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AB267810

Human Cytokeratin 8 ELISA Kit

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Human Cytokeratin 8 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Cytokeratin 8 with a sensitivity of 1.01 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CYK8, KRT8, Cytokeratin-8, Keratin-8, Type-II keratin Kb8, CK-8, K8

2 Images
Sandwich ELISA - Human Cytokeratin 8 ELISA Kit (AB267810)
  • sELISA

Supplier Data

Sandwich ELISA - Human Cytokeratin 8 ELISA Kit (AB267810)

Example of human Cytokeratin 8 standard curve in 1X Cell Extraction Buffer PTR.

The Cytokeratin 8 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Cytokeratin 8 ELISA Kit (AB267810)
  • sELISA

Supplier Data

Sandwich ELISA - Human Cytokeratin 8 ELISA Kit (AB267810)

Interpolated concentrations of native Cytokeratin 8 in human MCF7, HepG2, and liver homogenate extract samples based on 6.25 μg/mL, 31.25 μg/mL, and 100 μg/mL extract loads, respectively.

The concentrations of Cytokeratin 8 were measured in duplicate and interpolated from the Cytokeratin 8 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cytokeratin 8 concentration was determined to be 26.10 ng/mL in MCF7 extract, 10.03 ng/ml in HepG2 extract, and 6.71 ng/ml in liver homogenate extract.

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts, Cell Lysate

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 1.01 ng/mL

Range

1.25 - 80 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Cytokeratin 8 ELISA Kit (ab267810) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Cytokeratin 8 protein in cell culture media, tissue extracts, and cell lysate. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Cytokeratin 8 with 1.01 ng/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Keratin, type II cytoskeletal 8 is a major component of the intermediate filament cytoskeleton and is a marker for epithelial cells. Abnormal expression of Cytokeratin 8 is found in various forms of cancer, and Cytokeratin 8 has been used as a marker for circulating tumor cells.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.7" } ]
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Recovery

[ { "sample": "Tissue Extracts", "range": null, "average": "= 102" }, { "sample": "Cell Lysate", "range": null, "average": "= 101" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab267810-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB267810-CMP06", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB267810-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB267810-CMP12", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB267810-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Cytokeratin 8 Capture Antibody", "number":"AB267810-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Cytokeratin 8 Lyophilized Recombinant Protein", "number":"AB267810-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB267810-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Cytokeratin 8 Detector Antibody", "number":"AB267810-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB267810-CMP10", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB267810-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB267810-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
See full target information KRT8
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websiteProtocolBooklet
en
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com