Human Cyclin E1 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Cyclin E1 in Cell culture extracts, Tissue Extracts samples.
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Key facts
- Detection method
Colorimetric
- Sample types
Cell culture extracts, Tissue Extracts
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
3.91 - 250 ng/mL
- Assay time
1h 30m
- Sensitivity
= 1.2 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Essential for the control of the cell cycle at the G1/S (start) transition.
Alternative names
G1/S-specific cyclin-E1, CCNE, CCNE1
What's included?
Recommended products
Human Cyclin E1 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Cyclin E1 in Cell culture extracts, Tissue Extracts samples.
Alternative names
G1/S-specific cyclin-E1, CCNE, CCNE1
Key facts
- Detection method
Colorimetric
- Sample types
Cell culture extracts, Tissue Extracts
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
3.91 - 250 ng/mL
- Assay time
1h 30m
- Assay Platform
Pre-coated microplate (12 x 8 well strips)
- Sensitivity
= 1.2 ng/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Cell Extract | n 5 | mean - | SD - | C.V. 2.1 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Cell Extract | n 3 | mean - | SD - | C.V. 12.4 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture extracts | Average % = 104 | Range 98 - 108 % |
Sample type Tissue Extracts | Average % = 101 | Range 98 - 102 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Human Cyclin E1 ELISA Kit (ab231929) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Cyclin E1 protein in cell culture extracts and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Cyclin E1 with 1.2 ng/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
The standard is affinity purified.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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3 product images
Sandwich ELISA - Human Cyclin E1 ELISA Kit (ab231929)
Example of human Cyclin E1 standard curve in 1X Cell Extraction Buffer PTR.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Cyclin E1 ELISA Kit (ab231929)
Interpolated concentrations of native Cyclin E1 in JAR cell extract based on a 1,000 μg/mL extract load.
The concentrations of Cyclin E1 were measured in duplicate and interpolated from the Cyclin E1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cyclin E1 concentration was determined to be 163 ng/mL in JAR cell extract.
Sandwich ELISA - Human Cyclin E1 ELISA Kit (ab231929)
Interpolated concentrations of native Cyclin E1 in HeLa cell extract based on a 1,500 μg/mL extract load.
The concentrations of Cyclin E1 were measured in duplicate and interpolated from the Cyclin E1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cyclin E1 concentration was determined to be 54 ng/mL in HeLa cell extract.
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