Human CXCL3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CXCL3 with a sensitivity of 1.1 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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Publications
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- Nature communications 14:46772023Targeting of SLC25A22 boosts the immunotherapeutic response in KRAS-mutant colorectal cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQiming Zhou,Yao Peng,Fenfen Ji,Huarong Chen,Wei Kang,Lam-Shing Chan,Hongyan Gou,Yufeng Lin,Pingmei Huang,Danyu Chen,Qinyao Wei,Hao Su,Cong Liang,Xiang Zhang,Jun Yu,Chi Chun WongPubMed 37542037
- Oncology letters 25:2252023Study on the mechanism of oral administration of tetrandrine during neoadjuvant chemotherapy for colon cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDan Li,Junmei Li,Feng Yu,Bo Wang,Bing LiuPubMed 37153045
- British journal of cancer 123:459-4702020Omental adipocytes promote peritoneal metastasis of gastric cancer through the CXCL2-VEGFA axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMakoto Natsume et. al.PubMed 32439934
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 1.56 - 100 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 1.1 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Ligand for CXCR2 (By similarity). Has chemotactic activity for neutrophils. May play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. In vitro, the processed form GRO-gamma(5-73) shows a fivefold higher chemotactic activity for neutrophilic granulocytes.
Alternative names
GRO3, GROG, SCYB3, CXCL3, C-X-C motif chemokine 3, GRO-gamma(1-73), Growth-regulated protein gamma, Macrophage inflammatory protein 2-beta, GRO-gamma, MIP2-beta
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Recommended products
Human CXCL3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CXCL3 with a sensitivity of 1.1 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 1.56 - 100 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 1.1 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Protein | n 6 | mean - | SD - | C.V. 2.2 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 97 | Range 91 - 103 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human CXCL3 ELISA Kit (ab234574) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CXCL3 protein in serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CXCL3 with 1.1 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GRO gamma also known as CXCL3 is a chemokine with a molecular weight of approximately 7.9 kDa. It is a small protein belonging to the CXC chemokine family. GRO gamma is expressed in numerous tissues including epithelial cells fibroblasts and several leukocytes. This protein plays a significant role in chemotactic signaling drawing neutrophils to sites of inflammation and infection acting as both a signal and effector molecule in immune response. Researchers often use CXCL3 ELISA kits and GRO protein assays to quantify its expression levels in biological samples.
- Biological function summary
GRO gamma functions as a chemokine that guides neutrophils by binding to its receptor CXCR2. It participates in inflammatory processes and is produced in response to stimuli such as bacterial products and pro-inflammatory cytokines. GRO gamma together with its close family members forms a network facilitating the immune cell recruitment and activation at infection sites. Also this chemokine is included in larger chemokine complexes influencing not just immune cells but also modulating angiogenesis and cellular matrix restructuring.
- Pathways
GRO gamma significantly contributes to the chemokine signaling pathway and the NF-kB signaling pathway. In these pathways it interacts tightly with proteins like CXCR2 which is essential for the signaling cascade that results in immune cell migration. It also engages with the MAPK pathways impacting cell proliferation and apoptosis. These involvements make GRO gamma vital for orchestrating the inflammation response in humans.
- Associated diseases and disorders
GRO gamma is linked to chronic inflammatory conditions and cancer. Elevated levels of GRO gamma have been observed in rheumatoid arthritis where its interaction with CXCR2 exacerbates inflammation and tissue damage. In cancer particularly in colorectal cancer GRO gamma plays a role in tumor progression by promoting angiogenesis and cell proliferation interacting with VEGF and other cytokines. Understanding its functions and relationships opens potential avenues for therapeutic interventions targeting chronic diseases and cancer.
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2 product images
Sandwich ELISA - Human CXCL3 ELISA Kit (ab234574)
Example of human CXCL3 standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human CXCL3 ELISA Kit (ab234574)
Interpolated concentrations of native CXCL3 in human serum.
The concentrations of CXCL3 were measured in duplicates, interpolated from the CXCL3 standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL3 concentration was determined to be 200.4 pg/mL in serum.
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