Human CXCL7 / PBP ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CXCL7 / PBP with a sensitivity of 0.51 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
View Alternative Names
CTAP3, CXCL7, SCYB7, TGB1, THBGB1, PPBP, Platelet basic protein, PBP, C-X-C motif chemokine 7, Leukocyte-derived growth factor, Macrophage-derived growth factor, Small-inducible cytokine B7, LDGF, MDGF
- sELISA
Supplier Data
Sandwich ELISA - Human CXCL7 / PBP ELISA Kit (AB216171)
Interpolated concentrations of native CXCL7 in unstimulated versus stimulated human cell culture supernatant samples.
PBMC media samples were cultured in RPMI media with 10% Fetal Bovine Serum for 48 hours without (unstimulated) or with (stimulated) 1.5% PHA-M.
- sELISA
Supplier Data
Sandwich ELISA - Human CXCL7 / PBP ELISA Kit (AB216171)
Interpolated concentrations of native CXCL7 in human serum and plasma samples.
The concentrations of CXCL7 were measured in duplicates, interpolated from the CXCL7 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 4,000, plasma (citrate) 1 : 8,000, plasma (EDTA) 1 : 3,000, and plasma (heparin) 1 : 3,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL7 concentration was determined to be 1,106 ng/mL in neat serum, 1,434 ng/mL in neat plasma (citrate), 636 ng/mL in neat plasma (EDTA), and 604 ng/mL in neat plasma (heparin).
- sELISA
Supplier Data
Sandwich ELISA - Human CXCL7 / PBP ELISA Kit (AB216171)
Interpolated concentrations of native CXCL7 in human cell culture supernatant samples.
The concentrations of CXCL7 were measured in duplicates, interpolated from the CXCL7 standard curves and corrected for sample dilution. Undiluted samples are as follows : PBMC supernatant 1 : 64. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL7 concentration was determined to be 15.61 ng/mL in neat PBMC supernatant.
- sELISA
Supplier Data
Sandwich ELISA - Human CXCL7 / PBP ELISA Kit (AB216171)
Serum from ten individual healthy human male donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL7 concentration was determined to be 873 ng/mL with a range of 335 – 2800 ng/mL in neat human serum.
- sELISA
Supplier Data
Sandwich ELISA - Human CXCL7 / PBP ELISA Kit (AB216171)
Example of human CXCL7 standard curve.
Background-subtracted data values (mean +/- SD) are graphed.
Reactivity data
Product details
Human CXCL7 / PBP ELISA Kit (ab216171) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CXCL7 / PBP protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CXCL7 / PBP with 0.51 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
CXCL7 / PBP is a platelet-derived growth factor that belongs to the CXC chemokine family. It is a potent chemoattractant and activator of neutrophils and has been shown to stimulate various cellular processes including DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, and prostaglandin E2 secretion. It also stimulates the formation and secretion of plasminogen activator by synovial cells. Mouse and rat CXCL7 / PBP both have 66% sequence homology compared to human CXCL7 / PBP.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Precision
Recovery
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Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The chemokine plays a significant role in the regulation of inflammation. CXCL7 is not only an individual entity but can form complexes with glycosaminoglycans on cell surfaces enhancing its functionality. This interaction helps in modulating immune responses by recruiting neutrophils and monocytes to sites of injury or infection. The chemokine contributes to wound healing and tissue repair through its involvement in the inflammatory phase.
Pathways
CXCL7 is involved in the chemokine signaling pathway and is an important mediator in the immune system's response to infections. It is related to other chemokine family proteins like CXCL8 (IL-8) which share similar pathways in inflammatory responses. These proteins interact with specific receptors on leukocytes inducing changes in the cytoskeleton and promoting chemotaxis which is the directed movement towards chemical stimuli.
Product protocols
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Target data
Publications (1)
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PloS one 17:e0271020 PubMed35797333
2022
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