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Human Cystatin C ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human Cystatin C in Human in Cell culture supernatant, EDTA Plasma, Heparin Plasma, Milk, Serum, Urine samples.

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Sandwich ELISA - Human Cystatin C ELISA Kit (AB119589), expandable thumbnail

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Key facts

Detection method
Colorimetric
Sample types
Cell culture supernatant, EDTA Plasma, Heparin Plasma, Milk, Serum, Urine
Assay type
Sandwich (quantitative)
Reactive species
Human
Range
0.312 - 20 ng/mL
Sensitivity
< 10 pg/mL

Reactivity data

Application
sELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Target data

Function

As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.

Alternative names

Cystatin-C, Cystatin-3, Gamma-trace, Neuroendocrine basic polypeptide, Post-gamma-globulin, CST3

What's included?

1 x 96 Tests
Components
ABC Diluent Buffer
1 x 12 mL
Anti-Human Cystatin C antibody Microplate (12 x 8 wells)
1 x 1 Unit
Antibody Diluent Buffer
1 x 12 mL
Avidin-Biotin-Peroxidase Complex (ABC)
1 x 100 µL
Biotinylated anti- Human Cystatin C antibody
1 x 100 µL
Lyophilized recombinant Human Cystatin C standard
2 x 20 ng
Plate Seal
4 x 1 Unit
Sample Diluent Buffer
1 x 30 mL
TMB Color Developing Agent
1 x 10 mL
TMB Stop Solution
1 x 10 mL
Wash Buffer (25X)
1 x 20 mL

Recommended products

Human Cystatin C ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human Cystatin C in Human in Cell culture supernatant, EDTA Plasma, Heparin Plasma, Milk, Serum, Urine samples.

Key facts

Detection method
Colorimetric
Sample types
Cell culture supernatant, EDTA Plasma, Heparin Plasma, Milk, Serum, Urine
Assay type
Sandwich (quantitative)
Reactive species
Human
Range
0.312 - 20 ng/mL
Assay Platform
Microplate
Sensitivity
< 10 pg/mL

Precision

Intra assay

Sample
Sample 3
n
16
mean
10.374 ng/mL
SD
0.684
C.V.
= 6.6
Sample
Sample 2
n
16
mean
2.837 ng/mL
SD
0.207
C.V.
= 7.3
Sample
Sample 1
n
16
mean
0.687 ng/mL
SD
0.044
C.V.
= 6.4

Inter assay

Sample
Sample 2
n
24
mean
2.715 ng/mL
SD
0.217
C.V.
= 8
Sample
Sample 3
n
24
mean
10.112 ng/mL
SD
0.859
C.V.
= 8.5
Sample
Sample 1
n
24
mean
0.647 ng/mL
SD
0.051
C.V.
= 7.9

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

Abcam's Human Cystatin C in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human Cystatin C in cell culture supernatants, serum, plasma (heparin, EDTA) saliva, urine and Human milk.

A Cystatin C specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for Cystatin C is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human Cystatin C amount of sample captured in plate.

Get results in 90 minutes with Human Cystatin C ELISA Kit (ab179883) from our SimpleStep ELISA® range.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Sandwich ELISA - Human Cystatin C ELISA Kit (ab119589), expandable thumbnail

    Sandwich ELISA - Human Cystatin C ELISA Kit (ab119589)

    Representative Standard Curve using 119589.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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