Human Cyclin D1 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Cyclin D1 in Cell culture extracts, Tissue Extracts samples.
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Publications
- BioMed research international 2020:93965122020Luteolin Suppresses the Proliferation of Gastric Cancer Cells and Acts in Synergy with Oxaliplatin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi-Qun Ren et. al.PubMed 32149146
- Molecular biology reports 46:657-6672018Expression of long non-coding RNA CCHE1 in colorectal carcinoma: correlations with clinicopathological features and ERK/COX-2 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHanaa H Gaballah,Rasha A Gaber,Mohamed A Elrashidy,Dina A Elshahat,Mohamed A Hablus,Abla M EbeidPubMed 30484108
Key facts
- Detection method
Colorimetric
- Sample types
Cell culture extracts, Tissue Extracts
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
0.187 - 12 ng/mL
- Assay time
1h 30m
- Sensitivity
= 33 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner.
Alternative names
G1/S-specific cyclin-D1, B-cell lymphoma 1 protein, BCL-1 oncogene, PRAD1 oncogene, BCL-1, PRAD1, BCL1, CCND1
What's included?
Recommended products
Human Cyclin D1 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Cyclin D1 in Cell culture extracts, Tissue Extracts samples.
Alternative names
G1/S-specific cyclin-D1, B-cell lymphoma 1 protein, BCL-1 oncogene, PRAD1 oncogene, BCL-1, PRAD1, BCL1, CCND1
Key facts
- Detection method
Colorimetric
- Sample types
Cell culture extracts, Tissue Extracts
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
0.187 - 12 ng/mL
- Assay time
1h 30m
- Assay Platform
Pre-coated microplate (12 x 8 well strips)
- Sensitivity
= 33 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 5 | mean - | SD - | C.V. 5 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 3 | mean - | SD - | C.V. 7 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture extracts | Average % = 122 | Range 117 - 124 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Human Cyclin D1 ELISA Kit (ab214571) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Cyclin D1 protein in cell culture extracts and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Cyclin D1 with 33 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Activity of cyclin dependent kinases CDK4 and CDK6 is regulated by the abundance of their cyclin D partners, by phosphorylation, and by association with CDK inhibitors including Kip proteins. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. The inactive ternary complex of cyclin D1/CDK4 and p27 Kip1 requires extracellular mitogenic stimuli for the dissociation and degradation of p27 concomitant with a rise in cyclin D1 levels to allow G1/S progression. Active cyclin D1-CDK4 complex phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1. The phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Upon withdrawal of growth factors, levels of cyclin D1 protein are reduced via downregulation of protein expression and phosphorylation-dependent degradation.
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It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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5 product images
Sandwich ELISA - Human Cyclin D1 ELISA Kit (ab214571)
Example of human Cyclin D1 standard curve.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Cyclin D1 ELISA Kit (ab214571)
Interpolated concentrations of native Cyclin D1 in human MCF-7 cell extracts based on a 250 µg/mL extract load.
MCF-7 cells were grown in media containing 10% FBS (untreated), serum starved for last 24 hours (serum starved), or serum starved for last 24 hours and then treated with 10% FBS for 6 hours (serum treated). The concentrations of Cyclin D1 were measured in duplicate and interpolated from the Cyclin D1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cyclin D1 concentration was determined to be 8.3 ng/mL in untreated MCF-7 cell extract, 3.9 ng/mL in serum starved MCF-7 cell extract, and 11.5 ng/mL in serum treated MCF-7 cell extract.
Sandwich ELISA - Human Cyclin D1 ELISA Kit (ab214571)
Interpolated concentrations of native Cyclin D1 in human cell lines.
Interpolated concentrations of native Cyclin D1 in human MDA-MB-231 cell extract based on a 100 μg/mL extract load, SHSY-5Y cell extract based on a 500 μg/mL extract load, and HepG2 cell extract based on a 250 μg/mL extract load. The concentrations of Cyclin D1 were measured in duplicate and interpolated from the Cyclin D1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cyclin D1 concentration was determined to be 0.24 ng/mL in MDA-MB-231 cell extract, 2.89 ng/mL in SHSY-5Y cell extract, and 11.7 ng/mL in HepG2 cell extract.
Sandwich ELISA - Human Cyclin D1 ELISA Kit (ab214571)
Comparison of Cyclin D1 concentrations in untreated MCF-7, serum starved MCF-7, and serum rescued MCF-7 cell extracts.
MCF-7 cells were grown in media containing 10% FBS (untreated), serum starved for last 24 hours (serum starved), or serum starved for last 24 hours and then treated with 10% FBS for 6 hours (serum treated). The concentrations of Cyclin D1 were measured in three 2-fold serial dilutions starting at 250 μg/mL in duplicates, interpolated from the Cyclin D1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted in ng of Cyclin D1 per mg of total extracted protein (mean +/- SD, n=3). The mean Cyclin D1 concentration was determined to be 35.8 ng/mg in untreated MCF-7 cell extract, 15.7 ng/mg in serum starved MCF-7 cell extract, and 48.2 ng/mg in serum treated MCF-7 cell extract.
Sandwich ELISA - Human Cyclin D1 ELISA Kit (ab214571)
Interpolated concentrations of native Cyclin D1 in mouse NIH/3T3, mouse C2C12 and rat H9C2 cell extracts.
The concentrations of Cyclin D1 were measured in three 2-fold serial dilutions starting at 500 μg/mL for NIH/3T3 and C2C12 cell extracts and 250 μg/mL for H9C2 cell extracts in duplicates, interpolated from the human Cyclin D1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted in ng of cyclin D1 per mg of total extracted protein (mean +/- SD, n=2-3). The mean Cyclin D1 concentration was determined to be 1.7 ng/mg in NIH/3T3 cell extract, 23.1 ng/mg in C2C12 cell extract, and 43.1 ng/mg in H9C2 cell extract.
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