AB256405

Human DAF ELISA Kit (CD55)

Recombinant
SimpleStep
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(1 Publication)

Human DAF ELISA Kit (CD55) is a single-wash 90-min Simplestep used to quantify Human DAF (CD55) with a sensitivity of 14.27 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD55, CR, DAF, Complement decay-accelerating factor

5 Images

Sandwich ELISA - Human DAF ELISA Kit (CD55) (AB256405)

sELISA

Supplier Data

Sandwich ELISA - Human DAF ELISA Kit (CD55) (AB256405)

Interpolated concentrations of native DAF in human serum and plasma samples.

The concentrations of DAF were measured in duplicates, interpolated from the DAF standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 2.5%, plasma (citrate) 2.5%, plasma (EDTA) 2.5% and plasma (heparin) 2.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean DAF concentration was determined to be 58.92 ng/mL in serum, 42.96 ng/mL in plasma (citrate), 68.42 ng/mL in plasma (EDTA), and 50.21 ng/mL in plasma (heparin).

sELISA

Supplier Data

Sandwich ELISA - Human DAF ELISA Kit (CD55) (AB256405)

Interpolated concentrations of native DAF in human lung extract based on a 100 μg/mL extract load and human placenta extract based on a 50 μg/mL extract load.

The concentrations of DAF were measured in duplicate and interpolated from the DAF standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean DAF concentration was determined to be 4104.76 pg/mL in lung extract and 2085.73 pg/mL in placenta extract.

sELISA

Supplier Data

Sandwich ELISA - Human DAF ELISA Kit (CD55) (AB256405)

1.25% Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean DAF concentration was determined to be 48.15 ng/mL with a range of 20.39 – 65.50 ng/mL.

sELISA

Supplier Data

Sandwich ELISA - Human DAF ELISA Kit (CD55) (AB256405)

Example of human DAF standard curve in Sample Diluent NS.

The DAF standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human DAF ELISA Kit (CD55) (AB256405)

Interpolated concentrations of native DAF in OVCAR-3 cell culture supernatant samples.

The concentrations of DAF were measured in duplicates, interpolated from the DAF standard curves and corrected for sample dilution. Undiluted samples are as follows : OVCAR-3 supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean DAF concentration was determined to be 2261.44 pg/mL in OVCAR-3 supernatant.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Heparin Plasma, Citrate plasma, Serum, Cell Lysate, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 14.27 pg/mL

Range

62.5 - 4000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human DAF ELISA Kit (CD55) (ab256405) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of DAF (CD55) protein in cell culture media, cell lysate, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human DAF (CD55) with 14.27 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Decay-Accelerating Factor (DAF), also known as CD55, is a 70kD membrane protein that regulates the on the surface. DAF recognizes C4b and C3b fragments that are created during activation of C4 ( or pathway) or C3 ( pathway). Interaction of DAF with cell-associated C4b of the classical and lectin pathways interferes with the conversion of C2 to C2b, thereby preventing formation of the C4b2b . Interaction of DAF with C3b of the alternative pathway blocks the conversion of factor B to Bb by factor D, thereby preventing formation of the C3bBb C3 convertase of the alternative pathway. By limiting the amplification convertases of the complement cascade, DAF indirectly blocks the formation of the . DAF shares 47%, 45%, and 56% sequence homology with mouse, rat, and cow respectively.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.3" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.7" } ]

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Recovery

[ { "sample": "Serum", "range": "80 - 90 %", "average": "= 86" }, { "sample": "EDTA Plasma", "range": "96 - 99 %", "average": "= 97" }, { "sample": "Cell culture media", "range": "94 - 114 %", "average": "= 105" }, { "sample": "Heparin Plasma", "range": "101 - 103 %", "average": "= 102" }, { "sample": "Citrate plasma", "range": "82 - 88 %", "average": "= 85" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab256405-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB256405-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB256405-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB256405-CMP07", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB256405-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB256405-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB256405-CMP11", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB256405-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human DAF (CD55) Lyophilized Recombinant Protein", "number":"AB256405-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human DAF (CD55) Capture Antibody", "number":"AB256405-CMP01", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB256405-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human DAF (CD55) Detector Antibody", "number":"AB256405-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB256405-CMP04", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD55 also known as Decay-Accelerating Factor (DAF) is a glycoprotein that regulates the complement cascade. This protein typically weighs around 70 kDa. It is expressed in many cells including epithelial and endothelial cells and is often present on the surface of blood cells. CD55 plays an important role in protecting cells against lysis by inhibiting the formation and stability of C3 and C5 convertases which ultimately prevent cell destruction by the complement system.

Biological function summary

CD55 helps maintain immune homeostasis by preventing the complement-mediated damage to host tissues. It functions as a member of the regulators of complement activation (RCA) family. While not necessarily part of a larger protein complex its function is related to other regulatory proteins such as CD46 and CD59 which cooperate to regulate complement activity. The DAF protein's immunology function is instrumental in ensuring that self-cells are not inadvertently attacked by the body's immune system.

Pathways

CD55 significantly impacts the complement pathway which is a central component of the innate immune system. It directly interacts with complement components C3 and C5 accelerating their decay and disassembly. Through this pathway CD55 has a working relationship with other complement regulatory proteins like Factor H which also aids in controlling complement activation on the cell surface.

A dysfunctional CD55 can relate to conditions such as paroxysmal nocturnal hemoglobinuria (PNH) and atypical hemolytic uremic syndrome (aHUS). In PNH the absence or insufficiency of CD55 (and CD59) on red blood cells leads to complement-mediated lysis causing hemolytic anemia. In aHUS impaired regulation of the complement system including abnormalities in CD55 function exacerbates symptoms. CD55's role is therefore important in preventing these complement-related disorders and preserving cellular integrity in the human body.

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Product protocols

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Target data

This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade (PubMed : 7525274). Inhibits complement activation by destabilizing and preventing the formation of C3 and C5 convertases, which prevents complement damage (PubMed : 28657829).. (Microbial infection) Acts as a receptor for Coxsackievirus A21, coxsackieviruses B1, B3 and B5.. (Microbial infection) Acts as a receptor for Human enterovirus 70 and D68 (Probable).. (Microbial infection) Acts as a receptor for Human echoviruses 6, 7, 11, 12, 20 and 21.

See full target information CD55

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell 182:1044-1061.e18 PubMed32795414

2020

Extracellular Vesicle and Particle Biomarkers Define Multiple Human Cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Ayuko Hoshino,Han Sang Kim,Linda Bojmar,Kofi Ennu Gyan,Michele Cioffi,Jonathan Hernandez,Constantinos P Zambirinis,Gonçalo Rodrigues,Henrik Molina,Søren Heissel,Milica Tesic Mark,Loïc Steiner,Alberto Benito-Martin,Serena Lucotti,Angela Di Giannatale,Katharine Offer,Miho Nakajima,Caitlin Williams,Laura Nogués,Fanny A Pelissier Vatter,Ayako Hashimoto,Alexander E Davies,Daniela Freitas,Candia M Kenific,Yonathan Ararso,Weston Buehring,Pernille Lauritzen,Yusuke Ogitani,Kei Sugiura,Naoko Takahashi,Maša Alečković,Kayleen A Bailey,Joshua S Jolissant,Huajuan Wang,Ashton Harris,L Miles Schaeffer,Guillermo García-Santos,Zoe Posner,Vinod P Balachandran,Yasmin Khakoo,G Praveen Raju,Avigdor Scherz,Irit Sagi,Ruth Scherz-Shouval,Yosef Yarden,Moshe Oren,Mahathi Malladi,Mary Petriccione,Kevin C De Braganca,Maria Donzelli,Cheryl Fischer,Stephanie Vitolano,Geraldine P Wright,Lee Ganshaw,Mariel Marrano,Amina Ahmed,Joe DeStefano,Enrico Danzer,Michael H A Roehrl,Norman J Lacayo,Theresa C Vincent,Martin R Weiser,Mary S Brady,Paul A Meyers,Leonard H Wexler,Srikanth R Ambati,Alexander J Chou,Emily K Slotkin,Shakeel Modak,Stephen S Roberts,Ellen M Basu,Daniel Diolaiti,Benjamin A Krantz,Fatima Cardoso,Amber L Simpson,Michael Berger,Charles M Rudin,Diane M Simeone,Maneesh Jain,Cyrus M Ghajar,Surinder K Batra,Ben Z Stanger,Jack Bui,Kristy A Brown,Vinagolu K Rajasekhar,John H Healey,Maria de Sousa,Kim Kramer,Sujit Sheth,Jeanine Baisch,Virginia Pascual,Todd E Heaton,Michael P La Quaglia,David J Pisapia,Robert Schwartz,Haiying Zhang,Yuan Liu,Arti Shukla,Laurence Blavier,Yves A DeClerck,Mark LaBarge,Mina J Bissell,Thomas C Caffrey,Paul M Grandgenett,Michael A Hollingsworth,Jacqueline Bromberg,Bruno Costa-Silva,Hector Peinado,Yibin Kang,Benjamin A Garcia,Eileen M O'Reilly,David Kelsen,Tanya M Trippett,David R Jones,Irina R Matei,William R Jarnagin,David Lyden

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Human DAF ELISA Kit (CD55)

Sandwich ELISA - Human DAF ELISA Kit (CD55) (AB256405)

Sandwich ELISA - Human DAF ELISA Kit (CD55) (AB256405)

Sandwich ELISA - Human DAF ELISA Kit (CD55) (AB256405)

Sandwich ELISA - Human DAF ELISA Kit (CD55) (AB256405)

Sandwich ELISA - Human DAF ELISA Kit (CD55) (AB256405)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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Product promise