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AB119588

Human CXCL9 ELISA Kit

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(3 Publications)

Human CXCL9 ELISA Kit is a Sandwich ELISA for the measurement of Human CXCL9 in Human in Biofluids, Cell/Tissue Extracts, Cell Culture Media samples.

View Alternative Names

CMK, MIG, SCYB9, CXCL9, C-X-C motif chemokine 9, Gamma-interferon-induced monokine, Monokine induced by interferon-gamma, Small-inducible cytokine B9, HuMIG

1 Images
Sandwich ELISA - Human CXCL9 ELISA Kit (AB119588)
  • sELISA

Supplier Data

Sandwich ELISA - Human CXCL9 ELISA Kit (AB119588)

Representative Standard Curve using ab119588.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 2 pg/mL

Range

31.2 - 2000 pg/mL

Assay Platform

Microplate

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Reactivity data

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Product details

Abcam's Human CXCL9 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human CXCL9 (MIG) in cell culture supernatants, serum and plasma (heparin, EDTA).

A CXCL9 specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for CXCL9 is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human CXCL9 amount of sample captured in plate.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CXCL9 also known as MIG (monokine induced by gamma interferon) is a small cytokine protein with a molecular weight of approximately 13 kDa. CXCL9 exhibits strong chemotactic properties and is secreted by various cells including endothelial cells macrophages and fibroblasts upon stimulation by interferon-gamma. Its expression primarily occurs in inflamed tissues and is associated with immune responses. Researchers often use tools like a CXCL9 ELISA kit or CXCL9 test to measure its expression levels in biological samples aiding in understanding its role in various conditions.
Biological function summary

This cytokine plays a pivotal role in the immune system by regulating leukocyte trafficking. CXCL9 exerts its function through binding to the CXCR3 receptor attracting T cells towards sites of inflammation or infection. This interaction is significant in mediating immune surveillance and host defense. CXCL9 does not form part of a larger protein complex but its chemokine activity is critical for immune system coordination. Scientific studies often highlight its involvement through CXCL9 function and expression analysis.

Pathways

CXCL9 significantly influences the chemokine signaling pathway and the Th1-type adaptive immune response. Within these pathways it interacts closely with other chemokines like CXCL10 and CXCL11 which also bind to the CXCR3 receptor. These pathways highlight the coordinated mobilization of T cells during immune challenges and inflammation emphasizing how CXCL9 is often examined alongside these related chemokines in research settings.

CXCL9 is notably associated with autoimmune diseases such as rheumatoid arthritis and inflammatory conditions like psoriasis. Its elevated expression is often observed in affected tissues contributing to disease pathogenesis through T cell migration and activation. In these contexts CXCL9 is frequently examined alongside other pro-inflammatory cytokines such as interferon-gamma and TNF-alpha which are known to modulate its expression and activity impacting disease progression and therapeutic strategies.
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Product protocols

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Target data

Cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response. Chemotactic for activated T-cells. Binds to CXCR3.
See full target information CXCL9
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Human molecular genetics 33:872-883 PubMed38340007

2024

Muscle eosinophilia is a hallmark of chronic disease in facioscapulohumeral muscular dystrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Andreia M Nunes,Monique M Ramirez,Enrique Garcia-Collazo,Takako Iida Jones,Peter L Jones

Experimental and therapeutic medicine 18:4845-4851 PubMed31772648

2019

CXCL9 regulates acetaminophen-induced liver injury via CXCR3.

Applications

Unspecified application

Species

Unspecified reactive species

Xi Song,Yuying Shen,Yiqun Lao,Zhen Tao,Juan Zeng,Jihui Wang,Huiling Wu

North American journal of medical sciences 6:84-8 PubMed24695523

2014

Chemokines Responses to Ascaris Lumbricoides Sole Infection and Co-infection with Hookworm among Nigerians.

Applications

sELISA

Species

Human

Omorodion Oriri Asemota,O P G Nmorsi,C Isaac,E M Odoya,J Akinseye,O Isaac
View all publications
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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