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AB314723

Human Cystatin D ELISA Kit

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Human Cystatin D ELISA Kit is a Sandwich ELISA for the measurement of Human Cystatin D in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

Cystatin-D, Cystatin-5, CST5

1 Images
Sandwich ELISA - Human Cystatin D ELISA Kit (AB314723)
  • sELISA

Supplier Data

Sandwich ELISA - Human Cystatin D ELISA Kit (AB314723)

Example of standard curve using ab314723. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.1 ng/mL

Range

0.102 - 25 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

Product details

Human Cystatin D ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Cystatin D in serum (human Cystatin D concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants. This assay employs an antibody specific for human Cystatin D coated on a 96-well plate.

Recovery

[ { "sample": "Serum", "range": "139 - 149 %", "average": "= 143.6" }, { "sample": "Plasma", "range": "128 - 148 %", "average": "= 138.5" }, { "sample": "Cell culture media", "range": "139 - 147 %", "average": "= 144.3" } ]

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab314723-1x96Tests", "assetComponentDetails": [ { "size":"2 x 1 Vial", "name":"Lyophilized Human Cystatin D Protein Standard", "number":"AB314723-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human Cystatin D Detection Antibody", "number":"AB314723-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human Cystatin D Antibody-coated ELISA Plate", "number":"AB314723-CMP08", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB314723-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"500X HRP-Streptavidin", "number":"AB314723-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB314723-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB314723-CMP07", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB314723-CMP06", "productcode":"" } ] } } }

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Product protocols

Target data

Cysteine proteinase inhibitor that possibly plays a protective role against proteinases present in the oral cavity. The order of preference for inhibition is cathepsin S > cathepsin H > cathepsin L > cathepsin B.
See full target information CST5
websiteProtocolBooklet
en

Product promise

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