Human Cyt C Reductase ELISA Kit (Complex III) Profiling ELISA Kit is a Sandwich (qualitative) ELISA kit for the measurement of Human Cyt C Reductase (Complex III) Profiling ELISA Kit in Human in Tissue Extracts, Cell Lysate samples.
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- Pharmaceutics 14:20223D Spheroids of Human Primary Urine-Derived Stem Cells in the Assessment of Drug-Induced Mitochondrial Toxicity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuifen Ding,Kalyani Jambunathan,Guochun Jiang,David M Margolis,Iris Leng,Michael Ihnat,Jian-Xing Ma,Jon Mirsalis,Yuanyuan ZhangPubMed 35631624
- Molecular therapy : the journal of the American Society of Gene Therapy 30:714-7252021Downregulation of a mitochondrial micropeptide, MPM, promotes hepatoma metastasis by enhancing mitochondrial complex I activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMan-Huan Xiao,Yi-Fang Lin,Peng-Peng Xie,Hua-Xing Chen,Jun-Wen Deng,Wei Zhang,Na Zhao,Chen Xie,Yu Meng,Xingguo Liu,Shi-Mei Zhuang,Ying Zhu,Jian-Hong FangPubMed 34478872
- Phytotherapy research : PTR 35:3390-34052021Moschamindole induces glioma cell apoptosis by blocking Mia40-dependent mitochondrial intermembrane space assembly and oxidative respiration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuo-Dong Huang,Fan-Fan Chen,Ji-Hu Yang,Guo-Xu Ma,Zi-Jun Liao,Wei-Ping Li,Zong-Yang Li,Lei ChenPubMed 33856743
- Cellular signalling 71:1095982020STAT3 inhibition induced temozolomide-resistant glioblastoma apoptosis via triggering mitochondrial STAT3 translocation and respiratory chain dysfunction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPing Cui et. al.PubMed 32165236
- The journal of physiological sciences : JPS 69:1005-10172019Cross talk between 26S proteasome and mitochondria in human mesenchymal stem cells' ability to survive under hypoxia stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRamada R Khasawneh et. al.PubMed 31679117
- International journal of molecular sciences 20:2019Degradation of Mitochondria and Oxidative Stress as the Main Mechanism of Toxicity of Pristine Graphene on U87 Glioblastoma Cells and Tumors and HS-5 Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSławomir Jaworski et. al.PubMed 30717385
- Frontiers in molecular neuroscience 10:1642017Mitochondrial Effects of PGC-1alpha Silencing in MPP Treated Human SH-SY5Y Neuroblastoma Cells.
- Stem cells (Dayton, Ohio) 31:1287-972013Induced pluripotent stem cells with a mitochondrial DNA deletion.Applications:ELISAReactive species:HumanAnne B C Cherry,Katelyn E Gagne,Erin M McLoughlin,Anna Baccei,Bryan Gorman,Odelya Hartung,Justine D Miller,Jin Zhang,Rebecca L Zon,Tan A Ince,Ellis J Neufeld,Paul H Lerou,Mark D Fleming,George Q Daley,Suneet AgarwalPubMed 23400930
Key facts
- Detection method
Colorimetric
- Sample types
Tissue Extracts, Cell Lysate
- Assay type
Sandwich (qualitative)
- Reactive species
Human
- Range
6 - 200 µg/mL
- Sensitivity
= 4 µg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. The cytochrome b-c1 complex catalyzes electron transfer from ubiquinol to cytochrome c, linking this redox reaction to translocation of protons across the mitochondrial inner membrane, with protons being carried across the membrane as hydrogens on the quinol. In the process called Q cycle, 2 protons are consumed from the matrix, 4 protons are released into the intermembrane space and 2 electrons are passed to cytochrome c (By similarity). The 2 core subunits UQCRC1/QCR1 and UQCRC2/QCR2 are homologous to the 2 mitochondrial-processing peptidase (MPP) subunits beta-MPP and alpha-MPP respectively, and they seem to have preserved their MPP processing properties (By similarity). May be involved in the in situ processing of UQCRFS1 into the mature Rieske protein and its mitochondrial targeting sequence (MTS)/subunit 9 when incorporated into complex III (Probable).
Alternative names
Complex III subunit 2, Core protein II, Ubiquinol-cytochrome-c reductase complex core protein 2, UQCRC2
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Human Cyt C Reductase ELISA Kit (Complex III) Profiling ELISA Kit is a Sandwich (qualitative) ELISA kit for the measurement of Human Cyt C Reductase (Complex III) Profiling ELISA Kit in Human in Tissue Extracts, Cell Lysate samples.
Key facts
- Detection method
Colorimetric
- Sample types
Tissue Extracts, Cell Lysate
- Assay type
Sandwich (qualitative)
- Reactive species
Human
- Range
6 - 200 µg/mL
- Assay Platform
Microplate
- Sensitivity
= 4 µg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 8 | mean - | SD - | C.V. 4.7 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 6 | mean - | SD - | C.V. 19.7 |
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Ubiquinol-cytochrome-c reductase (Complex III) is the third enzyme of the oxidative phosphorylation (OXPHOS) system within the mitochondrial inner membrane. Ubiquinol-cytochrome-c reductase is a large protein complex of approximately 250,000 MW made up of 11 different subunits. The enzyme forms a dimer in the mitochondrial inner membrane. The catalytic subunit cytochrome b is encoded on mitochondrial DNA (mtDNA). All other subunits are encoded by nuclear genomic DNA, made in the cytosol, and translocated into the organelle for assembly at the inner membrane. Complex III catalyses the pumping of protons across the inner membrane and transfer of electrons from ubiquinol to cytochrome c by a mechanism known as the Q cycle in which ubiquinol (Q) the electron carrier exists as a reduced, partially reduced or oxidized form.
ab124537 Cyt C Reductase (Complex III) human profiling kit is an in vitro enzyme-linked immunosorbent assay (ELISA) for the comparison of Complex III levels or profile in cell and tissue lysates. The assay employs a human Complex III specific antibody coated onto microplate well plate strips.
Samples are pipetted into the wells and Complex III present in the sample is bound to the wells by the immobilized antibody. The wells are washed and an anti-Complex III detector antibody is added. After washing away unbound detector antibody, an HRP-conjugated secondary antibody specific for the detector antibody is pipetted into the wells. The wells are again washed, an HRP substrate solution (TMB) is added to the wells and color develops in proportion to the amount of Complex III bound. The developing blue color is measured at 600 nm.
Optionally the reaction can be stopped by adding hydrochloric acid which changes the color from blue to yellow and the intensity can be measured at 450 nm.
Species– Human. Rat and Mouse samples are not appropriate, other species are untested.
Store all components at 4°C. This kit is stable for 6 months from receipt. Unused microplate strips should be returned to the pouch containing the desiccant and resealed.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Complex III also known as cytochrome c reductase or cytochrome bc1 complex plays an important role in the mitochondrial electron transport chain. This multiprotein complex weighing approximately 250 kDa facilitates electron transfer from ubiquinol to cytochrome c. Complex III resides in the inner mitochondrial membrane of eukaryotic cells and is important for cellular energy production. Structurally it consists of 11 subunits in mammals with cytochrome b cytochrome c1 and Rieske iron-sulfur protein being the core components.
- Biological function summary
Complex III transfers electrons and contributes to proton translocation across the mitochondrial membrane. This process generates the proton gradient needed for ATP synthesis via oxidative phosphorylation often referred to as OXPHOS. As a core component of the mitochondrial respiratory chain Complex III interacts closely with other complexes mainly Complexes I and IV to maintain efficient energy conversion.
- Pathways
Complex III is integral to the oxidative phosphorylation pathway and the respiratory electron transport chain. It ensures efficient electron transport working closely with Complexes I II and IV. Cytochrome c acts as an electron carrier between Complex III and Complex IV in this pathway. This coordinated interaction is essential for ATP generation meeting cellular energy demands.
- Associated diseases and disorders
Complex III is linked with mitochondrial disorders and neurodegenerative diseases. Deficiencies or mutations in its components may lead to disorders such as mitochondrial encephalopathy or Leigh syndrome which are associated with impaired energy metabolism. The complex's dysfunction also affects proteins connected to these diseases like ATP synthase due to disrupted energy production. Additionally its role in oxidative stress has implications for conditions like mitotox-related mitochondrial damage.
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Sandwich ELISA - Human Cyt C Reductase ELISA Kit (Complex III) Profiling ELISA Kit (ab124537)
Example control sample curve of serially titrated HepG2 extract in the working range of the assay.
Sandwich ELISA - Human Cyt C Reductase ELISA Kit (Complex III) Profiling ELISA Kit (ab124537)
Human HepG2 cells were cultured in NARTI Zalcitabine (ddC) for 6 days to ensure a significant effect on mitochondrial DNA replication and mitochondrial protein translation, respectively. The drug reduced mitochondrial DNA levels and hence mitochondrial protein expression. As a consequence the assembly of Complexes I, III and IV were severely affected. Note that loss of the two small mitochondrial DNA encoded subunits of Complex V (ATP synthase) does not affect overall assembly. Interestingly an increase in Complex II was induced as a consequence of I, III, IV loss possibly to up regulate mitochondrial citric acid cycle function.
Sandwich ELISA - Human Cyt C Reductase ELISA Kit (Complex III) Profiling ELISA Kit (ab124537)
Human HepG2 cells were cultured in chloramphenicol for 6 days to ensure a significant effect on mitochondrial DNA replication and mitochondrial protein translation, respectively. The antibiotic inhibited mitochondrial protein translation and assembly of Complexes I and IV but had no significant effect on Complex II, III or V.
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