neat samples and interpolated from the CXCL13 standard curves. The interpolated values are plotted (mean +/- SD, n=2). The mean CXCL13 concentration was determined to be 13 pg/mL in serum, 12.6 pg/mL in plasma-citrate, 13.1 pg/mL in plasma-EDTA and 10 pg/mL in plasma-heparin.

Sandwich ELISA - Human CXCL13 ELISA Kit (BCA-1) (AB269370)
  • sELISA

Supplier Data

Sandwich ELISA - Human CXCL13 ELISA Kit (BCA-1) (AB269370)

Interpolated concentrations of native CXCL13 in human THP-1 supernatant treated or untreated with Hu-IFN-r, LPS and PBMC cell culture supernatant treated with or without 1.5% PHA-M for 36 hours.

The concentrations of CXCL13 were measured in duplicates, interpolated from the CXCL13 standard curves and corrected for sample dilution. Undiluted samples are as follows : THP-1 treated 25%, THP-1 untreated 100%, PBMC treated 50% and PBMC untreated 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL13 concentration was determined to be 1,292 pg/mL in neat THP-1 treated supernatant, undetectable in untreated THP-1 supernatant, 337 pg/mL in treated neat PBMC supernatant and 25 pg/mL in untreated neat PBMC supernatant.

Sandwich ELISA - Human CXCL13 ELISA Kit (BCA-1) (AB269370)
  • sELISA

Supplier Data

Sandwich ELISA - Human CXCL13 ELISA Kit (BCA-1) (AB269370)

Example of human CXCL13 standard curve in Sample Diluent NS.

The CXCL13 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Saliva, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 1 pg/mL

Range

6.25 - 400 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human CXCL13 ELISA Kit (BCA-1) ab269370 is a rapid single-wash 90-min Sandwich ELISA to measure Human CXCL13 (BCA-1)in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, saliva, serum. This SimpleStep sensitivity is 1 pg/mL.

How the assay works

Human CXCL13 (BCA-1)SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human CXCL13 ELISA Kit (BCA-1) ab269370 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

CXCL13 (C-X-C motif chemokine 13), also known as BCA-1(B-lymphocyte chemoattractant/B cell-attracting chemokine 1) is a member of the CXC chemokine superfamily. CXCL13 is produced by stromal cells from the liver, spleen, lymph nodes and the gut. It binds to the CXCR5 receptor in lymphoid follicles controlling the homing signal of B cells and subsets of T cells to the lymphoid nodes, compartmentalizing lymphocytes and antigen presenting cells within the follicles of lymphoid tissues. CXCL13 is also required for the embryonic development of most of lymph nodes and Peyer's patches. CXCL13-/- as well as CXCR5-/- mice lack secondary lymphoid structures and show a disrupted splenic architecture.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "0.9" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.5" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "111 - 123 %", "average": "= 117" }, { "sample": "Serum", "range": "88 - 94 %", "average": "= 90" }, { "sample": "EDTA Plasma", "range": "88 - 94 %", "average": "= 91" }, { "sample": "Saliva", "range": "78 - 97 %", "average": "= 87" }, { "sample": "Heparin Plasma", "range": "75 - 85 %", "average": "= 80" }, { "sample": "Citrate plasma", "range": "79 - 85 %", "average": "= 82" }, { "sample": "Cell culture media", "range": "78 - 88 %", "average": "= 84" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab269370-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB269370-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB269370-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB269370-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB269370-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB269370-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CXCL13 (BCA-1) Capture Antibody", "number":"AB269370-CMP01", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB269370-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human CXCL13 (BCA-1) Lyophilized Recombinant Protein", "number":"AB269370-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CXCL13 (BCA-1) Detector Antibody", "number":"AB269370-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB269370-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CXCL13 also known as B cell-attracting chemokine 1 (BCA-1) is a chemokine with a molecular weight of approximately 11 kDa. This protein plays an important mechanical role in the immune system by guiding the movement of B cells via chemotaxis. CXCL13 expression occurs mainly in secondary lymphoid tissues such as the spleen lymph nodes and mucosa-associated lymphoid tissue. Its expression is also noted in certain non-lymphoid tissues under inflammatory conditions.
Biological function summary

In the context of immune response and lymphoid tissue organization CXCL13 attracts B cells by interacting with the chemokine receptor CXCR5. These interactions support the formation of B-cell follicles essential for efficient immune responses. CXCL13 is not known to be part of a larger protein complex but instead works through its specific receptor-ligand interactions. Its role in guiding cell migration is vital for the development of secondary lymphoid structures.

Pathways

CXCL13 is significantly involved in the B-cell receptor signaling pathway. The chemokine facilitates the homing of B cells to follicles an important step in the adaptive immune response. CXCL13 closely associates with CXCR5 its specific receptor playing a part in establishing the spatial organization of lymphoid tissues. Additionally the chemokine influences the germinal center reaction an integral part of the immune pathway for antibody maturation.

CXCL13 levels have been linked to autoimmune diseases like rheumatoid arthritis and certain types of lymphomas. In rheumatoid arthritis increased CXCL13 expression correlates with disease severity related to its role in recruiting B cells that contribute to chronic inflammation. In lymphomas particularly those involving B cells CXCL13 supports the growth and survival of malignant cells. Its association with disease states often involves changes in CXCR5 expression or function contributing to disrupted B-cell localization and abnormal immune responses.
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Product protocols

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Target data

Chemotactic for B-lymphocytes but not for T-lymphocytes, monocytes and neutrophils. Does not induce calcium release in B-lymphocytes. Binds to BLR1/CXCR5.
See full target information CXCL13
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:6959 PubMed37907518

2023

Peripheral helper-T-cell-derived CXCL13 is a crucial pathogenic factor in idiopathic multicentric Castleman disease.

Applications

Unspecified application

Species

Unspecified reactive species

Takuya Harada,Yoshikane Kikushige,Toshihiro Miyamoto,Kazuko Uno,Hiroaki Niiro,Atsushi Kawakami,Tomohiro Koga,Koichi Akashi,Kazuyuki Yoshizaki

Journal of immunology research 2022:9003902 PubMed36339942

2022

The Regulation between CD4CXCR5 Follicular Helper T (Tfh) Cells and CD19CD24CD38 Regulatory B (Breg) Cells in Gastric Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Zhang,Jun Wu,Haibo Zhang,Chen Wu
View all publications
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