Human Cytochrome C ELISA Kit

• ELISA

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ELISA - Human Cytochrome C ELISA Kit (AB221832)

Example of human Cytochrome C standard curve in 1X Cell Extraction Buffer PTR + Enhancer.

• ELISA

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ELISA - Human Cytochrome C ELISA Kit (AB221832)

Reactivity in human and rat heart tissue extracts.

Other species reactivity was determined by measuring a 20 g/mL extract load of human and rat heart tissue extract samples, interpolating the protein concentrations from the human standard curve, and expressing the interpolated concentrations as a percentage of the protein concentration in the human heart tissue extract. Cross-reactivity was determined to be 100% in rat heart extract. Due to 100% amino acids sequence identity of rat and mouse Cytochrome C, the same cross-reactivity can be assumed for mouse Cytochrome C.

• ELISA

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ELISA - Human Cytochrome C ELISA Kit (AB221832)

Interpolated concentrations of native Cytochrome C in human extract samples. The concentrations of Cytochrome C were measured in three different dilutions in duplicate and interpolated from the Cytochrome C standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted in ng of Cytochrome C per mg of extract (mean +/- SD, n=3). Cytochrome C concentration was determined to be 1716 ng/mg heart tissue extract, 670.1 ng/mg in colon tissue extract, 1689 ng/mg in skeletal muscle tissue extract, 924.2 ng/mg in HepG2 cell extract, 1658 ng/mg in PC-3 cell extract, and 386.2 ng/mg in THP-1 cell extract samples.

• ELISA

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ELISA - Human Cytochrome C ELISA Kit (AB221832)

Interpolated concentrations of native Cytochrome C in HepG2 cell extract, PC-3 cell extract, and THP-1 cell extract samples based on a 50 μg/mL extract load. The concentrations of Cytochrome C were measured in duplicate and interpolated from the Cytochrome C standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cytochrome C concentration was determined to be 45.64 ng/mL in HepG2 cell extract, 81.23 ng/mL in PC-3 cell extract, and 19.01 ng/mL in THP-1 cell extract.

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ELISA - Human Cytochrome C ELISA Kit (AB221832)

Comparison Cytochrome C distribution in subcellular fractions derived from 3.7x103 HeLa cells and whole cells cultured in the presence (treated) or absence (untreated) of 1 μM staurosporine for 4 hours. Cells were collected directly after treatment and subcellular fractions were prepared using a cell fractionation kit (ab109719). Fractions were processed as described in section 11.10. and assayed. The concentrations of Cytochrome C were measured in three different dilutions of the fraction samples in duplicates and interpolated from the Cytochrome C standard curve. The interpolated values are plotted (mean +/- SD, n=3). The mean Cytochrome C concentration was determined be 171.4 ng/mL in the treated cytosol fraction, 242.8 in the treated mitochondrial fraction, 562.0 in the treated whole cell sample, 407.2 ng/mL in the untreated mitochondrial fraction, and 558.9 ng/mL in the untreated whole cell sample. Cytochrome C was not detectable in the untreated cytosol fraction and in both nuclear fractions.

• ELISA

Supplier Data

ELISA - Human Cytochrome C ELISA Kit (AB221832)

Interpolated concentrations of native Cytochrome C in human heart tissue extract based on a 20 μg/mL extract load, colon tissue extract based on a 50 μg/mL extract load, and skeletal muscle tissue extract based on a 25 μg/mL extract load. The concentrations of Cytochrome C were measured in duplicate and interpolated from the Cytochrome C standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cytochrome C concentration was determined to be 35.41 ng/mL in heart tissue extract, 32.74 ng/mL in colon tissue extract, and 43.12 ng/mL in skeletal muscle tissue extract.