AB226895

Human Cytokeratin 14 ELISA Kit

Recombinant
SimpleStep
What is this?

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(3 Publications)

Human Cytokeratin 14 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Cytokeratin 14 with a sensitivity of 4.9 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Cytokeratin-14, Keratin-14, CK-14, K14, KRT14

7 Images

Sandwich ELISA - Human Cytokeratin 14 ELISA Kit (AB226895)

sELISA

Supplier Data

Sandwich ELISA - Human Cytokeratin 14 ELISA Kit (AB226895)

Representative image of Human Cytokeratin 14 ELISA Kit (ab226895)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Human Cytokeratin 14 ELISA Kit Capture Antibody

- 10X Human Cytokeratin 14 Detector Antibody

- 10X Human Cytokeratin 14 ELISA Kit Lyophilised Purified Protein (2 vials)

- Human Cytokeratin 14 ELISA Kit 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody Diluent 4BR

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

sELISA

Supplier Data

Sandwich ELISA - Human Cytokeratin 14 ELISA Kit (AB226895)

Example of Human Cytokeratin 14 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Cytokeratin 14 ELISA Kit (AB226895)

Interpolated concentrations of native Cytokeratin 14 in Human skin extract and HACAT cell extract.

The concentrations of Cytokeratin 14 were measured in duplicate and interpolated from the Cytokeratin 14 standard curve and corrected for sample dilution and extract load. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cytokeratin 14 concentration was determined to be 396 pg/μg skin extract and 491 pg/μg in HACAT cell extract.

sELISA

Supplier Data

Sandwich ELISA - Human Cytokeratin 14 ELISA Kit (AB226895)

Interpolated concentrations of native Cytokeratin 14 in Human saliva and urine samples.

The concentrations of Cytokeratin 14 were measured in duplicates, interpolated from the Cytokeratin 14 standard curves and corrected for sample dilution. Undiluted samples are as follows : saliva 50% and urine 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cytokeratin 14 concentration was determined to be 3.0 ng/mL in saliva and 1.06 ng/mL in urine.

sELISA

Supplier Data

Sandwich ELISA - Human Cytokeratin 14 ELISA Kit (AB226895)

Example of Human Cytokeratin 14 standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Human Cytokeratin 14 ELISA Kit (AB226895)

Example of human Cytokeratin 14 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Human Cytokeratin 14 ELISA Kit (AB226895)

Interpolated concentration of native Cytokeratin 14 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are HACAT extract 3.75 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Key facts

Detection method

Colorimetric

Sample types

Saliva, Urine, Cell culture extracts, Tissue Extracts

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 4.9 pg/mL

Range

31.3 - 2000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Cytokeratin 14 ELISA Kit (ab226895) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Cytokeratin 14 protein in saliva, tissue extracts, urine, and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Cytokeratin 14 with 4.9 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Cytokeratin 14 is an intermediate filament protein in the keratin family and is a component of the cytoskeletal scaffold in epithelial cells. Cytokeratin 14 is mutated in epidermolysis bullosa simplex, Naegeli-Franceschetti-Jadassohn syndrome and Dermatopathia pigmentosa reticularis. Cytokeratin 14 is a 472-residue protein and the antibodies in this kit were raised to the C-terminal 357 residues.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Urine", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "11.1" }, { "reproducibilityType": "Intra", "sample": "Urine", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.1" } ]

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Recovery

[ { "sample": "Saliva", "range": "99 - 106 %", "average": "= 102" }, { "sample": "Urine", "range": "108 - 114 %", "average": "= 110" }, { "sample": "Cell culture extracts", "range": "93 - 106 %", "average": "= 99" }, { "sample": "Tissue Extracts", "range": "88 - 94 %", "average": "= 91" } ]

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What's included?

{ "values": { "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab226895-1x384Tests", "assetComponentDetails": [ { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB226895-CMP04" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB226895-CMP12" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB226895-CMP01" }, { "size":"1 x 600 µL", "name":"10X Human Cytokeratin 14 Detector Antibody", "number":"AB226895-CMP09" }, { "size":"2 x 1 Vial", "name":"Human Cytokeratin 14 Lyophilized Recombinant Protein", "number":"AB226895-CMP10" }, { "size":"1 x 50 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB226895-CMP07" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB226895-CMP11" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB226895-CMP05" }, { "size":"1 x 600 µL", "name":"10X Human Cytokeratin 14 Capture Antibody", "number":"AB226895-CMP08" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB226895-CMP14" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab226895-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB226895-CMP04", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB226895-CMP01", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB226895-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB226895-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB226895-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB226895-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB226895-CMP11", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Cytokeratin 14 Lyophilized Recombinant Protein", "number":"AB226895-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Cytokeratin 14 Capture Antibody", "number":"AB226895-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Cytokeratin 14 Detector Antibody", "number":"AB226895-CMP09", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB226895-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB226895-CMP03", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytokeratin 14 also known as CK14 keratin 14 or KRT14 is a type I keratin protein with a molecular mass of approximately 50 kDa. It forms intermediate filaments in epithelial cells primarily in the basal layer of stratified epithelium and is part of the cytoskeleton. Cytokeratin 14 expression appears in tissues such as the epidermis tongue and esophagus where it contributes to the structural integrity and mechanical stability of cells. Laboratories often utilize antibodies like anti-K14 and labels such as Alexa Fluor 555 or Alexa Fluor 647 to identify cytokeratin 14 in research settings.

Biological function summary

This protein significantly contributes to the maintenance and regeneration of epithelial tissues by forming a network of filaments with keratin 5. KRT14 pairs with keratin 5 to create a keratin intermediate filament complex which plays a structural role in the resilience and elasticity of epithelial tissues. This partnership is essential for the assembly of the cytoskeleton and supports the protective barrier function of the skin and related tissues.

Pathways

Cytokeratin 14 engages in pivotal cellular processes like keratinization and wound healing. The protein is actively involved in signal transduction pathways such as the epithelial cell differentiation pathway. Within these pathways KRT14 collaborates with KRT5 and other structural proteins to regulate cell proliferation and differentiation reflecting its importance in skin health and function.

Cytokeratin 14 associates with specific genetic conditions including epidermolysis bullosa simplex (EBS) and squamous cell carcinoma (SCC). In EBS mutations in the KRT14 gene lead to skin fragility. In SCC altered expression of KRT14 and related proteins such as keratin 5 can influence tumor progression and invasion. These associations highlight the critical role of KRT14 in both normal and pathological epithelial function.

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Product protocols

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Target data

The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro.

See full target information Keratin, type I cytoskeletal 14

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Publications (3)

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Cytokeratin 14 ELISA Kit

Sandwich ELISA - Human Cytokeratin 14 ELISA Kit (AB226895)

Sandwich ELISA - Human Cytokeratin 14 ELISA Kit (AB226895)

Sandwich ELISA - Human Cytokeratin 14 ELISA Kit (AB226895)

Sandwich ELISA - Human Cytokeratin 14 ELISA Kit (AB226895)

Sandwich ELISA - Human Cytokeratin 14 ELISA Kit (AB226895)

Sandwich ELISA - Human Cytokeratin 14 ELISA Kit (AB226895)

Sandwich ELISA - Human Cytokeratin 14 ELISA Kit (AB226895)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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Primary Antibodies

Primary Antibodies

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