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AB277409

Human Cyclophilin B ELISA Kit

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Human Cyclophilin B ELISA Kit is a Sandwich ELISA for the measurement of Human Cyclophilin B in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

CYPB, PPIB, Peptidyl-prolyl cis-trans isomerase B, PPIase B, CYP-S1, Cyclophilin B, Rotamase B, S-cyclophilin, SCYLP

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ELISA - Human Cyclophilin B ELISA Kit (AB277409)
  • ELISA

Supplier Data

ELISA - Human Cyclophilin B ELISA Kit (AB277409)

These standard curves are for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.45 ng/mL

Range

0.41 - 100 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB16045

Anti-Cyclophilin B antibody - Loading Control

KO Validated|Lab Essentials

Western blot - Anti-Cyclophilin B antibody - Loading Control (AB16045)

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Proteins & Peptides

AB53870

Recombinant Cyclophilin B protein (Tag Free)

Bioactive

SDS-PAGE - Recombinant Cyclophilin B protein (Tag Free) (AB53870)

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  • 0
Cell Lines & Lysates

AB257037

Human PPIB (Cyclophilin B) knockout HeLa cell lysate

Western blot - Human PPIB (Cyclophilin B) knockout HeLa cell lysate (AB257037)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Cyclophilin B ELISA Kit (ab277409) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Cyclophilin B in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Human Cyclophilin B coated on a 96-well plate. Standards and samples are pipetted into the wells and Human Cyclophilin B present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human Cyclophilin B antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human Cyclophilin B bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": "86 - 137 %", "average": "= 118.7" }, { "sample": "Plasma", "range": "75 - 143 %", "average": "= 109.4" }, { "sample": "Cell culture media", "range": "89 - 144 %", "average": "= 119.3" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab277409-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB277409-CMP04", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB277409-CMP02", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB277409-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human Cyclophilin B coated Microplate", "number":"AB277409-CMP01", "productcode":"" }, { "size":"1 x 200 µL", "name":"700X HRP-Streptavidin Concentrate", "number":"AB277409-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB277409-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human Cyclophilin B Antibody", "number":"AB277409-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Cyclophilin B standard protein (Lyophilized)", "number":"AB277409-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding.
See full target information PPIB
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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