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AB238267

Human CYR61 ELISA Kit

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Human CYR61 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CYR61 with a sensitivity of 5 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CYR61, GIG1, IGFBP10, CCN1, CCN family member 1, Cellular communication network factor 1, Cysteine-rich angiogenic inducer 61, Insulin-like growth factor-binding protein 10, Protein CYR61, Protein GIG1, IBP-10, IGF-binding protein 10, IGFBP-10

8 Images
Sandwich ELISA - Human CYR61 ELISA Kit (AB238267)
  • sELISA

Supplier Data

Sandwich ELISA - Human CYR61 ELISA Kit (AB238267)

Example of human CYR61 standard curve in Sample Diluent 25BP.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human CYR61 ELISA Kit (AB238267)
  • sELISA

Supplier Data

Sandwich ELISA - Human CYR61 ELISA Kit (AB238267)

Human CYR61 Standard Curve Comparison. Standard Curve comparison between Human CYR61 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

Sandwich ELISA - Human CYR61 ELISA Kit (AB238267)
  • sELISA

Supplier Data

Sandwich ELISA - Human CYR61 ELISA Kit (AB238267)

Example of human CYR61 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human CYR61 ELISA Kit (AB238267)
  • sELISA

Supplier Data

Sandwich ELISA - Human CYR61 ELISA Kit (AB238267)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CYR61 concentration was determined to be 170.3 pg/mL with a range of 97. 07 – 254.9 pg/mL.

Sandwich ELISA - Human CYR61 ELISA Kit (AB238267)
  • sELISA

Supplier Data

Sandwich ELISA - Human CYR61 ELISA Kit (AB238267)

Interpolated concentrations of native CYR61 in human HeLa cell extract based on a 250 μg/mL extract load and MDA-MB435S cell extract based on a 500 μg/mL extract load. The concentrations of CYR61 were measured in duplicate and interpolated from the CYR61 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CYR61 concentration was determined to be 1,097 pg/mL in HeLa cell extract and 1,717 pg/mL in MDA-MB-435S cell extract.

Sandwich ELISA - Human CYR61 ELISA Kit (AB238267)
  • sELISA

Supplier Data

Sandwich ELISA - Human CYR61 ELISA Kit (AB238267)

Example of human CYR61 standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human CYR61 ELISA Kit (AB238267)
  • sELISA

Supplier Data

Sandwich ELISA - Human CYR61 ELISA Kit (AB238267)

Interpolated concentrations of native CYR61 in human serum and plasma samples. The concentrations of CYR61 were measured in duplicates, interpolated from the CYR61 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 100%, plasma (citrate) 100%, and plasma (EDTA) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CYR61 concentration was determined to be 182.2 pg/mL in neat serum, 123.5 pg/mL in neat plasma (citrate), and 155.3 pg/mL in neat plasma (EDTA).

Sandwich ELISA - Human CYR61 ELISA Kit (AB238267)
  • sELISA

Supplier Data

Sandwich ELISA - Human CYR61 ELISA Kit (AB238267)

Interpolated concentrations of native CYR61 in human breast milk (de-fatted), saliva, and cell culture supernatant (SN) samples.

The concentrations of CYR61 were measured in duplicates, interpolated from the CYR61 standard curves and corrected for sample dilution. Undiluted samples are as follows : milk 100%, saliva 100%, HeLa supernatant 25%, and MDA-MB-453S 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CYR61 concentration was determined to be 1,432 pg/mL in neat milk, 798.2 pg/mL in neat saliva, 8,357 pg/mL neat HeLa cell culture supernatant, and 1,984 pg/mL in neat MDA-MB-453S cell culture supernatant.

Key facts

Detection method

Colorimetric

Sample types

Saliva, Urine, Cell culture extracts, Tissue Extracts, Citrate plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 5 pg/mL

Range

31.3 - 3000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human CYR61 ELISA Kit (ab238267) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CYR61 protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, milk, saliva, serum, tissue extracts, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CYR61 with 5 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.8" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.5" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "94 - 103 %", "average": "= 100" }, { "sample": "Serum", "range": "108 - 115 %", "average": "= 112" }, { "sample": "Milk", "range": "93 - 96 %", "average": "= 95" }, { "sample": "EDTA Plasma", "range": "84 - 86 %", "average": "= 85" }, { "sample": "Cell culture extracts", "range": "80 - 85 %", "average": "= 83" }, { "sample": "Urine", "range": "103 - 113 %", "average": "= 107" }, { "sample": "Saliva", "range": "110 - 119 %", "average": "= 114" }, { "sample": "Tissue Extracts", "range": "80 - 88 %", "average": "= 84" }, { "sample": "Citrate plasma", "range": "86 - 96 %", "average": "= 92" }, { "sample": "Cell culture media", "range": "89 - 96 %", "average": "= 91" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab238267-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB238267-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB238267-CMP13", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB238267-CMP07", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB238267-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB238267-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB238267-CMP12", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CYR61 Capture Antibody", "number":"AB238267-CMP09", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample Diluent 25BP", "number":"AB238267-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human CYR61 Lyophilized Recombinant Protein", "number":"AB238267-CMP11", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB238267-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB238267-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CYR61 Detector Antibody", "number":"AB238267-CMP10", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB238267-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CYR61 also known as CCN1 is a secreted protein involved in cell signaling. It belongs to the CCN family of proteins and weighs approximately 42 kDa. This protein is found in various tissues including the skin lungs and blood vessels. CYR61 is involved in cellular activities such as adhesion migration and proliferation by interacting with cell surface receptors. This active participation in cell signaling makes CYR61 essential to multiple cellular processes.
Biological function summary

CYR61 supports angiogenesis and wound healing by promoting cell survival and adhesion. It functions as a significant component of the extracellular matrix and often participates in complex formations with other CCN proteins. These complexes aid in mediating interactions between cells and their environments. Through these mechanisms CYR61 influences various biological outcomes impacting how tissues grow and repair.

Pathways

CYR61 contributes to both the integrin and TGF-beta signaling pathways. In the integrin pathway it interacts with proteins like integrin alphaVbeta3 to influence cell movement and survival. The TGF-beta pathway involves its regulation of cellular responses to growth factors. By playing important roles in these pathways CYR61 maintains essential cellular functions and intercellular communication impacting diverse physiological processes.

CYR61 has links to cancer and fibrosis. Its overexpression can contribute to tumor progression and metastasis often in connection with matrix metalloproteinases during cancer development. In fibrosis CYR61 collaborates with fibroblast growth factor receptors to promote tissue scarring and stiffness. These connections highlight the importance of CYR61 in pathological conditions offering potential targets for therapeutic intervention.
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Product protocols

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Target data

Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CCN1-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3.
See full target information CCN1
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websiteProtocolBooklet
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