JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB283973

Human Cystatin A ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Human Cystatin A ELISA Kit is a Sandwich ELISA for the measurement of Human Cystatin A in Human in Biofluids, Cell/Tissue Extracts samples.
1 Images
Sandwich ELISA - Human Cystatin A ELISA Kit (AB283973)
  • sELISA

Supplier Data

Sandwich ELISA - Human Cystatin A ELISA Kit (AB283973)

Example of Human Cystatin A standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Urine, Plasma, Serum, Cell Lysate, Tissue

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 17 pg/mL

Range

0.031 - 2 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Complementary Products

Primary Antibodies

AB128948

Anti-Cystatin A antibody [EPR6941]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-Cystatin A antibody [EPR6941] (AB128948)

  • 0
  • 0
Proteins & Peptides

AB265857

Recombinant human Cystatin A protein (Active) (His tag N-Terminus)

Functional Studies - Recombinant human Cystatin A protein (Active) (His tag N-Terminus) (AB265857)

  • 0
  • 0
Assay Kits

AB284614

Human ABCA1 ELISA Kit

Recombinant|SimpleStep

ELISA - Human ABCA1 ELISA Kit (AB284614)

  • 0
  • 0
Proteins & Peptides

AB275986

Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein RBD (His tag)

Indirect ELISA - Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein RBD (His tag) (AB275986)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

The Human Cystatin-A ELISA (Enzyme-Linked Immunosorbent Assay) Kit (ab283973) is designed for detection of Cystatin-Ain human plasma, serum, urine, and cell culture samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures human Cystatin-A in approximately 4 hours.

A polyclonal antibody specific for human Cystatin-A has been pre-coated onto a 96-well microplate with removable strips. Cystatin-A in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human Cystatin-A, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

style
left-column

Precision & recovery information

Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 60, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 60, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.7" } ]

style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab283973-1x96Tests", "assetComponentDetails": [ { "size":"1 x 30 mL", "name":"10X Diluent N Concentrate", "number":"AB283973-CMP01", "productcode":"" }, { "size":"1 x 2 mL", "name":"1X Standard Diluent", "number":"AB283973-CMP02", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB283973-CMP09", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB283973-CMP04", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB283973-CMP08", "productcode":"" }, { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB283973-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human Cystatin A Microplate", "number":"AB283973-CMP05", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB283973-CMP10", "productcode":"" }, { "size":"1 x 120 µL", "name":"50X Biotinylated Human Cystatin A Antibody", "number":"AB283973-CMP07", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Cystatin A Standard", "number":"AB283973-CMP03", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
style
left-column
style
left-column

Product protocols

style
left-column

Target data

This is an intracellular thiol proteinase inhibitor. Has an important role in desmosome-mediated cell-cell adhesion in the lower levels of the epidermis.
See full target information CSTA

Alternative Names

STF1, STFA, CSTA, Cystatin-A, Cystatin-AS, Stefin-A
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com