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Human CXCL16 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human CXCL16 in Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.

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Images

Sandwich ELISA - Human CXCL16 ELISA Kit (AB187397), expandable thumbnail
  • Sandwich ELISA - Human CXCL16 ELISA Kit (AB187397), expandable thumbnail
  • Sandwich ELISA - Human CXCL16 ELISA Kit (AB187397), expandable thumbnail
  • Sandwich ELISA - Human CXCL16 ELISA Kit (AB187397), expandable thumbnail

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

31.25 - 2000 pg/mL

Assay time

1h 30m

Sensitivity

= 2 pg/mL

Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Acts as a scavenger receptor on macrophages, which specifically binds to OxLDL (oxidized low density lipoprotein), suggesting that it may be involved in pathophysiology such as atherogenesis (By similarity). Induces a strong chemotactic response. Induces calcium mobilization. Binds to CXCR6/Bonzo.

Alternative names

What's included?

1 x 96 Tests
Components
10X Human CXCL16 Capture Antibody
1 x 600 µL
10X Human CXCL16 Detector Antibody
1 x 600 µL
10X Wash Buffer PT (ab206977)
1 x 20 mL
Antibody Diluent CPI2
1 x 6 mL
Human CXCL16 Lyophilized Recombinant Protein
2 x 1 Vial
Plate Seals
1 x 1 Unit
Sample Diluent NS (ab193972)
1 x 50 mL
SimpleStep Pre-Coated 96-Well Microplate (ab206978)
1 x 1 Unit
Stop Solution
1 x 12 mL
TMB Development Solution
1 x 12 mL

Recommended products

Human CXCL16 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human CXCL16 in Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

31.25 - 2000 pg/mL

Assay time

1h 30m

Assay Platform

Microplate

Sensitivity

= 2 pg/mL

Precision

Intra assay

Sample

Human Serum

n
5
mean
-
SD
-
C.V.

1.5

Inter assay

Sample

Human Serum

n
3
mean
-
SD
-
C.V.

5.6

Recovery

Sample specific recovery

Sample type

Serum

Average %

= 81.2

Range

79.5 - 82.5 %

Sample type

EDTA Plasma

Average %

= 87.9

Range

80.8 - 91.8 %

Sample type

Heparin Plasma

Average %

= 87.1

Range

73.5 - 101.3 %

Sample type

Citrate plasma

Average %

= 87

Range

85.9 - 87.8 %

Sample type

Cell culture media

Average %

= 108

Range

104.3 - 114.5 %

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Human CXCL16 ELISA Kit (ab187397) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CXCL16 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CXCL16 with 2 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

CXCL16 is a member of the chemokine superfamily. CXCL16 may actsas a scavenger receptor on macrophages, which specifically binds to OxLDL (oxidized low density lipoprotein), suggesting that it may be involved in pathophysiology such as atherogenesis. CXCL16 induces a strong chemotactic response. CXCL16 may have a role in leukocytes migration. CXCL16 induces calcium mobilization.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CXCL16 also known as SR-PSOX is a chemokine that functions primarily in cellular signaling. It weighs approximately 32 kDa and exists in both membrane-bound and soluble forms. Primarily found in the spleen liver lungs and kidneys CXCL16 gets highly expressed on the surface of antigen-presenting cells such as dendritic cells and macrophages. This expression enables CXCL16 to perform its role in cell migration and adhesion by binding to its receptor CXCR6.

Biological function summary

CXCL16 plays a critical role in the immune response by mediating leukocyte migration. It acts as a chemoattractant for T cells especially those expressing the CXCR6 receptor facilitating their movement to sites of inflammation or injury. CXCL16 is not known to be part of a larger protein complex but interacts independently to moderate immune functions. It also participates in scavenging oxidized low-density lipoproteins linking it to processes in atherogenesis.

Pathways

CXCL16 is an important player in the inflammatory and immune pathways. It is involved in the NF-kB signaling pathway which regulates inflammatory responses and the chemokine signaling pathway. Through these pathways CXCL16 closely interacts with other chemokines and cytokines such as CCR5 and IL-8 helping coordinate immune surveillance and inflammatory cascade.

Associated diseases and disorders

CXCL16 is heavily implicated in atherosclerosis and rheumatoid arthritis. In atherosclerosis CXCL16 contributes to the accumulation of macrophages and foam cell formation in plaques. This connection associates it with inflammatory molecules like TNF-alpha. In rheumatoid arthritis CXCL16 expression is increased in synovial tissues contributing to tissue damage and inflammation potentially linked through interactions with other inflammatory mediators such as IL-6.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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4 product images

  • Sandwich ELISA - Human CXCL16 ELISA Kit (ab187397), expandable thumbnail

    Sandwich ELISA - Human CXCL16 ELISA Kit (ab187397)

    Example of CXCL16 standard curve.

    Background-subtracted data values (mean +/- SD) are graphed.

  • Sandwich ELISA - Human CXCL16 ELISA Kit (ab187397), expandable thumbnail

    Sandwich ELISA - Human CXCL16 ELISA Kit (ab187397)

    Titration of Human serum and plasma within the working range of the assay.

    Background-subtracted data values (mean +/- SD, n = 2) are graphed.

  • Sandwich ELISA - Human CXCL16 ELISA Kit (ab187397), expandable thumbnail

    Sandwich ELISA - Human CXCL16 ELISA Kit (ab187397)

    Titration of HepG2 cell culture supernatant within the working range of the assay.

    Background-subtracted data values (mean +/- SD, n = 2) are graphed.

  • Sandwich ELISA - Human CXCL16 ELISA Kit (ab187397), expandable thumbnail

    Sandwich ELISA - Human CXCL16 ELISA Kit (ab187397)

    CXCL16 concentrations in 10 individual Human serum donors.

    8 X diluted serum samples from 10 apparently healthy male donors were measured using this kit. Interpolated data values corrected for sample dilution are graphed in ng of CXCL16 per mL of serum (mean +/- SD, n=3). The mean of CXCL16 concentration of these ten individual serum samples was determined to be 3.26 ng /mL with a range of 2.58 – 4.08 ng /mL.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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