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AB119589

Human Cystatin C ELISA Kit

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(3 Publications)

Human Cystatin C ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Cystatin C in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

Cystatin-C, Cystatin-3, Gamma-trace, Neuroendocrine basic polypeptide, Post-gamma-globulin, CST3

1 Images
Sandwich ELISA - Human Cystatin C ELISA Kit (AB119589)
  • sELISA

Supplier Data

Sandwich ELISA - Human Cystatin C ELISA Kit (AB119589)

Representative Standard Curve using 119589.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, EDTA Plasma, Heparin Plasma, Milk, Serum, Urine

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 10 pg/mL

Range

0.312 - 20 ng/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Human Cystatin C in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human Cystatin C in cell culture supernatants, serum, plasma (heparin, EDTA) saliva, urine and Human milk.

A Cystatin C specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for Cystatin C is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human Cystatin C amount of sample captured in plate.

Get results in 90 minutes with Human Cystatin C ELISA Kit (ab179883) from our SimpleStep ELISA® range.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 24, "mean": "2.715 ng/mL", "standardDeviation": "0.217", "coefficientOfVariability": "= 8" }, { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 24, "mean": "10.112 ng/mL", "standardDeviation": "0.859", "coefficientOfVariability": "= 8.5" }, { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 16, "mean": "10.374 ng/mL", "standardDeviation": "0.684", "coefficientOfVariability": "= 6.6" }, { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 24, "mean": "0.647 ng/mL", "standardDeviation": "0.051", "coefficientOfVariability": "= 7.9" }, { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 16, "mean": "2.837 ng/mL", "standardDeviation": "0.207", "coefficientOfVariability": "= 7.3" }, { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 16, "mean": "0.687 ng/mL", "standardDeviation": "0.044", "coefficientOfVariability": "= 6.4" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab119589-1x96Tests", "assetComponentDetails": [ { "size":"1 x 30 mL", "name":"Sample Diluent Buffer", "number":"AB119589-CMP02", "productcode":"" }, { "size":"1 x 100 µL", "name":"Biotinylated anti- Human Cystatin C antibody", "number":"AB119589-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human Cystatin C antibody Microplate (12 x 8 wells)", "number":"AB119589-CMP10", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Color Developing Agent", "number":"AB119589-CMP04", "productcode":"" }, { "size":"1 x 100 µL", "name":"Avidin-Biotin-Peroxidase Complex (ABC)", "number":"AB119589-CMP09", "productcode":"" }, { "size":"4 x 1 Unit", "name":"Plate Seal", "number":"AB119589-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"Antibody Diluent Buffer", "number":"AB119589-CMP06", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Stop Solution", "number":"AB119589-CMP05", "productcode":"" }, { "size":"2 x 20 ng", "name":"Lyophilized recombinant Human Cystatin C standard", "number":"AB119589-CMP08", "productcode":"" }, { "size":"1 x 20 mL", "name":"Wash Buffer (25X)", "number":"AB119589-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"ABC Diluent Buffer", "number":"AB119589-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.
See full target information CST3
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Asia Pacific allergy 13:158-163 PubMed38094096

2023

Activities and concentration of alpha-1 antitrypsin and cystatin C in serum from patients with house dust mite asthma.

Applications

Unspecified application

Species

Unspecified reactive species

Mailani Dwi Hidayati,Febriana Catur Iswanti,Samsuridjal Djauzi,Sukamto Koesnoe,Mohamad Sadikin

Pharmacogenomics and personalized medicine 16:481-489 PubMed37256202

2023

Plasma Homocysteine (Hcy) Concentration Functions as a Predictive Biomarker of SPECT-Evaluated Post-Ischemic Hyperperfusion in Acute Ischemic Stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Yingqiu Wang,Renhua Hou,Yan Liu

Sensors (Basel, Switzerland) 21: PubMed34063596

2021

Development and Evaluation of a Quantitative Fluorescent Lateral Flow Immunoassay for Cystatin-C, a Renal Dysfunction Biomarker.

Applications

Unspecified application

Species

Unspecified reactive species

Satheesh Natarajan,Maria C DeRosa,Malay Ilesh Shah,Joseph Jayaraj
View all publications
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Product promise

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