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AB313683

Human Cystatin A ELISA Kit

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Human Cystatin A ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Cystatin A in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

STF1, STFA, CSTA, Cystatin-A, Cystatin-AS, Stefin-A

1 Images
Sandwich ELISA - Human Cystatin A ELISA Kit (AB313683)
  • sELISA

Supplier Data

Sandwich ELISA - Human Cystatin A ELISA Kit (AB313683)

Example of standard curve using ab313683. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 6.5 pg/mL

Range

6.5 - 1500 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

Product details

Human Cystatin A ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Cystatin A in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human Cystatin A coated on a 96-well plate.

This ELISA kit shows no cross-reactivity with the following cytokines tested: human ADAM12, ADAM-8, B7-H3 (CD276), BMPR-IB, Cadherin-13, Cadherin-4, CD155 (PVR), CD229 (SLAMF3), CD48 (SLAMF2), CD58 (LFA-3), CD84 (SLAMF5), CD99, CEACAM-5, Coagulation Factor XIV (Protein C), Cystatin B, Cystatin E/M, Desmoglein-2, DR3 (TNFRSF25), ErbB4 (HER4), ESAM, FGF-21, Galectin-2, Galectin-9, ICOS, JAM-A (CD321/F11R), JAM-B (VE-JAM/CD322), Kallikrein 5, Midkine, Pentraxin-3 (TSG-14), Pref-1 (DLK-1/FA1), Siglec-10, SLAM (CD150), SP-D, Syndecan-4, Testican 2 (SPOCK2), TIM-3 (KIM-3), TLR4, TRAIL (TNFSF10), ULBP-1.

Recovery

[ { "sample": "Serum", "range": "107 - 145 %", "average": "= 124.2" }, { "sample": "Plasma", "range": "88 - 113 %", "average": "= 100.3" }, { "sample": "Cell culture media", "range": "81 - 113 %", "average": "= 98.13" } ]

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab313683-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Human Cystatin A Antibody-coated ELISA Plate", "number":"AB313683-CMP08", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin", "number":"AB313683-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human Cystatin A Protein Standard", "number":"AB313683-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB313683-CMP07", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB313683-CMP04", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB313683-CMP05", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB313683-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human Cystatin A Detection Antibody", "number":"AB313683-CMP09", "productcode":"" } ] } } }

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Product protocols

Target data

This is an intracellular thiol proteinase inhibitor. Has an important role in desmosome-mediated cell-cell adhesion in the lower levels of the epidermis.
See full target information CSTA
websiteProtocolBooklet
en

Product promise

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