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AB315311

Human D-Dimer, Fluorescent ELISA Kit

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Human D-Dimer, Fluorescent ELISA Kit is a single-wash 90-min Simplestep used to quantify Human D-Dimer with a sensitivity of 0.065 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Fluorescent Sandwich ELISA - 530/570/590 nm readout : works on any standard plate reader
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Sandwich ELISA - Human D-Dimer, Fluorescent ELISA Kit (AB315311)
  • sELISA

Supplier Data

Sandwich ELISA - Human D-Dimer, Fluorescent ELISA Kit (AB315311)

Example of Human D-Dimer standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human D-Dimer, Fluorescent ELISA Kit (AB315311)
  • sELISA

Supplier Data

Sandwich ELISA - Human D-Dimer, Fluorescent ELISA Kit (AB315311)

Interpolated concentration of native D-Dimer was measured in duplicate at different sample concentrations. Undiluted samples are as follows : Human serum 1 : 200, plasma (citrate) 1 : 1,250, plasma (EDTA) 1 : 25, and plasma (heparin) 1 : 25.  The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human D-Dimer, Fluorescent ELISA Kit (AB315311)
  • sELISA

Supplier Data

Sandwich ELISA - Human D-Dimer, Fluorescent ELISA Kit (AB315311)

Serum of eight individual healthy Human Female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean D-Dimer concentration was determined to be 657 ng/mL with a range of 68 – 1,356 ng/mL.

Key facts

Detection method

Fluorescent

Sample types

Serum, EDTA Plasma, Citrate plasma, Heparin Plasma, Urine, Cell culture media

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.065 ng/mL

Range

0.2 - 100 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human D-Dimer, Fluorescent ELISA kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of D-Dimer, Fluorescent protein in Serum, EDTA Plasma, Cit Plasma, Hep Plasma, Urine, and Cell culture media. Quantitate Human D-Dimer, Fluorescent with 0.065 ng/ml sensitivity.

This CatchPoint SimpleStep ELISA kit has been optimized for Molecular Devices Microplate Readers. Click here for a list of recommended Microplate Readers.
If using a Molecular Devices’ plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint SimpleStep ELISA Kits is available with all the protocol and analysis settings at www.softmaxpro.org.

The CatchPoint SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material.  CatchPoint HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission.

D-dimer is a protein formed by the cross-linking of two D fragments of the fibrin protein. D-dimer is one of several fibrin degradation products (FDPs) formed by the degradation of a blood clot by fibrinolysis. Its measurement is used to diagnose the blood disorder disseminated intravascular coagulation and in the diagnosis of thrombosis.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 7.4" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.8" } ]
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Recovery

[ { "sample": "Serum", "range": "92 - 98 %", "average": "= 95" }, { "sample": "EDTA Plasma", "range": "101 - 113 %", "average": "= 105" }, { "sample": "Citrate plasma", "range": "99 - 113 %", "average": "= 106" }, { "sample": "Heparin Plasma", "range": "97 - 104 %", "average": "= 101" }, { "sample": "Urine", "range": "85 - 93 %", "average": "= 90" }, { "sample": "Cell culture media", "range": "86 - 99 %", "average": "= 92" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

See full target information D-Dimer
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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