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AB277460

Human Cytoglobin ELISA

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Human Cytoglobin ELISA is a Sandwich (quantitative) ELISA for the measurement of Human Cytoglobin ELISA in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

STAP, CYGB, Cytoglobin, Histoglobin, Nitric oxygen dioxygenase CYGB, Nitrite reductase CYGB, Pseudoperoxidase CYGB, Stellate cell activation-associated protein, Superoxide dismutase CYGB, HGb, NOD

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ELISA - Human Cytoglobin ELISA (AB277460)
  • ELISA

Supplier Data

ELISA - Human Cytoglobin ELISA (AB277460)

These standard curves are for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.3 ng/mL

Range

0.287 - 70 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Product details

Human Cytoglobin ELISA Kit (ab277460) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Cytoglobin in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Human Cytoglobin coated on a 96-well plate. Standards and samples are pipetted into the wells and Human Cytoglobin present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human Cytoglobin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human Cytoglobin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Serum", "range": "82 - 101 %", "average": "= 90.59" }, { "sample": "Plasma", "range": "84 - 94 %", "average": "= 87.98" }, { "sample": "Cell culture media", "range": "69 - 87 %", "average": "= 74.86" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab277460-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB277460-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB277460-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human Cytoglobin coated Microplate", "number":"AB277460-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human Cytoglobin Antibody", "number":"AB277460-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB277460-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB277460-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Cytoglobin standard protein (Lyophilized)", "number":"AB277460-CMP07", "productcode":"" }, { "size":"1 x 200 µL", "name":"600X HRP-Streptavidin Concentrate", "number":"AB277460-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytoglobin also known as CYGB is a globin protein with a molecular weight of approximately 21 kDa. This protein is part of the vertebrate globin family and exhibits oxygen-binding properties. Cytoglobin is mainly expressed in fibroblasts and is detectable in various tissues including the liver brain and kidney. It functions as an intracellular hemoprotein which allows it to facilitate oxygen transport and storage similar to other globins like hemoglobin and myoglobin.
Biological function summary

This globin plays a role in cellular protective mechanisms. Cytoglobin participates in protecting cells from hypoxic and oxidative stress thereby contributing to oxygen homeostasis. Although it is not part of a known protein complex its ability to bind oxygen and reactive oxygen species indicates its significant role in mitigating cellular damage. By maintaining a balance of oxygen levels inside cells cytoglobin helps preserve cellular integrity under stressful conditions.

Pathways

Cytoglobin interacts in significant biological processes related to oxygen regulation. It is closely involved in the hypoxia response pathway where it aids in adapting cells to low oxygen environments. Cytoglobin operates alongside proteins such as hypoxia-inducible factors (HIFs) which help regulate gene expression in response to oxygen availability. It also plays a role in the oxidative stress response connecting to systems involving antioxidant proteins that help mitigate the effects of reactive oxygen species.

Cytoglobin has links to conditions like cancer and fibrotic diseases. Its role in oxygen regulation and response to hypoxia makes it relevant in tumor biology as tumors often create hypoxic environments that promote cancer progression. Cytoglobin's connection to cancer involves interactions with hypoxia-inducible factors that drive tumor adaptability. Additionally in fibrotic diseases such as liver fibrosis cytoglobin's expression levels correlate with disease severity suggesting it as a marker in fibrosis progression. This relation implies potential interactions with proteins like transforming growth factor-beta (TGF-β) which plays a role in fibrotic tissue development.
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Product protocols

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Target data

Probable multifunctional globin with a hexacoordinated heme iron required for the catalysis of various reactions depending on redox condition of the cell as well as oxygen availability (PubMed : 11893755, PubMed : 12359339, PubMed : 15165856, PubMed : 19147491, PubMed : 20511233, PubMed : 28393874, PubMed : 28671819, PubMed : 29128400, PubMed : 33576020, PubMed : 34930834). Has a nitric oxide dioxygenase (NOD) activity and is most probably involved in cell-mediated and oxygen-dependent nitric oxide consumption (PubMed : 19147491, PubMed : 20511233, PubMed : 28393874, PubMed : 28671819). By scavenging this second messenger may regulate several biological processes including endothelium-mediated vasodilation and vascular tone (PubMed : 19147491, PubMed : 28393874). Under normoxic conditions functions as a nitric oxide dioxygenase (NOD) but under hypoxic conditions the globin may switch its function to that of a nitrite (NO2) reductase (NiR), generating nitric oxide (PubMed : 29128400). Could also have peroxidase and superoxide dismutase activities, detoxifying reactive oxygen species and protecting cells against oxidative stress (PubMed : 12359339, PubMed : 33576020, PubMed : 34930834). Also binds dioxygen with low affinity and could function as an oxygen sensor but has probably no function as a respiratory oxygen carrier (PubMed : 11893755, PubMed : 15299006, PubMed : 20553503).
See full target information CYGB
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