Name: Human CXCL9 ELISA Kit
SKU: ab219047
Rating: 5 (1 reviews)

Human CXCL9 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CXCL9 with a sensitivity of 5 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

Images

ELISA - Human CXCL9 ELISA Kit (AB219047), expandable thumbnail

Publications

Key facts

Detection method: Colorimetric
Sample types: Cell culture extracts, Tissue Extracts, Cell culture supernatant
Assay type: Sandwich (quantitative)
Reactive species: Human
Range: 28.13 - 1800 pg/mL
Assay time: 1h 30m
Sensitivity: = 5 pg/mL

Reactivity data

Application	Reactivity	Dilution info	Notes
Application sELISA	Reactivity Reacts	Dilution info -	Notes -

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Target data

See full target information CXCL9

Function

Cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response. Chemotactic for activated T-cells. Binds to CXCR3.

Alternative names

CMK, MIG, SCYB9, CXCL9, C-X-C motif chemokine 9, Gamma-interferon-induced monokine, Monokine induced by interferon-gamma, Small-inducible cytokine B9, HuMIG

What's included?

1 x 96 Tests

Components

10X Human CXCL9 Capture Antibody

1 x 600 µL

10X Human CXCL9 Detector Antibody

1 x 600 µL

10X Wash Buffer PT (ab206977)

1 x 20 mL

50X Cell Extraction Enhancer Solution (ab193971)

1 x 1 mL

5X Cell Extraction Buffer PTR (ab193970)

1 x 10 mL

Antibody Diluent 4BI

1 x 6 mL

Human CXCL9 Lyophilized Recombinant Protein

2 x 1 Vial

Plate Seals

1 x 1 Unit

Sample Diluent NS (ab193972)

1 x 50 mL

SimpleStep Pre-Coated 96-Well Microplate (ab206978)

1 x 1 Unit

Stop Solution

1 x 12 mL

TMB Development Solution

1 x 12 mL

Key facts

Detection method: Colorimetric
Sample types: Cell culture extracts, Tissue Extracts, Cell culture supernatant
Assay type: Sandwich (quantitative)
Reactive species: Human
Range: 28.13 - 1800 pg/mL
Assay time: 1h 30m
Assay Platform: Pre-coated microplate (12 x 8 well strips)
Sensitivity: = 5 pg/mL

Precision

Intra assay

Sample	n	C.V.
Sample Overall	n 3	C.V. 3.4

Inter assay

Sample	n	C.V.
Sample Overall	n 5	C.V. 5.9

Recovery

Sample specific recovery

Sample type	Average %	Range
Sample type Cell culture supernatant	Average % = 90	Range 86 - 92 %
Sample type Tissue Extracts	Average % = 109	Range 104 - 116 %

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Storage information: +4°C

Notes

Human CXCL9 ELISA Kit ab219047 is a rapid single-wash 90-min Sandwich ELISA to measure Human CXCL9 in cell culture extracts, cell culture supernatant, tissue extracts. This SimpleStep sensitivity is 5 pg/mL.

How the assay works

Human CXCL9 SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human CXCL9 ELISA Kit ab219047 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-CXCL9 antibody [EPR18732-17] - BSA and Azide free (Capture) Anti-CXCL9 antibody [EPR18732-24] - BSA and Azide free (Detector) ab243004
- Anti-CXCL9 antibody [EPR18732-24] - BSA and Azide free (Detector) Anti-CXCL9 antibody [EPR18732-24] - BSA and Azide free (Detector) ab243004

CXCL9, is a small cytokine belonging to the CXC chemokine subfamily that lacks an ELR motif in front of the first cysteine. CXCL9, also known as MIG, (Monokine Induced by Gamma Interferon) is a T-cell chemoattractant, which is induced by Interferon Gamma. This subfamily also includes Interferon Gamma Induced Protein 10 (IP-10 or CXCL10) and Interferon Inducible T-cell Alpha Chemoattractant (I-TAC or CXCL11) whose genes are located near the gene for MIG on human chromosome 4. MIG, IP-10 and I-TAC all elicit their chemotactic functions by interacting with the G protein coupled chemokine receptor CXCR3 (GPR9 or CD183).

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CXCL9 also known as MIG (monokine induced by gamma interferon) is a small cytokine protein with a molecular weight of approximately 13 kDa. CXCL9 exhibits strong chemotactic properties and is secreted by various cells including endothelial cells macrophages and fibroblasts upon stimulation by interferon-gamma. Its expression primarily occurs in inflamed tissues and is associated with immune responses. Researchers often use tools like a CXCL9 ELISA kit or CXCL9 test to measure its expression levels in biological samples aiding in understanding its role in various conditions.

Biological function summary

This cytokine plays a pivotal role in the immune system by regulating leukocyte trafficking. CXCL9 exerts its function through binding to the CXCR3 receptor attracting T cells towards sites of inflammation or infection. This interaction is significant in mediating immune surveillance and host defense. CXCL9 does not form part of a larger protein complex but its chemokine activity is critical for immune system coordination. Scientific studies often highlight its involvement through CXCL9 function and expression analysis.

Pathways

CXCL9 significantly influences the chemokine signaling pathway and the Th1-type adaptive immune response. Within these pathways it interacts closely with other chemokines like CXCL10 and CXCL11 which also bind to the CXCR3 receptor. These pathways highlight the coordinated mobilization of T cells during immune challenges and inflammation emphasizing how CXCL9 is often examined alongside these related chemokines in research settings.

Associated diseases and disorders

CXCL9 is notably associated with autoimmune diseases such as rheumatoid arthritis and inflammatory conditions like psoriasis. Its elevated expression is often observed in affected tissues contributing to disease pathogenesis through T cell migration and activation. In these contexts CXCL9 is frequently examined alongside other pro-inflammatory cytokines such as interferon-gamma and TNF-alpha which are known to modulate its expression and activity impacting disease progression and therapeutic strategies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

ELISA - Human CXCL9 ELISA Kit (ab219047)
Sandwich ELISA - Human CXCL9 ELISA Kit (ab219047)
Example of human CXCL9 standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human CXCL9 ELISA Kit (ab219047)
Example of human CXCL9 standard curve in 1X Cell Extraction Buffer PTR.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human CXCL9 ELISA Kit (ab219047)
Human CXCL9 standard curve comparison data.
Standard curve comparison between human CXCL9 SimpleStep ELISA^® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.
Sandwich ELISA - Human CXCL9 ELISA Kit (ab219047)
Interpolated concentrations of native CXCL9 in PHA-M stimulated and unstimulated human PBMC cell culture supernatant (2 days) samples.
The concentrations of CXCL9 were measured in duplicates, interpolated from the MIG standard curves and corrected for sample dilution. Undiluted samples are as follows: PHA-M stimulated PBMC supernatant 2.5% and unstimulated PBMC supernatant 100% (neat). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL9 concentration was determined to be 20.4 ng/mL in neat PHA-M stimulated PBMC supernatant and 1.62 ng/mL in neat unstimulated PBMC supernatant.
Sandwich ELISA - Human CXCL9 ELISA Kit (ab219047)
Interpolated concentrations of native CXCL9 in PHA-M stimulated human PBMC cell extract based on a 200 μg/mL extract load and human thyroid tissue extract based on a 500 μg/mL extract load.
The concentrations of CXCL9 were measured in duplicate and interpolated from the MIG standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL9 concentration was determined to be 993.9 pg/mL in PHA-M stimulated PBMC cell extract and 824.9 pg/mL in thyroid tissue extract.
Sandwich ELISA - Human CXCL9 ELISA Kit (ab219047)
Comparison of CXCL9 in unstimulated and PHA-M stimulated human PBMC cell supernatants.
Human PBMC cells were cultured in the absence or presence of 1.5% PHA-M for 2 days. The concentrations of CXCL9 were measured in three different dilutions of the supernatant samples in duplicates and interpolated from the MIG standard curve. The interpolated values are plotted (mean +/- SD, n=2). The mean MIG concentration was determined to be 20.4 pg/mL in PHA-M stimulated PBMC cell supernatant, 1.6 pg/mL in unstimulated supernatants and undetectable in media (not shown).