Human D-Dimer, Fluorescent ELISA Kit is a single-wash 90-min Simplestep used to quantify Human D-Dimer with a sensitivity of 0.065 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Fluorescent Sandwich ELISA - 530/570/590 nm readout : works on any standard plate reader
Images
Key facts
- Detection method
- Fluorescent
- Sample types
- Serum, EDTA Plasma, Citrate plasma, Heparin Plasma, Urine, Cell culture media
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.2 - 100 ng/mL
- Assay time
- 1h 30m
- Sensitivity
- = 0.065 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
What's included?
Human D-Dimer, Fluorescent ELISA Kit is a single-wash 90-min Simplestep used to quantify Human D-Dimer with a sensitivity of 0.065 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Fluorescent Sandwich ELISA - 530/570/590 nm readout : works on any standard plate reader
Key facts
- Detection method
- Fluorescent
- Sample types
- Serum, EDTA Plasma, Citrate plasma, Heparin Plasma, Urine, Cell culture media
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.2 - 100 ng/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 0.065 ng/mL
- General recovery
- 95%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. = 7.4 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. = 3.8 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 95 | Range 92 - 98 % |
Sample type EDTA Plasma | Average % = 105 | Range 101 - 113 % |
Sample type Citrate plasma | Average % = 106 | Range 99 - 113 % |
Sample type Heparin Plasma | Average % = 101 | Range 97 - 104 % |
Sample type Urine | Average % = 90 | Range 85 - 93 % |
Sample type Cell culture media | Average % = 92 | Range 86 - 99 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human D-Dimer, Fluorescent ELISA kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of D-Dimer, Fluorescent protein in Serum, EDTA Plasma, Cit Plasma, Hep Plasma, Urine, and Cell culture media. Quantitate Human D-Dimer, Fluorescent with 0.065 ng/ml sensitivity.
This CatchPoint SimpleStep ELISA kit has been optimized for Molecular Devices Microplate Readers. Click here for a list of recommended Microplate Readers.
If using a Molecular Devices’ plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint SimpleStep ELISA Kits is available with all the protocol and analysis settings at www.softmaxpro.org.
The CatchPoint SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPoint HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission.
D-dimer is a protein formed by the cross-linking of two D fragments of the fibrin protein. D-dimer is one of several fibrin degradation products (FDPs) formed by the degradation of a blood clot by fibrinolysis. Its measurement is used to diagnose the blood disorder disseminated intravascular coagulation and in the diagnosis of thrombosis.
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3 product images
Sandwich ELISA - Human D-Dimer, Fluorescent ELISA Kit (ab315311)
Example of Human D-Dimer standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human D-Dimer, Fluorescent ELISA Kit (ab315311)
Interpolated concentration of native D-Dimer was measured in duplicate at different sample concentrations. Undiluted samples are as follows: Human serum 1:200, plasma (citrate) 1:1,250, plasma (EDTA) 1:25, and plasma (heparin) 1:25. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human D-Dimer, Fluorescent ELISA Kit (ab315311)
Serum of eight individual healthy Human Female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean D-Dimer concentration was determined to be 657 ng/mL with a range of 68 – 1,356 ng/mL.
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