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AB213772

Human CYR61/CCN1 ELISA Kit

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Human CYR61/CCN1 ELISA Kit is a Sandwich ELISA for the measurement of Human CYR61/CCN1 in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

CYR61, GIG1, IGFBP10, CCN1, CCN family member 1, Cellular communication network factor 1, Cysteine-rich angiogenic inducer 61, Insulin-like growth factor-binding protein 10, Protein CYR61, Protein GIG1, IBP-10, IGF-binding protein 10, IGFBP-10

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Sandwich ELISA - Human CYR61/CCN1 ELISA Kit (AB213772)
  • sELISA

Supplier Data

Sandwich ELISA - Human CYR61/CCN1 ELISA Kit (AB213772)

Human CYR61 ELISA Kit (CCN1) (ab213772) Standard Curve.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, EDTA Plasma, Heparin Plasma, Milk, Saliva, Serum, Urine

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 10 pg/mL

Range

31.2 - 2000 pg/mL

Assay time

3h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

The Human CYR61/CCN1 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213772) is designed for the quantitative measurement of Human CYR61/CCN1 in cell culture supernatants, serum, plasma (heparin, EDTA), saliva, urine and human milk.

The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from mouse specific for CYR61/CCN1 has been pre-coated onto 96-well plates. Standards (Expression system for standard: CHO; Immunogen sequence: A22-D381) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CYR61/CCN1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human CYR61/CCN1 amount of sample captured in plate.

Cysteine-rich angiogenic inducer 61 (CYR61) or CCN family member 1 (CCN1), is a matricellular protein that in humans is encoded by the CYR61 gene. This gene is mapped to 1p22.3. CYR61 is capable of regulating a broad range of cellular activities, including cell adhesion, migration, proliferation, differentiation, apoptosis and senescence through interaction with cell surface integrin receptors and heparan sulfate proteoglycans. During embryonic development, CYR61 is critical for cardiac septal morphogenesis, blood vessel formation in placenta and vascular integrity. In adulthood CYR61 plays important roles in inflammation and tissue repair and is associated with diseases related to chronic inflammation, including rheumatoid arthritis, atherosclerosis, diabetes related nephropathy and retinopathy, and many different forms of cancers.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 16, "mean": "814 pg/mL", "standardDeviation": "55.35", "coefficientOfVariability": "= 6.8" }, { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 16, "mean": "248 pg/mL", "standardDeviation": "16.12", "coefficientOfVariability": "= 6.5" }, { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 24, "mean": "806 pg/mL", "standardDeviation": "64.48", "coefficientOfVariability": "= 8" }, { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 24, "mean": "242 pg/mL", "standardDeviation": "17.66", "coefficientOfVariability": "= 7.3" }, { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 16, "mean": "61 pg/mL", "standardDeviation": "2.86", "coefficientOfVariability": "= 4.7" }, { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 24, "mean": "59 pg/mL", "standardDeviation": "3.3", "coefficientOfVariability": "= 5.6" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab213772-1x96Tests", "assetComponentDetails": [ { "size":"1 x 100 µL", "name":"Avidin-Biotin-Peroxidase Complex (ABC)", "number":"AB213772-CMP10", "productcode":"" }, { "size":"1 x 100 µL", "name":"Biotinylated anti- Human CYR61/CCN1 antibody", "number":"AB213772-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human CYR61/CCN1 coated Microplate (12 x 8 wells)", "number":"AB213772-CMP08", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Color Developing Agent", "number":"AB213772-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Antibody Diluent Buffer", "number":"AB213772-CMP09", "productcode":"" }, { "size":"1 x 30 mL", "name":"Sample Diluent Buffer", "number":"AB213772-CMP02", "productcode":"" }, { "size":"4 x 1 Unit", "name":"Adhesive Plate Seal", "number":"AB213772-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized recombinant Human CYR61/CCN1 standard", "number":"AB213772-CMP01", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Stop Solution", "number":"AB213772-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"Wash Buffer (25X)", "number":"AB213772-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"ABC Diluent Buffer", "number":"AB213772-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The CYR61/CCN1 protein also known simply as CYR61 CCN1 or CYR61 protein is a cellular protein with a molecular weight of approximately 40 kDa. It is a member of the CCN family of regulatory proteins which includes connective tissue growth factors. CYR61 is secreted into the extracellular matrix and has a critical role in cell adhesion and signaling. The protein is expressed in various tissues with higher levels observed in the placenta lungs and kidneys reflecting its role in organ development and repair processes.
Biological function summary

The CYR61/CCN1 protein acts as a signaling molecule that influences cell proliferation migration and adhesion. It interacts with integrins and heparan sulfate proteoglycans on the cell surface facilitating cellular communication that is essential for structural and functional tissue integrity. Additionally CYR61 is a part of multiprotein complexes impacting angiogenesis and inflammation. These activities place CYR61/CCN1 at a pivotal point in wound healing and vascularization processes.

Pathways

The CYR61/CCN1 protein participates in key signaling pathways such as the MAPK/ERK and Wnt pathways. These pathways modulate gene expression related to cell growth and differentiation. CYR61 collaborates with other proteins including VEGF and FGF to regulate cardiovascular development and morphogenesis. The interaction with these pathways highlights the protein’s integral role in cellular homeostasis and response to physiological changes.

CYR61/CCN1 is linked to cancer progression and fibrotic diseases. Elevated levels of this protein are frequently found in breast cancer emphasizing its role in tumor growth and metastasis. It associates with other proteins like TGF-beta during fibrotic responses contributing to the pathology of fibrosis in organs such as the liver and lungs. The involvement in these processes highlights its potential as a therapeutic target in oncology and fibrotic disease management.
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Product protocols

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Target data

Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CCN1-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3.
See full target information CCN1
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websiteProtocolBooklet
en
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Product promise

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