Human Anti-Cytomegalovirus IgG ELISA Kit (CMV)

Product details

Human Anti-Cytomegalovirus IgG ELISA Kit (CMV) is an indirect ELISA kit for the qualitative detection of human IgG antibodies against CMV with a 98% sensitivity.

How the assay works

Human Anti-Cytomegalovirus IgG ELISA Kit (CMV)ab108724 is an indirect ELISA kit.
A 96-well plate has been precoated with Cytomegalovirus antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Cytomegalovirus-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Cytomegalovirus IgG sample captured in plate.

Assay specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility.

Human Anti-Cytomegalovirus IgG ELISA Kit (CMV)ab108724 protocol summary

1. Add controls and samples to appropriate wells - Incubate for 1hr
2. Wash wells
3. add anti-cytomegalovirus IgG-HRP conjugate - Incubate for 30 min
4. Wash
5. add TMB substrate - Incubate for 15 min
6. Add Stop solution. Read at 450 nm within 30 min.

How other researchers are using Human Anti-Cytomegalovirus IgG ELISA Kit (CMV) ab108724

Human Anti-Cytomegalovirus IgG ELISA Kit (CMV)ab108724 has been used to assess the effect of CMV seropositivity on ChAdOx1 vaccination immunogenicity ⁽¹⁾ and to study immunoageing in T-cell compartments ⁽²⁾.

References
⁽¹⁾ HR Sharpe et.al. PMID 35192547
⁽²⁾ Ni Zeng et al. PMID 35037711

Related and Recommended products

Other ELISA kits can be used to assess CMV serostatus together with Human Anti-Cytomegalovirus IgG ELISA Kit (CMV)ab108724:
- Human Anti-Cytomegalovirus IgM ELISA Kit (CMV) ab108725

Epstein-Barr virus (EBV) is another common herpesvirus causing similar symptoms. ELISA kits to assess EBV serostatus are:
- Human Anti-Epstein Barr virus IgG ELISA Kit (EBV-VCA) ab108730
- Human Anti-Epstein Barr virus IgG ELISA Kit (EBV-EBNA) ab108731

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.