JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB231929

Human Cyclin E1 ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Human Cyclin E1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Cyclin E1 with a sensitivity of 1.2 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CCNE, CCNE1, G1/S-specific cyclin-E1

4 Images
Sandwich ELISA - Human Cyclin E1 ELISA Kit (AB231929)
  • sELISA

Supplier Data

Sandwich ELISA - Human Cyclin E1 ELISA Kit (AB231929)

Representative image of Human Cyclin E1 ELISA Kit (ab231929)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Human Cyclin E1 ELISA Kit Capture Antibody

- 10X Human Cyclin E1 Detector Antibody

- 10X Human Cyclin E1 ELISA Kit Lyophilised Purified Protein (2 vials)

- Human Cyclin E1 ELISA Kit 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody Diluent 4BI

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

Sandwich ELISA - Human Cyclin E1 ELISA Kit (AB231929)
  • sELISA

Unknown

Sandwich ELISA - Human Cyclin E1 ELISA Kit (AB231929)

Interpolated concentrations of native Cyclin E1 in HeLa cell extract based on a 1,500 μg/mL extract load.

The concentrations of Cyclin E1 were measured in duplicate and interpolated from the Cyclin E1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cyclin E1 concentration was determined to be 54 ng/mL in HeLa cell extract.

Sandwich ELISA - Human Cyclin E1 ELISA Kit (AB231929)
  • sELISA

Unknown

Sandwich ELISA - Human Cyclin E1 ELISA Kit (AB231929)

Interpolated concentrations of native Cyclin E1 in JAR cell extract based on a 1,000 μg/mL extract load.

The concentrations of Cyclin E1 were measured in duplicate and interpolated from the Cyclin E1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cyclin E1 concentration was determined to be 163 ng/mL in JAR cell extract.

Sandwich ELISA - Human Cyclin E1 ELISA Kit (AB231929)
  • sELISA

Supplier Data

Sandwich ELISA - Human Cyclin E1 ELISA Kit (AB231929)

Example of human Cyclin E1 standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 1.2 ng/mL

Range

3.91 - 250 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human Cyclin E1 ELISA Kit (ab231929) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Cyclin E1 protein in cell culture extracts and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Cyclin E1 with 1.2 ng/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

The standard is affinity purified.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Cell Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "12.4" }, { "reproducibilityType": "Intra", "sample": "Cell Extract", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.1" } ]
style
left-column

Recovery

[ { "sample": "Cell culture extracts", "range": "98 - 108 %", "average": "= 104" }, { "sample": "Tissue Extracts", "range": "98 - 102 %", "average": "= 101" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab231929-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB231929-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB231929-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB231929-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB231929-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB231929-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB231929-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Cyclin E1 Capture Antibody", "number":"AB231929-CMP08", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB231929-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Cyclin E1 Lyophilized Recombinant Protein", "number":"AB231929-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Cyclin E1 Detector Antibody", "number":"AB231929-CMP09", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB231929-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB231929-CMP02", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column
style
left-column

Product protocols

style
left-column

Target data

Essential for the control of the cell cycle at the G1/S (start) transition.
See full target information CCNE1
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Assay Kits

AB214571

Human Cyclin D1 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Cyclin D1 ELISA Kit (AB214571)

  • 0
  • 0
Assay Kits

AB289900

Human CDK6 ELISA Kit

SimpleStep

Sandwich ELISA - Human CDK6 ELISA Kit (AB289900)

  • 0
  • 0
Primary Antibodies

AB33911

Anti-Cyclin E1 antibody [EP435E]

BOND RX™ Validated|KO Validated|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Cyclin E1 antibody [EP435E] (AB33911)

  • 4
  • 8
Assay Kits

AB171571

Human p53 ELISA Kit

SimpleStep

ELISA - Human p53 ELISA Kit (AB171571)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com