Human CXCL16 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human CXCL16 in Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.
Colorimetric
Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
Sandwich (quantitative)
Human
31.25 - 2000 pg/mL
1h 30m
= 2 pg/mL
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Acts as a scavenger receptor on macrophages, which specifically binds to OxLDL (oxidized low density lipoprotein), suggesting that it may be involved in pathophysiology such as atherogenesis (By similarity). Induces a strong chemotactic response. Induces calcium mobilization. Binds to CXCR6/Bonzo.
SCYB16, SRPSOX, UNQ2759/PRO6714, CXCL16, C-X-C motif chemokine 16, Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein, Small-inducible cytokine B16, Transmembrane chemokine CXCL16, SR-PSOX
Human CXCL16 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human CXCL16 in Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.
Colorimetric
Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
Sandwich (quantitative)
Human
31.25 - 2000 pg/mL
1h 30m
Microplate
= 2 pg/mL
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Human Serum | n 5 | mean - | SD - | C.V. 1.5 |
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Human Serum | n 3 | mean - | SD - | C.V. 5.6 |
Sample type | Average % | Range |
---|---|---|
Sample type Serum | Average % = 81.2 | Range 79.5 - 82.5 % |
Sample type EDTA Plasma | Average % = 87.9 | Range 80.8 - 91.8 % |
Sample type Heparin Plasma | Average % = 87.1 | Range 73.5 - 101.3 % |
Sample type Citrate plasma | Average % = 87 | Range 85.9 - 87.8 % |
Sample type Cell culture media | Average % = 108 | Range 104.3 - 114.5 % |
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Human CXCL16 ELISA Kit (ab187397) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CXCL16 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CXCL16 with 2 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
CXCL16 is a member of the chemokine superfamily. CXCL16 may actsas a scavenger receptor on macrophages, which specifically binds to OxLDL (oxidized low density lipoprotein), suggesting that it may be involved in pathophysiology such as atherogenesis. CXCL16 induces a strong chemotactic response. CXCL16 may have a role in leukocytes migration. CXCL16 induces calcium mobilization.
This supplementary information is collated from multiple sources and compiled automatically.
CXCL16 also known as SR-PSOX is a chemokine that functions primarily in cellular signaling. It weighs approximately 32 kDa and exists in both membrane-bound and soluble forms. Primarily found in the spleen liver lungs and kidneys CXCL16 gets highly expressed on the surface of antigen-presenting cells such as dendritic cells and macrophages. This expression enables CXCL16 to perform its role in cell migration and adhesion by binding to its receptor CXCR6.
CXCL16 plays a critical role in the immune response by mediating leukocyte migration. It acts as a chemoattractant for T cells especially those expressing the CXCR6 receptor facilitating their movement to sites of inflammation or injury. CXCL16 is not known to be part of a larger protein complex but interacts independently to moderate immune functions. It also participates in scavenging oxidized low-density lipoproteins linking it to processes in atherogenesis.
CXCL16 is an important player in the inflammatory and immune pathways. It is involved in the NF-kB signaling pathway which regulates inflammatory responses and the chemokine signaling pathway. Through these pathways CXCL16 closely interacts with other chemokines and cytokines such as CCR5 and IL-8 helping coordinate immune surveillance and inflammatory cascade.
CXCL16 is heavily implicated in atherosclerosis and rheumatoid arthritis. In atherosclerosis CXCL16 contributes to the accumulation of macrophages and foam cell formation in plaques. This connection associates it with inflammatory molecules like TNF-alpha. In rheumatoid arthritis CXCL16 expression is increased in synovial tissues contributing to tissue damage and inflammation potentially linked through interactions with other inflammatory mediators such as IL-6.
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Example of CXCL16 standard curve.
Background-subtracted data values (mean +/- SD) are graphed.
Titration of Human serum and plasma within the working range of the assay.
Background-subtracted data values (mean +/- SD, n = 2) are graphed.
Titration of HepG2 cell culture supernatant within the working range of the assay.
Background-subtracted data values (mean +/- SD, n = 2) are graphed.
CXCL16 concentrations in 10 individual Human serum donors.
8 X diluted serum samples from 10 apparently healthy male donors were measured using this kit. Interpolated data values corrected for sample dilution are graphed in ng of CXCL16 per mL of serum (mean +/- SD, n=3). The mean of CXCL16 concentration of these ten individual serum samples was determined to be 3.26 ng /mL with a range of 2.58 – 4.08 ng /mL.
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