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AB100595

Human CXCL9 ELISA Kit

5

(1 Review)

|

(3 Publications)

Human CXCL9 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human CXCL9 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

CMK, MIG, SCYB9, CXCL9, C-X-C motif chemokine 9, Gamma-interferon-induced monokine, Monokine induced by interferon-gamma, Small-inducible cytokine B9, HuMIG

2 Images
Sandwich ELISA - Human CXCL9 ELISA Kit (AB100595)
  • sELISA

Supplier Data

Sandwich ELISA - Human CXCL9 ELISA Kit (AB100595)

Representative Standard Curve using ab100595.

Sandwich ELISA - Human CXCL9 ELISA Kit (AB100595)
  • sELISA

Supplier Data

Sandwich ELISA - Human CXCL9 ELISA Kit (AB100595)

Representative Standard Curve using ab100595.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 20 pg/mL

Range

8.23 - 6000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's CXCL9 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CXCL9 in serum, plasma, and cell culture supernatants.

This assay employs an antibody specific for Human CXCL9 coated on a 96-well plate. Standards and samples are pipetted into the wells and CXCL9 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MIG antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CXCL9 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Optimization may be required with urine samples.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "89 - 108 %", "average": "= 101.3" }, { "sample": "Serum", "range": "82 - 102 %", "average": "= 94.56" }, { "sample": "Plasma", "range": "84 - 103 %", "average": "= 96.32" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100595-1x96Tests", "assetComponentDetails": [ { "size":"2 x 1 Vial", "name":"Recombinant Human CXCL9 Standard (lyophilized)", "number":"AB100595-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human CXCL9", "number":"AB100595-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"CXCL9 Microplate (12 x 8 wells)", "number":"AB100595-CMP07", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100595-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"400X HRP-Streptavidin Concentrate", "number":"AB100595-CMP04", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100595-CMP08", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100595-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100595-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100595-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CXCL9 also known as MIG (monokine induced by gamma interferon) is a small cytokine protein with a molecular weight of approximately 13 kDa. CXCL9 exhibits strong chemotactic properties and is secreted by various cells including endothelial cells macrophages and fibroblasts upon stimulation by interferon-gamma. Its expression primarily occurs in inflamed tissues and is associated with immune responses. Researchers often use tools like a CXCL9 ELISA kit or CXCL9 test to measure its expression levels in biological samples aiding in understanding its role in various conditions.
Biological function summary

This cytokine plays a pivotal role in the immune system by regulating leukocyte trafficking. CXCL9 exerts its function through binding to the CXCR3 receptor attracting T cells towards sites of inflammation or infection. This interaction is significant in mediating immune surveillance and host defense. CXCL9 does not form part of a larger protein complex but its chemokine activity is critical for immune system coordination. Scientific studies often highlight its involvement through CXCL9 function and expression analysis.

Pathways

CXCL9 significantly influences the chemokine signaling pathway and the Th1-type adaptive immune response. Within these pathways it interacts closely with other chemokines like CXCL10 and CXCL11 which also bind to the CXCR3 receptor. These pathways highlight the coordinated mobilization of T cells during immune challenges and inflammation emphasizing how CXCL9 is often examined alongside these related chemokines in research settings.

CXCL9 is notably associated with autoimmune diseases such as rheumatoid arthritis and inflammatory conditions like psoriasis. Its elevated expression is often observed in affected tissues contributing to disease pathogenesis through T cell migration and activation. In these contexts CXCL9 is frequently examined alongside other pro-inflammatory cytokines such as interferon-gamma and TNF-alpha which are known to modulate its expression and activity impacting disease progression and therapeutic strategies.
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Product protocols

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Target data

Cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response. Chemotactic for activated T-cells. Binds to CXCR3.
See full target information CXCL9
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 37:1843-1849 PubMed35810382

2022

CXCL9 Predicts the Risk of Osteoporotic Hip Fracture in a Prospective Cohort of Chinese Men-A Matched Case-Control Study.

Applications

Unspecified application

Species

Unspecified reactive species

Quang Tien Phan,Kevin Yiqiang Chua,Aizhen Jin,Christoph Winkler,Woon-Puay Koh

Scientific reports 10:21360 PubMed33288772

2020

Targeting macrophage priming by polyphyllin VII triggers anti-tumor immunity via STING-governed cytotoxic T-cell infiltration in lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jinglu Yu,Haibin Deng,Zhenye Xu

Microbial pathogenesis 117:148-152 PubMed29432913

2018

Effects of fluconazole on the clinical outcome and immune response in fungal co-infected tuberculosis patients.

Applications

ELISA

Species

Unspecified reactive species

Xiaojuan Ren,Wei Liu,Yi Liu
View all publications
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websiteProtocolBooklet
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