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AB326749

Human CYR61 ELISA Kit, Chemiluminescent

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Human CYR61 ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human CYR61 with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 16.46 - 4000 pg/ml with a sensitivity of 5 pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending

View Alternative Names

CYR61, GIG1, IGFBP10, CCN1, CCN family member 1, Cellular communication network factor 1, Cysteine-rich angiogenic inducer 61, Insulin-like growth factor-binding protein 10, Protein CYR61, Protein GIG1, IBP-10, IGF-binding protein 10, IGFBP-10

6 Images
Sandwich ELISA - Human CYR61 ELISA Kit, Chemiluminescent (AB326749)
  • sELISA

Lab

Sandwich ELISA - Human CYR61 ELISA Kit, Chemiluminescent (AB326749)

Dynamic range comparison between the Chemiluminescent Human CYR61 SimpleStep ELISA and colorimetric Human CYR61 SimpleStep ELISA (ab238267) for 1X Cell Extraction Buffer PTR, and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Sandwich ELISA - Human CYR61 ELISA Kit, Chemiluminescent (AB326749)
  • sELISA

Lab

Sandwich ELISA - Human CYR61 ELISA Kit, Chemiluminescent (AB326749)

Standard curve comparison between the Chemiluminescent Human CYR61 SimpleStep ELISA and colorimetric Human CYR61 SimpleStep ELISA (ab238267) in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human CYR61 ELISA Kit, Chemiluminescent (AB326749)
  • sELISA

Lab

Sandwich ELISA - Human CYR61 ELISA Kit, Chemiluminescent (AB326749)

Interpolated concentration of native CYR61 was measured in duplicate at different sample concentrations. Undiluted samples are 100% serum, plasma (citrate), and urine. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions for serum and plasma are made in Sample Diluent 25BP, and sample dilutions for urine and made in Sample Diluent NS.

Sandwich ELISA - Human CYR61 ELISA Kit, Chemiluminescent (AB326749)
  • sELISA

Supplier Data

Sandwich ELISA - Human CYR61 ELISA Kit, Chemiluminescent (AB326749)

Interpolated concentrations of native CYR61 in human HeLa cell extract based on a 250 μg/mL extract load and MDA-MB435S cell extract based on a 500 μg/mL extract load. The concentrations of CYR61 were measured in duplicate and interpolated from the CYR61 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CYR61 concentration was determined to be 1,097 pg/mL in HeLa cell extract and 1,717 pg/mL in MDA-MB-435S cell extract.

Sandwich ELISA - Human CYR61 ELISA Kit, Chemiluminescent (AB326749)
  • sELISA

Supplier Data

Sandwich ELISA - Human CYR61 ELISA Kit, Chemiluminescent (AB326749)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CYR61 concentration was determined to be 170.3 pg/mL with a range of 97. 07 – 254.9 pg/mL.

Sandwich ELISA - Human CYR61 ELISA Kit, Chemiluminescent (AB326749)
  • sELISA

Supplier Data

Sandwich ELISA - Human CYR61 ELISA Kit, Chemiluminescent (AB326749)

Interpolated concentrations of native CYR61 in human breast milk (de-fatted), saliva, and cell culture supernatant (SN) samples.

The concentrations of CYR61 were measured in duplicates, interpolated from the CYR61 standard curves and corrected for sample dilution. Undiluted samples are as follows : milk 100%, saliva 100%, HeLa supernatant 25%, and MDA-MB-453S 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CYR61 concentration was determined to be 1,432 pg/mL in neat milk, 798.2 pg/mL in neat saliva, 8,357 pg/mL neat HeLa cell culture supernatant, and 1,984 pg/mL in neat MDA-MB-453S cell culture supernatant.

Key facts

Detection method

Luminescent

Sample types

Cell culture supernatant, Serum, Milk, Cell culture extracts, Urine, Saliva, Tissue Extracts, Citrate plasma

Reacts with

Human, Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 5 pg/mL

Range

16.46 - 4000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

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Product details

Human CYR61 ELISA Kit, Chemiluminescent (ab326749) is a rapid single-wash sandwich 55-min ELISA to measure Human CYR61 in Cell culture supernatant, Serum, Milk, Cell culture extracts, Urine, Saliva, Tissue Extracts, Citrate plasma with a sensitivity of 5 pg/mL.

How the assay works

Human CYR61 ELISA Kit, Chemiluminescent (ab326749) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human CYR61 (ab326749) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-CCN1 antibody [EPR21133-104] - BSA and Aside free (Capture) ab244801
- Anti-CCN1 antibody [EPR21133-20] - BSA and Aside free (Detector) ab245074
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Precision

[ { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2.5" }, { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.8" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "94 - 103 %", "average": "= 103" }, { "sample": "Serum", "range": "108 - 115 %", "average": "= 115" }, { "sample": "Milk", "range": "93 - 96 %", "average": "= 96" }, { "sample": "Cell culture extracts", "range": "80 - 85 %", "average": "= 85" }, { "sample": "Urine", "range": "103 - 113 %", "average": "= 113" }, { "sample": "Saliva", "range": "110 - 119 %", "average": "= 119" }, { "sample": "Tissue Extracts", "range": "80 - 88 %", "average": "= 88" }, { "sample": "Citrate plasma", "range": "86 - 96 %", "average": "= 96" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CCN1-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3.
See full target information CCN1
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com