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AB234574

Human CXCL3 ELISA Kit

5

(1 Review)

|

(3 Publications)

Human CXCL3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CXCL3 with a sensitivity of 1.1 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

GRO3, GROG, SCYB3, CXCL3, C-X-C motif chemokine 3, GRO-gamma(1-73), Growth-regulated protein gamma, Macrophage inflammatory protein 2-beta, GRO-gamma, MIP2-beta

3 Images
ELISA - Human CXCL3 ELISA Kit (AB234574)
  • ELISA

Supplier Data

ELISA - Human CXCL3 ELISA Kit (AB234574)
Sandwich ELISA - Human CXCL3 ELISA Kit (AB234574)
  • sELISA

Supplier Data

Sandwich ELISA - Human CXCL3 ELISA Kit (AB234574)

Interpolated concentrations of native CXCL3 in human serum.

The concentrations of CXCL3 were measured in duplicates, interpolated from the CXCL3 standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL3 concentration was determined to be 200.4 pg/mL in serum.

Sandwich ELISA - Human CXCL3 ELISA Kit (AB234574)
  • sELISA

Supplier Data

Sandwich ELISA - Human CXCL3 ELISA Kit (AB234574)

Example of human CXCL3 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 1.1 pg/mL

Range

1.56 - 100 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human CXCL3 ELISA Kit ab234574 is a rapid single-wash 90-min Sandwich ELISA to measure Human CXCL3 in serum. This SimpleStep sensitivity is 1.1 pg/mL.

How the assay works

Human CXCL3 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human CXCL3 ELISA Kit ab234574 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-GRO gamma antibody [EPR20154-21] - BSA and Azide free (Capture) ab245032
- Anti-GRO gamma antibody [EPR20154-30] - BSA and Azide free (Detector) ab245032
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Precision

[ { "reproducibilityType": "Intra", "sample": "Protein", "replicates": 6, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.2" } ]
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Recovery

[ { "sample": "Serum", "range": "91 - 103 %", "average": "= 97" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab234574-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB234574-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB234574-CMP10", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB234574-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CXCL3 Capture Antibody", "number":"AB234574-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human CXCL3 Lyophilized Recombinant Protein", "number":"AB234574-CMP08", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB234574-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB234574-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB234574-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB234574-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CXCL3 Detector Antibody", "number":"AB234574-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GRO gamma also known as CXCL3 is a chemokine with a molecular weight of approximately 7.9 kDa. It is a small protein belonging to the CXC chemokine family. GRO gamma is expressed in numerous tissues including epithelial cells fibroblasts and several leukocytes. This protein plays a significant role in chemotactic signaling drawing neutrophils to sites of inflammation and infection acting as both a signal and effector molecule in immune response. Researchers often use CXCL3 ELISA kits and GRO protein assays to quantify its expression levels in biological samples.
Biological function summary

GRO gamma functions as a chemokine that guides neutrophils by binding to its receptor CXCR2. It participates in inflammatory processes and is produced in response to stimuli such as bacterial products and pro-inflammatory cytokines. GRO gamma together with its close family members forms a network facilitating the immune cell recruitment and activation at infection sites. Also this chemokine is included in larger chemokine complexes influencing not just immune cells but also modulating angiogenesis and cellular matrix restructuring.

Pathways

GRO gamma significantly contributes to the chemokine signaling pathway and the NF-kB signaling pathway. In these pathways it interacts tightly with proteins like CXCR2 which is essential for the signaling cascade that results in immune cell migration. It also engages with the MAPK pathways impacting cell proliferation and apoptosis. These involvements make GRO gamma vital for orchestrating the inflammation response in humans.

GRO gamma is linked to chronic inflammatory conditions and cancer. Elevated levels of GRO gamma have been observed in rheumatoid arthritis where its interaction with CXCR2 exacerbates inflammation and tissue damage. In cancer particularly in colorectal cancer GRO gamma plays a role in tumor progression by promoting angiogenesis and cell proliferation interacting with VEGF and other cytokines. Understanding its functions and relationships opens potential avenues for therapeutic interventions targeting chronic diseases and cancer.
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Product protocols

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Target data

Ligand for CXCR2 (By similarity). Has chemotactic activity for neutrophils. May play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. In vitro, the processed form GRO-gamma(5-73) shows a fivefold higher chemotactic activity for neutrophilic granulocytes.
See full target information CXCL3
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:4677 PubMed37542037

2023

Targeting of SLC25A22 boosts the immunotherapeutic response in KRAS-mutant colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Qiming Zhou,Yao Peng,Fenfen Ji,Huarong Chen,Wei Kang,Lam-Shing Chan,Hongyan Gou,Yufeng Lin,Pingmei Huang,Danyu Chen,Qinyao Wei,Hao Su,Cong Liang,Xiang Zhang,Jun Yu,Chi Chun Wong

Oncology letters 25:225 PubMed37153045

2023

Study on the mechanism of oral administration of tetrandrine during neoadjuvant chemotherapy for colon cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Li,Junmei Li,Feng Yu,Bo Wang,Bing Liu

British journal of cancer 123:459-470 PubMed32439934

2020

Omental adipocytes promote peritoneal metastasis of gastric cancer through the CXCL2-VEGFA axis.

Applications

Unspecified application

Species

Unspecified reactive species

Makoto Natsume,Takaya Shimura,Hiroyasu Iwasaki,Yusuke Okuda,Kazuki Hayashi,Satoru Takahashi,Hiromi Kataoka
View all publications
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websiteProtocolBooklet
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