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AB314724

Human Cystatin SA/CST2 2 ELISA Kit

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Human Cystatin SA/CST2 2 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Cystatin SA/CST2 2 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

Cystatin-SA, Cystatin-2, Cystatin-S5, CST2

1 Images
Sandwich ELISA - Human Cystatin SA/CST2 2 ELISA Kit (AB314724)
  • sELISA

Supplier Data

Sandwich ELISA - Human Cystatin SA/CST2 2 ELISA Kit (AB314724)

Example of standard curve using ab314724. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.38 ng/mL

Range

0.38 - 100 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Cystatin SA/CST2 2 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Cystatin SA/CST2 2 in serum (human Cystatin SA/CST2 2 concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants. This assay employs an antibody specific for human Cystatin SA coated on a 96-well plate.

This ELISA kit shows no cross-reactivity with the following cytokines tested: human ADAMTSL-1/Punctin-1, AMSH/STAMBP, Annexin V, BATF3, Bora, Cadherin-17, Caveolin-2, CD2, CD200 R1, CHST3, COMT, Dopamine beta-Hydroxylase, Desmin,Exostosin-like 3, Ficolin-1, FosB/GOS3, FRS2, GATA-5, GFAP, GLI-3, HepaCAM, ARNT/HIF-1 beta, 17 betaHSD1/HSD17B1, IDO, Kallikrein 1, Kell, MDL-1/CLEC5A, NPDC-1, Numb, Olig2, p63/TP73L, Pax3, Semaphorin 4D, Sphingosine Kinase 1/SPHK1, TAZ/WWTR1, TC-PTP, Transglutaminase 3/TGM3, TPST2, TREML1/TLT-1.

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Recovery

[ { "sample": "Serum", "range": "79 - 91 %", "average": "= 85.35" }, { "sample": "Plasma", "range": "75 - 105 %", "average": "= 87.14" }, { "sample": "Cell culture media", "range": "90 - 92 %", "average": "= 91.08" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cystatin SA also known as CST2 is a member of the cystatin superfamily which are cysteine protease inhibitors. It has a molecular mass of approximately 16 kilodaltons. This protein is expressed mainly in the human saliva and tears where it helps to protect the oral cavity and ocular surface by inhibiting protease activity. The primary role of Cystatin SA is to bind tightly to its target cysteine proteases preventing them from degrading proteins in these sensitive tissues.
Biological function summary

Cystatin SA plays an important role in maintaining tissue integrity and homeostasis. It is not known to be part of a complex but operates individually to inhibit cysteine proteases like cathepsin B H and L. By controlling the proteolytic activities of these enzymes Cystatin SA helps to prevent excessive tissue breakdown and inflammation which contributes to the preservation of mucosal barriers in the body.

Pathways

Cystatin SA's inhibitory action plays a significant role in proteolysis and inflammation pathways. One important pathway involves cathepsins which are targeted by Cystatin SA to modulate their activity. Another related pathway includes tissue remodeling where Cystatin SA helps regulate protease activities to maintain structural balance. Both these pathways emphasize its interaction with similar cystatins such as Cystatin C in modulating protease activities to protect tissues.

Cystatin SA has been implicated in conditions such as periodontitis and dry eye syndrome. In periodontitis altered Cystatin SA levels can affect the proteolytic balance contributing to tissue destruction and inflammation. For dry eye syndrome its role in protecting ocular surfaces brings it into focus as a protective factor. Changes in Cystatin SA expression are often correlated with such conditions and it connects with other proteins like cathepsin L which is involved in tissue damage in these disorders.
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Product protocols

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Target data

Thiol protease inhibitor.
See full target information CST2
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websiteProtocolBooklet
en
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Product promise

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