AB187397

Human CXCL16 ELISA Kit

SimpleStep
What is this?

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(1 Publication)

Human CXCL16 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CXCL16 with a sensitivity of 2 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

SCYB16, SRPSOX, UNQ2759/PRO6714, CXCL16, C-X-C motif chemokine 16, Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein, Small-inducible cytokine B16, Transmembrane chemokine CXCL16, SR-PSOX

4 Images

Sandwich ELISA - Human CXCL16 ELISA Kit (AB187397)

sELISA

Supplier Data

Sandwich ELISA - Human CXCL16 ELISA Kit (AB187397)

CXCL16 concentrations in 10 individual Human serum donors.

8 X diluted serum samples from 10 apparently healthy male donors were measured using this kit. Interpolated data values corrected for sample dilution are graphed in ng of CXCL16 per mL of serum (mean +/- SD, n=3). The mean of CXCL16 concentration of these ten individual serum samples was determined to be 3.26 ng /mL with a range of 2.58 – 4.08 ng /mL.

sELISA

Supplier Data

Sandwich ELISA - Human CXCL16 ELISA Kit (AB187397)

Titration of HepG2 cell culture supernatant within the working range of the assay.

Background-subtracted data values (mean +/- SD, n = 2) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human CXCL16 ELISA Kit (AB187397)

Example of CXCL16 standard curve.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human CXCL16 ELISA Kit (AB187397)

Titration of Human serum and plasma within the working range of the assay.

Background-subtracted data values (mean +/- SD, n = 2) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 2 pg/mL

Range

31.25 - 2000 pg/mL

Assay time

1h 30m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human CXCL16 ELISA Kit (ab187397) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CXCL16 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CXCL16 with 2 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

CXCL16 is a member of the chemokine superfamily. CXCL16 may actsas a scavenger receptor on macrophages, which specifically binds to OxLDL (oxidized low density lipoprotein), suggesting that it may be involved in pathophysiology such as atherogenesis. CXCL16 induces a strong chemotactic response. CXCL16 may have a role in leukocytes migration. CXCL16 induces calcium mobilization.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Human Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.6" }, { "reproducibilityType": "Intra", "sample": "Human Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.5" } ]

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Recovery

[ { "sample": "Serum", "range": "79.5 - 82.5 %", "average": "= 81.2" }, { "sample": "EDTA Plasma", "range": "80.8 - 91.8 %", "average": "= 87.9" }, { "sample": "Heparin Plasma", "range": "73.5 - 101.3 %", "average": "= 87.1" }, { "sample": "Citrate plasma", "range": "85.9 - 87.8 %", "average": "= 87" }, { "sample": "Cell culture media", "range": "104.3 - 114.5 %", "average": "= 108" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CXCL16 also known as SR-PSOX is a chemokine that functions primarily in cellular signaling. It weighs approximately 32 kDa and exists in both membrane-bound and soluble forms. Primarily found in the spleen liver lungs and kidneys CXCL16 gets highly expressed on the surface of antigen-presenting cells such as dendritic cells and macrophages. This expression enables CXCL16 to perform its role in cell migration and adhesion by binding to its receptor CXCR6.

Biological function summary

CXCL16 plays a critical role in the immune response by mediating leukocyte migration. It acts as a chemoattractant for T cells especially those expressing the CXCR6 receptor facilitating their movement to sites of inflammation or injury. CXCL16 is not known to be part of a larger protein complex but interacts independently to moderate immune functions. It also participates in scavenging oxidized low-density lipoproteins linking it to processes in atherogenesis.

Pathways

CXCL16 is an important player in the inflammatory and immune pathways. It is involved in the NF-kB signaling pathway which regulates inflammatory responses and the chemokine signaling pathway. Through these pathways CXCL16 closely interacts with other chemokines and cytokines such as CCR5 and IL-8 helping coordinate immune surveillance and inflammatory cascade.

CXCL16 is heavily implicated in atherosclerosis and rheumatoid arthritis. In atherosclerosis CXCL16 contributes to the accumulation of macrophages and foam cell formation in plaques. This connection associates it with inflammatory molecules like TNF-alpha. In rheumatoid arthritis CXCL16 expression is increased in synovial tissues contributing to tissue damage and inflammation potentially linked through interactions with other inflammatory mediators such as IL-6.

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Product protocols

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Target data

Acts as a scavenger receptor on macrophages, which specifically binds to OxLDL (oxidized low density lipoprotein), suggesting that it may be involved in pathophysiology such as atherogenesis (By similarity). Induces a strong chemotactic response. Induces calcium mobilization. Binds to CXCR6/Bonzo.

See full target information CXCL16

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Immunity 57:1306-1323.e8 PubMed38815582

2024

Intestinal CXCR6 ILC3s migrate to the kidney and exacerbate renal fibrosis via IL-23 receptor signaling enhanced by PD-1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Zhou Liang,Ziwen Tang,Changjian Zhu,Feng Li,Shuaijiabin Chen,Xu Han,Ruilin Zheng,Xinrong Hu,Ruoni Lin,Qiaoqiao Pei,Changjun Yin,Ji Wang,Ce Tang,Nan Cao,Jincun Zhao,Rong Wang,Xiaoyan Li,Ning Luo,Qiong Wen,Jianwen Yu,Jianbo Li,Xi Xia,Xunhua Zheng,Xin Wang,Naya Huang,Zhong Zhong,Chengqiang Mo,Peisong Chen,Yating Wang,Jinjin Fan,Yun Guo,Haojie Zhong,Jiaqi Liu,Zhenwei Peng,Haiping Mao,Guo-Ping Shi,Joseph V Bonventre,Wei Chen,Yi Zhou

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human CXCL16 ELISA Kit

Sandwich ELISA - Human CXCL16 ELISA Kit (AB187397)

Sandwich ELISA - Human CXCL16 ELISA Kit (AB187397)

Sandwich ELISA - Human CXCL16 ELISA Kit (AB187397)

Sandwich ELISA - Human CXCL16 ELISA Kit (AB187397)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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