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AB313684

Human Cystatin F ELISA Kit

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Human Cystatin F ELISA Kit is a Sandwich ELISA for the measurement of Human Cystatin F in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

Cystatin-F, Cystatin-7, Cystatin-like metastasis-associated protein, Leukocystatin, CMAP, CST7

1 Images
Sandwich ELISA - Human Cystatin F ELISA Kit (AB313684)
  • sELISA

Supplier Data

Sandwich ELISA - Human Cystatin F ELISA Kit (AB313684)

Example of standard curve using ab313684. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 2 pg/mL

Range

2 - 400 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

Product details

Human Cystatin F ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Cystatin F in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human Cystatin F coated on a 96-well plate.

Recovery

[ { "sample": "Serum", "range": "126 - 142 %", "average": "= 132.4" }, { "sample": "Plasma", "range": "116 - 134 %", "average": "= 124.1" }, { "sample": "Cell culture media", "range": "108 - 133 %", "average": "= 116.6" } ]

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab313684-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB313684-CMP04", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB313684-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB313684-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human Cystatin F Protein Standard", "number":"AB313684-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human Cystatin F Antibody-coated ELISA Plate", "number":"AB313684-CMP08", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB313684-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"500X HRP-Streptavidin", "number":"AB313684-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human Cystatin F Detection Antibody", "number":"AB313684-CMP09", "productcode":"" } ] } } }

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Product protocols

Target data

Inhibits papain and cathepsin L but with affinities lower than other cystatins. May play a role in immune regulation through inhibition of a unique target in the hematopoietic system.
See full target information Cystatin-F
websiteProtocolBooklet
en

Product promise

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