Human Cytokeratin 18 ELISA Kit (ab227896)

Human Cytokeratin 18 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Cytokeratin 18 with a sensitivity of 3.4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

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ELISA - Human Cytokeratin 18 ELISA Kit (AB227896), expandable thumbnail

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Key facts

Detection method: Colorimetric
Sample types: Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
Assay type: Sandwich (quantitative)
Reactive species: Human
Range: 15.6 - 1000 pg/mL
Assay time: 1h 30m
Sensitivity: = 3.4 pg/mL

Reactivity data

Application	Reactivity	Dilution info	Notes
Application sELISA	Reactivity Reacts	Dilution info -	Notes -

Target data

See full target information KRT18

Function

Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

Alternative names

CYK18, PIG46, KRT18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, Keratin-18, CK-18, K18

What's included?

1 x 96 Tests

Components

10X Human Cytokeratin 18 Capture Antibody

1 x 600 µL

10X Human Cytokeratin 18 Detector Antibody

1 x 600 µL

10X Wash Buffer PT (ab206977)

1 x 20 mL

50X Cell Extraction Enhancer Solution (ab193971)

1 x 1 mL

5X Cell Extraction Buffer PTR (ab193970)

1 x 10 mL

Antibody Diluent 4BI

1 x 6 mL

Human Cytokeratin 18 Lyophilized Recombinant Protein

2 x 1 Vial

Plate Seals

1 x 1 Unit

Sample Diluent LS

1 x 50 mL

SimpleStep Pre-Coated 96-Well Microplate (ab206978)

1 x 1 Unit

Stop Solution

1 x 12 mL

TMB Development Solution

1 x 12 mL

Key facts

Detection method: Colorimetric
Sample types: Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
Assay type: Sandwich (quantitative)
Reactive species: Human
Range: 15.6 - 1000 pg/mL
Assay time: 1h 30m
Assay Platform: Pre-coated microplate (12 x 8 well strips)
Sensitivity: = 3.4 pg/mL

Precision

Intra assay

Sample	n	C.V.
Sample Overall	n 5	C.V. 2.1

Inter assay

Sample	n	C.V.
Sample Overall	n 3	C.V. 8.4

Recovery

Sample specific recovery

Sample type	Average %	Range
Sample type Serum	Average % = 100	Range 99 - 102 %
Sample type EDTA Plasma	Average % = 99	Range 97 - 100 %
Sample type Cell culture extracts	Average % = 110	Range 106 - 115 %
Sample type Heparin Plasma	Average % = 99	Range 97 - 99 %
Sample type Citrate plasma	Average % = 95	Range 93 - 97 %
Sample type Cell culture media	Average % = 109	Range 107 - 110 %

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Storage information: +4°C

Notes

Human Cytokeratin 18 ELISA Kit ab227896 is a rapid single-wash 90-min Sandwich ELISA to measure Human Cytokeratin 18 in cell culture extracts, cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum, tissue extracts. This SimpleStep sensitivity is 3.4 pg/mL.

How the assay works

Human Cytokeratin 18 SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Cytokeratin 18 ELISA Kit ab227896 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Cytokeratin 18 (CK18) also known as Keratin 18 or KRT18 is a type I intermediate filament protein with a molecular weight of approximately 45 kDa. It plays a mechanical role in stabilizing epithelial cell structure and function. CK18 is expressed in single-layered epithelial tissues such as the liver intestine and pancreas. It partners with the type II keratin usually KRT8 to form a stable cytoskeletal network in these cells providing structural integrity and resilience.

Biological function summary

CK18 maintains cellular integrity and mediates resistance to mechanical and non-mechanical stresses in epithelial cells. It forms heterodimers with KRT8 participating in a complex network of filaments important for maintaining cell shape and stability. This organization plays a role in managing cell survival during stress and apoptosis helping regulate cellular functions and responses in tissues where it is expressed.

Pathways

Cytokeratin 18 is involved in the apoptosis and cellular stress response pathways. It interacts with proteins such as caspases during apoptosis facilitating cytoskeletal reorganization and cell fragmentation. CK18 in coordination with KRT8 also participates in signaling pathways that regulate cell cycle and apoptotic processes underlining its importance in cellular turnover and tissue homeostasis.

Associated diseases and disorders

Elevated levels of CK18 fragments are often observed in cases of non-alcoholic steatohepatitis (NASH) and breast cancer. It serves as a biomarker for liver injury due to its abundance in hepatocytes and release upon cell death. In breast cancer alterations in CK18 expression can reflect disease progression where it interacts with other proteins such as vimentin indicating epithelial-to-mesenchymal transition a process that facilitates metastasis.

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8 product images

ELISA - Human Cytokeratin 18 ELISA Kit (ab227896)
Sandwich ELISA - Human Cytokeratin 18 ELISA Kit (ab227896)
Example of Human Cytokeratin 18 standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Cytokeratin 18 ELISA Kit (ab227896)
Example of Human Cytokeratin 18 standard curve in 1X Cell Extraction Buffer PTR.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Cytokeratin 18 ELISA Kit (ab227896)
Interpolated concentrations of native Cytokeratin 18 in Human serum and plasma samples.
The concentrations of Cytokeratin 18 were measured in duplicates, interpolated from the Cytokeratin 18 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (EDTA) 50%, plasma (heparin) 50% and plasma (citrate) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cytokeratin 18 concentration was determined to be 23 pg/mL in serum, 29pg/mL in plasma (EDTA), 96 pg/mL in plasma (heparin), and 33 pg/mL in plasma (citrate).
Sandwich ELISA - Human Cytokeratin 18 ELISA Kit (ab227896)
Serum from ten individual healthy Human female donors were measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). 5/10 donors were below the detectable dose. The mean of the detectable donors Cytokeratin 18 concentration was determined to be 59 pg/mL with a range of 31– 79 pg/mL.
Sandwich ELISA - Human Cytokeratin 18 ELISA Kit (ab227896)
Interpolated concentrations of spiked Cytokeratin 18 in Human serum and plasma samples.
The concentrations of Cytokeratin 18 were measured in duplicates, interpolated from the Cytokeratin 18 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (EDTA) 50%, plasma (heparin) 50% and plasma (citrate) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Sandwich ELISA - Human Cytokeratin 18 ELISA Kit (ab227896)
Interpolated concentrations of native Cytokeratin 18 in Human cell culture supernatant sample.
The concentrations of Cytokeratin 18 were measured in duplicates, interpolated from the Cytokeratin 18 standard curves and corrected for sample dilution. Undiluted samples are as follows: Hela cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cytokeratin 18 concentration was determined to be 1000 pg/mL in Hela cell culture supernatant.
Sandwich ELISA - Human Cytokeratin 18 ELISA Kit (ab227896)
Interpolated concentrations of native Cytokeratin 18 in Hela cell extract and Human Liver tissue extract.
The concentrations of Cytokeratin 18 were measured in duplicate and interpolated from the Cytokeratin 18 standard curve and corrected for sample dilution and extract load. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cytokeratin 18 concentration was determined to be 0.89 pg / μg Hela extract and 35 pg/ μg Liver extract.