Human CXCL5 ELISA Kit
- Recombinant
- SimpleStep
- What is this?
Be the first to review this product! Submit a review
|
(1 Publication)
Human CXCL5 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CXCL5 with a sensitivity of 2 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
View Alternative Names
ENA78, SCYB5, CXCL5, C-X-C motif chemokine 5, ENA-78(1-78), Epithelial-derived neutrophil-activating protein 78, Neutrophil-activating peptide ENA-78, Small-inducible cytokine B5
- sELISA
Supplier Data
Sandwich ELISA - Human CXCL5 ELISA Kit (AB212163)
Interpolated concentrations of native CXCL5 in human serum and plasma samples.
The concentrations of CXCL5 were measured in duplicates, interpolated from the CXCL5 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 100%, plasma (citrate) 50%, plasma (EDTA) 50% and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL5 concentration was determined to be 622.1 pg/mL in serum, 1,977.3 pg/mL in plasma (citrate), 511.4 pg/mL in plasma (EDTA) and 706.2 pg/mL in plasma (heparin).
- sELISA
Supplier Data
Sandwich ELISA - Human CXCL5 ELISA Kit (AB212163)
Interpolated concentrations of native CXCL5 in human PBMC media samples from two different donors.
All samples were diluted 1 : 40 and the concentrations of CXCL5 were measured in duplicates, interpolated from the CXCL5 standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL5 concentration was determined to be 1,188.3 pg/mL in PBMC unstimulated media (Donor 1), 2,702.2 pg/mL in PBMC stimulated media (Donor 1), 567.2 pg/mL in PBMC unstimulated media (Donor 2), and 21,325.6 pg/mL in PBMC stimulated media (Donor 2). PBMC samples were cultured in RPMI media with 10% fetal bovine serum and 1% PenStrep (unstimulated samples). Then PBMC samples were stimulated with 1.5% PHA-M for 48 hours.
- sELISA
Unknown
Sandwich ELISA - Human CXCL5 ELISA Kit (AB212163)
Human CXCL5 standard curve comparison data.
Standard curve comparison between human CSCL5 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 6-fold increase in sensitivity.
- sELISA
Supplier Data
Sandwich ELISA - Human CXCL5 ELISA Kit (AB212163)
Example of human CXCL5 standard curve.
Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Human CXCL5 ELISA Kit (AB212163)
Plasma from three individual healthy human donors (pooled gender) was collected and centrifuged for 15 minutes at 1,000 x g.
Half of the volume of each sample was then additionally centrifuged at 10,000 x g for 10 minutes at 4oC to remove platelets (platelet poor). All plasma samples were diluted 1 : 2 in Sample Diluent NS and measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL5 concentration was determined to be 825 pg/mL in plasma (citrate), 852.0 pg/mL in platelet poor plasma (citrate), 255 pg/mL in plasma (EDTA), 254.2 pg/mL in platelet poor plasma (EDTA), 1,067.8 pg/mL in plasma (heparin), and 1,065.2 pg/mL in platelet poor plasma (heparin).
- sELISA
Supplier Data
Sandwich ELISA - Human CXCL5 ELISA Kit (AB212163)
Serum from ten individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL5 concentration was determined to be 641.9 pg/mL with a range of 14.1 – 1,315.6 pg/mL.
- sELISA
Supplier Data
Sandwich ELISA - Human CXCL5 ELISA Kit (AB212163)
Interpolated concentrations of native CXCL5 in human PBMC stimulated media samples from two different donors.
The concentrations of CXCL5 were measured in duplicates, interpolated from the CXCL5 standard curves and corrected for sample dilution. Undiluted samples are as follows : PBMC media (Donor 1) 10% and PBMC media (Donor 2) 2.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL5 concentration was determined to be 2,702.1 pg/mL in PBMC media (Donor 1), and 21,336.6 pg/mL in PBMC media (Donor 2).
Reactivity data
Product details
Human CXCL5 ELISA Kit (ab212163) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CXCL5 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CXCL5 with 2 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Human CXCL5 is a member of the CXC subfamily of chemokines. Full-length CXCL5 is 114 amino acids (aa) long including a 36 aa signal peptide. This signal peptide is cleaved to produce the 78 aa residue secreted protein (ENA-78). ENA-78 is 52%, 48%, and 51% identical in aa sequence to human GROα, GROβ, and GROγ, respectively. CXCL5 is expressed by fibroblasts and is induced by bacterial lipopolysaccharides. Additionally, this chemokine is a potent chemotaxin involved in neutrophil activation. Furthermore, CXCL5 is produced concomitantly with Interleukin 8 (IL8) in response to stimulation with either Interleukin 1 (IL1) or Tumor Necrosis Factor alpha.
Precision
Recovery
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
CXCL5 affects inflammation and the immune response by modulating neutrophil recruitment to sites of infection or injury. It is a part of the larger network of chemokines that direct leukocyte movement throughout the body. While CXCL5 does not form a complex in a traditional sense it does interact dynamically with CXCR2 receptor to exert its effects. These interactions are pivotal to the protein’s role in orchestrating the immune system’s response to diverse stimuli.
Pathways
CXCL5 plays a part in the chemokine signaling pathway and the inflammatory response pathway. These pathways are important in mediating immune surveillance and inflammation. CXCL5 through these pathways has a strong connection to other CXC family proteins like CXCL1 and the receptor CXCR2 which allows for a coordinated response during immune challenges and recovery processes.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Publications (1)
Recent publications for all applications. Explore the full list and refine your search
Oncology letters 25:225 PubMed37153045
2023
Applications
Unspecified application
Species
Unspecified reactive species
Alternative Products
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com