Human CXCL7 / PBP ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human CXCL7 / PBP in Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.
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- PloS one 17:e02710202022Serum amyloid P component and pro-platelet basic protein in extracellular vesicles or serum are novel markers of liver fibrosis in chronic hepatitis C patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKumiko Shirai,Hayato Hikita,Sadatsugu Sakane,Ryohei Narumi,Jun Adachi,Akira Doi,Satoshi Tanaka,Yuki Tahata,Ryoko Yamada,Takahiro Kodama,Ryotaro Sakamori,Tomohide Tatsumi,Eiji Mita,Takeshi Tomonaga,Tetsuo TakeharaPubMed 35797333
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 4.69 - 300 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 0.51 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
LA-PF4 stimulates DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by human synovial cells. NAP-2 is a ligand for CXCR1 and CXCR2, and NAP-2, NAP-2(73), NAP-2(74), NAP-2(1-66), and most potent NAP-2(1-63) are chemoattractants and activators for neutrophils. TC-1 and TC-2 are antibacterial proteins, in vitro released from activated platelet alpha-granules. CTAP-III(1-81) is more potent than CTAP-III desensitize chemokine-induced neutrophil activation.
Alternative names
CTAP3, CXCL7, SCYB7, TGB1, THBGB1, PPBP, Platelet basic protein, PBP, C-X-C motif chemokine 7, Leukocyte-derived growth factor, Macrophage-derived growth factor, Small-inducible cytokine B7, LDGF, MDGF
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Human CXCL7 / PBP ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human CXCL7 / PBP in Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 4.69 - 300 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 0.51 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Plasma | n 8 | mean - | SD - | C.V. 3.1 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Plasma | n 3 | mean - | SD - | C.V. 4.9 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 90 | Range 84 - 95 % |
Sample type EDTA Plasma | Average % = 94 | Range 89 - 99 % |
Sample type Heparin Plasma | Average % = 88 | Range 84 - 90 % |
Sample type Citrate plasma | Average % = 94 | Range 92 - 96 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human CXCL7 / PBP ELISA Kit (ab216171) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CXCL7 / PBP protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CXCL7 / PBP with 0.51 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
CXCL7 / PBP is a platelet-derived growth factor that belongs to the CXC chemokine family. It is a potent chemoattractant and activator of neutrophils and has been shown to stimulate various cellular processes including DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, and prostaglandin E2 secretion. It also stimulates the formation and secretion of plasminogen activator by synovial cells. Mouse and rat CXCL7 / PBP both have 66% sequence homology compared to human CXCL7 / PBP.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The target CXCL7 also known as PBP PBP-172 or PPBP is a chemokine with a molecular mass of around 11 kDa. It is a platelet basic protein (PBP) belonging to the family of CXC chemokines. This protein is extensively expressed in megakaryocytes and stored in the alpha-granules of platelets. Upon platelet activation CXCL7 is released and becomes a potent attractant and activator of neutrophils. It travels through the bloodstream and impacts extracellular signaling processes.
- Biological function summary
The chemokine plays a significant role in the regulation of inflammation. CXCL7 is not only an individual entity but can form complexes with glycosaminoglycans on cell surfaces enhancing its functionality. This interaction helps in modulating immune responses by recruiting neutrophils and monocytes to sites of injury or infection. The chemokine contributes to wound healing and tissue repair through its involvement in the inflammatory phase.
- Pathways
CXCL7 is involved in the chemokine signaling pathway and is an important mediator in the immune system's response to infections. It is related to other chemokine family proteins like CXCL8 (IL-8) which share similar pathways in inflammatory responses. These proteins interact with specific receptors on leukocytes inducing changes in the cytoskeleton and promoting chemotaxis which is the directed movement towards chemical stimuli.
- Associated diseases and disorders
CXCL7 is associated with various inflammatory conditions and has been implicated in atherosclerosis and rheumatoid arthritis. In these conditions elevated levels of this chemokine correlate with increased recruitment and activation of neutrophils exacerbating inflammation. CXCL7 also shares interactions with other proteins such as CXCL1 affecting the progression and severity of these disorders. The understanding of CXCL7's role in such pathologies offers potential therapeutic avenues for intervention.
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5 product images
Sandwich ELISA - Human CXCL7 / PBP ELISA Kit (ab216171)
Example of human CXCL7 standard curve.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human CXCL7 / PBP ELISA Kit (ab216171)
Interpolated concentrations of native CXCL7 in human serum and plasma samples.
The concentrations of CXCL7 were measured in duplicates, interpolated from the CXCL7 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:4,000, plasma (citrate) 1:8,000, plasma (EDTA) 1:3,000, and plasma (heparin) 1:3,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL7 concentration was determined to be 1,106 ng/mL in neat serum, 1,434 ng/mL in neat plasma (citrate), 636 ng/mL in neat plasma (EDTA), and 604 ng/mL in neat plasma (heparin).
Sandwich ELISA - Human CXCL7 / PBP ELISA Kit (ab216171)
Interpolated concentrations of native CXCL7 in human cell culture supernatant samples.
The concentrations of CXCL7 were measured in duplicates, interpolated from the CXCL7 standard curves and corrected for sample dilution. Undiluted samples are as follows: PBMC supernatant 1:64. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL7 concentration was determined to be 15.61 ng/mL in neat PBMC supernatant.
Sandwich ELISA - Human CXCL7 / PBP ELISA Kit (ab216171)
Interpolated concentrations of native CXCL7 in unstimulated versus stimulated human cell culture supernatant samples.
PBMC media samples were cultured in RPMI media with 10% Fetal Bovine Serum for 48 hours without (unstimulated) or with (stimulated) 1.5% PHA-M.
Sandwich ELISA - Human CXCL7 / PBP ELISA Kit (ab216171)
Serum from ten individual healthy human male donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL7 concentration was determined to be 873 ng/mL with a range of 335 – 2800 ng/mL in neat human serum.
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