Human Cyclin D2 ELISA kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Cyclin D2 in Cell culture extracts samples.
Images
Key facts
- Detection method
Colorimetric
- Sample types
Cell culture extracts
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
50 - 3200 pg/mL
- Assay time
1h 30m
- Sensitivity
= 23 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Regulatory component of the cyclin D2-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D2/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex (By similarity).
Alternative names
G1/S-specific cyclin-D2, CCND2
What's included?
Recommended products
Human Cyclin D2 ELISA kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Cyclin D2 in Cell culture extracts samples.
Alternative names
G1/S-specific cyclin-D2, CCND2
Key facts
- Detection method
Colorimetric
- Sample types
Cell culture extracts
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
50 - 3200 pg/mL
- Assay time
1h 30m
- Assay Platform
Pre-coated microplate (12 x 8 well strips)
- Sensitivity
= 23 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample HEK-293T Extract | n 5 | mean - | SD - | C.V. 2.7 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample HEK-293T Extract | n 3 | mean - | SD - | C.V. 6.9 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture extracts | Average % = 92 | Range 86 - 97 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Human Cyclin D2 ELISA kit (ab226894) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Cyclin D2 protein in cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Cyclin D2 with 23 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
G1/S-Specific cyclin D2, or Cyclin D2, belongs to the conserved cyclin family of proteins, which have a periodicity in expression levels during the cell cycle and is encoded by the CCND2 gene. Cyclin D2 is a regulatory component of the cyclin D2-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family, including RB1 and regulates the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes, which are responsible for the progression through the G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated into the nucleus through interaction with KIP/CIP family members.
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2 product images
Sandwich ELISA - Human Cyclin D2 ELISA kit (ab226894)
Example of Human Cyclin D2 standard curve in 1X Cell Extraction Buffer PTR.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Cyclin D2 ELISA kit (ab226894)
Interpolated concentrations of native Cyclin D2 in Human HEK-293T and HL-60 cell extract samples.
Interpolated concentrations of native Cyclin D2 in Human HEK-293T cell extract samples based on a 1,250 μg/ml extract load, and interpolated concentrations of spiked Cyclin D2 into HL-60 Cell Extract samples at an extract load of 500 μg/ml. The concentrations of Cyclin D2 were measured in duplicates, interpolated from the Cyclin D2 standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cyclin D2 concentration was determined to be 2,612 pg/mL in HEK-293T Cell Extract.
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