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AB226894

Human Cyclin D2 ELISA kit

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Human Cyclin D2 ELISA kit is a single-wash 90-min Simplestep used to quantify Human Cyclin D2 with a sensitivity of 23 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
3 Images
Sandwich ELISA - Human Cyclin D2 ELISA kit (AB226894)
  • sELISA

Supplier Data

Sandwich ELISA - Human Cyclin D2 ELISA kit (AB226894)

Representative image of Human Cyclin D2 ELISA kit (ab226894)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Human Cyclin D2 ELISA kit Capture Antibody

- 10X Human Cyclin D2 Detector Antibody

- 10X Human Cyclin D2 ELISA kit Lyophilised Purified Protein (2 vials)

- Human Cyclin D2 ELISA kit 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody Diluent 4BI

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

Sandwich ELISA - Human Cyclin D2 ELISA kit (AB226894)
  • sELISA

Supplier Data

Sandwich ELISA - Human Cyclin D2 ELISA kit (AB226894)

Example of Human Cyclin D2 standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Cyclin D2 ELISA kit (AB226894)
  • sELISA

Supplier Data

Sandwich ELISA - Human Cyclin D2 ELISA kit (AB226894)

Interpolated concentrations of native Cyclin D2 in Human HEK-293T and HL-60 cell extract samples.

Interpolated concentrations of native Cyclin D2 in Human HEK-293T cell extract samples based on a 1,250 μg/ml extract load, and interpolated concentrations of spiked Cyclin D2 into HL-60 Cell Extract samples at an extract load of 500 μg/ml. The concentrations of Cyclin D2 were measured in duplicates, interpolated from the Cyclin D2 standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cyclin D2 concentration was determined to be 2,612 pg/mL in HEK-293T Cell Extract.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 23 pg/mL

Range

50 - 3200 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB207604

Anti-Cyclin D2 antibody [EPR19659]

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-Cyclin D2 antibody [EPR19659] (AB207604)

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Proteins & Peptides

AB116405

Recombinant Human Cyclin D2 protein (denatured) (His tag N-Terminus)

SDS-PAGE - Recombinant Human Cyclin D2 protein (denatured) (His tag N-Terminus) (AB116405)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Cyclin D2 ELISA kit (ab226894) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Cyclin D2 protein in cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Cyclin D2 with 23 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

G1/S-Specific cyclin D2, or Cyclin D2, belongs to the conserved cyclin family of proteins, which have a periodicity in expression levels during the cell cycle and is encoded by the CCND2 gene. Cyclin D2 is a regulatory component of the cyclin D2-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family, including RB1 and regulates the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes, which are responsible for the progression through the G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated into the nucleus through interaction with KIP/CIP family members.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "HEK-293T Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.9" }, { "reproducibilityType": "Intra", "sample": "HEK-293T Extract", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.7" } ]
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Recovery

[ { "sample": "Cell culture extracts", "range": "86 - 97 %", "average": "= 92" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab226894-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB226894-CMP11", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB226894-CMP07", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB226894-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB226894-CMP02", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB226894-CMP12", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB226894-CMP03", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB226894-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB226894-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB226894-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Cyclin D2 Lyophilized Recombinant Protein", "number":"AB226894-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Cyclin D2 Capture Antibody", "number":"AB226894-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Cyclin D2 Detector Antibody", "number":"AB226894-CMP09", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Regulatory component of the cyclin D2-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition (PubMed : 18827403, PubMed : 8114739). Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase (PubMed : 18827403, PubMed : 8114739). Hypophosphorylates RB1 in early G(1) phase (PubMed : 18827403, PubMed : 8114739). Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals (PubMed : 18827403, PubMed : 8114739).
See full target information CCND2

Alternative Names

G1/S-specific cyclin-D2, CCND2
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Product promise

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