Human CXCL9 ELISA Kit, Fluorescent is a single-wash 90-min Simplestep used to quantify Human CXCL9 with a sensitivity of 2.6 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Fluorescent Sandwich ELISA - 530/570/590 nm readout : works on any standard plate reader
Images
Key facts
- Detection method
- Fluorescent
- Sample types
- Tissue Extracts, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 2.93 - 3000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 2.6 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response. Chemotactic for activated T-cells. Binds to CXCR3.
Alternative names
CMK, MIG, SCYB9, CXCL9, C-X-C motif chemokine 9, Gamma-interferon-induced monokine, Monokine induced by interferon-gamma, Small-inducible cytokine B9, HuMIG
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Human CXCL9 ELISA Kit, Fluorescent is a single-wash 90-min Simplestep used to quantify Human CXCL9 with a sensitivity of 2.6 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Fluorescent Sandwich ELISA - 530/570/590 nm readout : works on any standard plate reader
Key facts
- Detection method
- Fluorescent
- Sample types
- Tissue Extracts, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 2.93 - 3000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 2.6 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Supernatant | n 3 | C.V. 3.4 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Supernatant | n 5 | C.V. 4.9 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 90 | Range 86 - 92 % |
Sample type Tissue Extracts | Average % = 109 | Range 104 - 116 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
CXCL9 in vitro CatchPoint® SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of CXCL9 protein in human plasma cell culture supernatants, and tissue extract samples.
This CatchPoint SimpleStep ELISA kit has been optimized for Molecular Devices Microplate Readers. Click here for a list of recommended Microplate Readers.
If using a Molecular Devices' plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint SimpleStep ELISA Kits is available with all the protocol and analysis settings at www.softmaxpro.org
The CatchPoint SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPoint HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission.
CXCL9 is a small cytokine belonging to the CXC chemokine subfamily that lacks an ELR motif in front of the first cysteine. CXCL9, also known as MIG (Monokine Induced by Gamma Interferon) is a T-cell chemoattractant, which is induced by Interferon Gamma, This subfamily also includes Interferon Gamma Induced Protein 10 (IP-10 or CXCL10) and Interferon Inducible T-cell Alpha Chemoattractant (I-TAC or CXCL11) whose genes are located near the gene for CXCL9 on human chromosome 4, CXCL9, IP-10, and I-TAC all elicit their chemotactic functions by interacting with the G protein coupled chemokine receptor CXCR3 (GPR9 or CD183).
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CXCL9 also known as MIG (monokine induced by gamma interferon) is a small cytokine protein with a molecular weight of approximately 13 kDa. CXCL9 exhibits strong chemotactic properties and is secreted by various cells including endothelial cells macrophages and fibroblasts upon stimulation by interferon-gamma. Its expression primarily occurs in inflamed tissues and is associated with immune responses. Researchers often use tools like a CXCL9 ELISA kit or CXCL9 test to measure its expression levels in biological samples aiding in understanding its role in various conditions.
- Biological function summary
This cytokine plays a pivotal role in the immune system by regulating leukocyte trafficking. CXCL9 exerts its function through binding to the CXCR3 receptor attracting T cells towards sites of inflammation or infection. This interaction is significant in mediating immune surveillance and host defense. CXCL9 does not form part of a larger protein complex but its chemokine activity is critical for immune system coordination. Scientific studies often highlight its involvement through CXCL9 function and expression analysis.
- Pathways
CXCL9 significantly influences the chemokine signaling pathway and the Th1-type adaptive immune response. Within these pathways it interacts closely with other chemokines like CXCL10 and CXCL11 which also bind to the CXCR3 receptor. These pathways highlight the coordinated mobilization of T cells during immune challenges and inflammation emphasizing how CXCL9 is often examined alongside these related chemokines in research settings.
- Associated diseases and disorders
CXCL9 is notably associated with autoimmune diseases such as rheumatoid arthritis and inflammatory conditions like psoriasis. Its elevated expression is often observed in affected tissues contributing to disease pathogenesis through T cell migration and activation. In these contexts CXCL9 is frequently examined alongside other pro-inflammatory cytokines such as interferon-gamma and TNF-alpha which are known to modulate its expression and activity impacting disease progression and therapeutic strategies.
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4 product images
Sandwich ELISA - Human CXCL9 ELISA Kit, Fluorescent (ab270891)
Example of human CXCL9 standard curve in Sample Diluent NS.
The CXCL9 standard curve was prepared as described in Section 10. Raw data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human CXCL9 ELISA Kit, Fluorescent (ab270891)
Interpolated concentrations of native CXCL9 in PHA-M stimulated and unstimulated human PBMC cell culture supernatant (2 days) samples.
The concentrations of CXCL9 were measured in duplicates, interpolated from the CXCL9 standard curves and corrected for sample dilution. Undiluted samples are as follows: PHA-M stimulated PBMC supernatant 2.5% and unstimulated PBMC supernatant 100% (neat). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL9 concentration was determined to be 20.4 ng/mL in neat PHA-M stimulated PBMC supernatant and 1.62 ng/mL in neat unstimulated PBMC supernatant.
Sandwich ELISA - Human CXCL9 ELISA Kit, Fluorescent (ab270891)
Interpolated concentrations of native CXCL9 in PHA-M stimulated human PBMC cell extract based on a 200 μg/mL extract load.
The concentrations of CXCL9 were measured in duplicate and interpolated from the CXCL9 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL9 concentration was determined to be 993.9 pg/mL in PHA-M stimulated PBMC cell extract and 824.9 pg/mL in thyroid tissue extract.
Sandwich ELISA - Human CXCL9 ELISA Kit, Fluorescent (ab270891)
Comparison of CXCL9 in unstimulated and PHA-M stimulated human PBMC cell supernatants.
Human PBMC cells were cultured in the absence or presence of 1.5% PHA-M for 2 days. The concentrations of CXCL9 were measured in three different dilutions of the supernatant samples in duplicates and interpolated from the CXCL9 standard curve. The interpolated values are plotted (mean +/- SD, n=3). The mean CXCL9 concentration was determined to be 21.3 ng/mL in PHA-M stimulated PBMC cell supernatant, 1.6 ng/mL in unstimulated supernatants and undetectable in media (not shown).
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