AB323736

Human CXCL16 ELISA Kit

Recombinant
SimpleStep
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Human CXCL16 ELISA Kit is a Sandwich ELISA for the measurement of Human CXCL16 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

SCYB16, SRPSOX, UNQ2759/PRO6714, CXCL16, C-X-C motif chemokine 16, Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein, Small-inducible cytokine B16, Transmembrane chemokine CXCL16, SR-PSOX

8 Images

Sandwich ELISA - Human CXCL16 ELISA Kit (AB323736)

sELISA

Supplier Data

Sandwich ELISA - Human CXCL16 ELISA Kit (AB323736)

Example of human CXCL16 standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human CXCL16 ELISA Kit (AB323736)

Serum of ten individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean CXCL16 concentration was determined to be 7,188 pg/mL with a range of 5,437 – 10,011 pg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human CXCL16 ELISA Kit (AB323736)

Standard curve comparison between Human CXCL16 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. The SimpleStep ELISA kit shows increased sensitivity.

sELISA

Supplier Data

Sandwich ELISA - Human CXCL16 ELISA Kit (AB323736)

Interpolated concentration of native CXCL16 was measured in duplicate at different sample concentrations. Undiluted samples are 5% serum, plasma (citrate), plasma (EDTA), plasma (heparin). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human CXCL16 ELISA Kit (AB323736)

Interpolated concentration of native CXCL16 was measured in duplicate at different sample concentrations. Undiluted samples are 12.5% HepG2 cell culture supernatant and PBMC cell culture supernatant. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human CXCL16 ELISA Kit (AB323736)

Example of human CXCL16 standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human CXCL16 ELISA Kit (AB323736)

Interpolated concentration of native CXCL16 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 5% serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human CXCL16 ELISA Kit (AB323736)

Standard curve comparison between the original Human CXCL16 SimpleStep ELISA (ab187397) and current Human CXCL16 SimpleStep ELISA.

Key facts

Detection method

Colorimetric

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 3.668 pg/mL

Range

14.063 - 900 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human CXCL16 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CXCL16 protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture supernatant. Quantitate Human CXCL16 with 3.668 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 12.3" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4.7" } ]

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Recovery

[ { "sample": "Serum", "range": "96 - 113 %", "average": "= 106" }, { "sample": "Citrate plasma", "range": "91 - 105 %", "average": "= 100" }, { "sample": "EDTA Plasma", "range": "91 - 98 %", "average": "= 95" }, { "sample": "Heparin Plasma", "range": "95 - 113 %", "average": "= 105" }, { "sample": "Cell culture supernatant", "range": "96 - 113 %", "average": "= 102" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Acts as a scavenger receptor on macrophages, which specifically binds to OxLDL (oxidized low density lipoprotein), suggesting that it may be involved in pathophysiology such as atherogenesis (By similarity). Induces a strong chemotactic response. Induces calcium mobilization. Binds to CXCR6/Bonzo.

See full target information CXCL16

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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