AB317544

Human DAP12 ELISA Kit

SimpleStep
Recombinant
What is this?

Be the first to review this product! Submit a review

(0 Publication)

Human DAP12 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human DAP12 with a sensitivity of 2 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

DAP12, KARAP, TYROBP, TYRO protein tyrosine kinase-binding protein, DNAX-activation protein 12, Killer-activating receptor-associated protein, KAR-associated protein

4 Images

Sandwich ELISA - Human DAP12 ELISA Kit (AB317544)

sELISA

Supplier Data

Sandwich ELISA - Human DAP12 ELISA Kit (AB317544)

Interpolated concentration of native DAP12 was measured in duplicate at different sample concentrations. Undiluted samples are 60 µg/mL THP-1 cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

sELISA

Supplier Data

Sandwich ELISA - Human DAP12 ELISA Kit (AB317544)

Example of human DAP12 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human DAP12 ELISA Kit (AB317544)

Example of human DAP12 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human DAP12 ELISA Kit (AB317544)

Interpolated concentration of native DAP12 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 60 µg/mL THP-1 cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Tissue Extracts

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 2 pg/mL

Range

15.625 - 1000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

style

left-column

Product details

Human DAP12 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of DAP12 protein in Cell Lysate, Tissue extracts. Quantitate Human DAP12 with 2 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)

-Also available in a fully validated 384-well format.

style

left-column

Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.1" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.9" } ]

style

left-column

Recovery

[ { "sample": "Cell Lysate", "range": "95 - 109 %", "average": "= 103" }, { "sample": "Tissue Extracts", "range": "92 - 106 %", "average": "= 97" } ]

style

left-column

What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab317544-10x96Tests", "assetComponentDetails": [ { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB317544-CMP09" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB317544-CMP08" }, { "size":"10 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB317544-CMP04" }, { "size":"20 x 1 Vial", "name":"Human DAP12 Lyophilized Recombinant Protein", "number":"AB317544-CMP03" }, { "size":"10 x 600 µL", "name":"10X Human DAP12 Capture Antibody", "number":"AB317544-CMP01" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB317544-CMP06" }, { "size":"2 x 50 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB317544-CMP05" }, { "size":"10 x 600 µL", "name":"10X Human DAP12 Detector Antibody", "number":"AB317544-CMP02" }, { "size":"10 x 1 Unit", "name":"Plate Seal", "number":"AB317544-CMP11" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB317544-CMP10" } ] }, "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab317544-1x384Tests", "assetComponentDetails": [ { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB317544-CMP09" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB317544-CMP08" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB317544-CMP04" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB317544-CMP06" }, { "size":"1 x 600 µL", "name":"10X Human DAP12 Capture Antibody", "number":"AB317544-CMP01" }, { "size":"2 x 1 Vial", "name":"Human DAP12 Lyophilized Recombinant Protein", "number":"AB317544-CMP03" }, { "size":"1 x 50 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB317544-CMP05" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB317544-CMP12" }, { "size":"1 x 600 µL", "name":"10X Human DAP12 Detector Antibody", "number":"AB317544-CMP02" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB317544-CMP11" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab317544-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB317544-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB317544-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB317544-CMP08", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB317544-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB317544-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human DAP12 Capture Antibody", "number":"AB317544-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human DAP12 Lyophilized Recombinant Protein", "number":"AB317544-CMP03", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB317544-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human DAP12 Detector Antibody", "number":"AB317544-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB317544-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB317544-CMP11", "productcode":"" } ] } } }

style

left-column

Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

style

left-column

style

left-column

Product protocols

style

left-column

Target data

Adapter protein which non-covalently associates with activating receptors found on the surface of a variety of immune cells to mediate signaling and cell activation following ligand binding by the receptors (PubMed : 10604985, PubMed : 9490415, PubMed : 9655483). TYROBP is tyrosine-phosphorylated in the ITAM domain following ligand binding by the associated receptors which leads to activation of additional tyrosine kinases and subsequent cell activation (PubMed : 9490415). Also has an inhibitory role in some cells (PubMed : 21727189). Non-covalently associates with activating receptors of the CD300 family to mediate cell activation (PubMed : 15557162, PubMed : 16920917, PubMed : 17928527, PubMed : 26221034). Also mediates cell activation through association with activating receptors of the CD200R family (By similarity). Required for neutrophil activation mediated by integrin (By similarity). Required for the activation of myeloid cells mediated by the CLEC5A/MDL1 receptor (PubMed : 10449773). Associates with natural killer (NK) cell receptors such as KIR2DS2 and the KLRD1/KLRC2 heterodimer to mediate NK cell activation (PubMed : 23715743, PubMed : 9490415, PubMed : 9655483). Also enhances trafficking and cell surface expression of NK cell receptors KIR2DS1, KIR2DS2 and KIR2DS4 and ensures their stability at the cell surface (PubMed : 23715743). Associates with SIRPB1 to mediate activation of myeloid cells such as monocytes and dendritic cells (PubMed : 10604985). Associates with TREM1 to mediate activation of neutrophils and monocytes (PubMed : 10799849). Associates with TREM2 on monocyte-derived dendritic cells to mediate up-regulation of chemokine receptor CCR7 and dendritic cell maturation and survival (PubMed : 11602640). Association with TREM2 mediates cytokine-induced formation of multinucleated giant cells which are formed by the fusion of macrophages (PubMed : 18957693). Stabilizes the TREM2 C-terminal fragment (TREM2-CTF) produced by TREM2 ectodomain shedding which suppresses the release of pro-inflammatory cytokines (PubMed : 25957402). In microglia, required with TREM2 for phagocytosis of apoptotic neurons (By similarity). Required with ITGAM/CD11B in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development (By similarity). Promotes pro-inflammatory responses in microglia following nerve injury which accelerates degeneration of injured neurons (By similarity). Positively regulates the expression of the IRAK3/IRAK-M kinase and IL10 production by liver dendritic cells and inhibits their T cell allostimulatory ability (By similarity). Negatively regulates B cell proliferation (PubMed : 21727189). Required for CSF1-mediated osteoclast cytoskeletal organization (By similarity). Positively regulates multinucleation during osteoclast development (By similarity).

See full target information TYROBP

style

left-column

style

left-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

style

left-column

websiteProtocolBooklet

style

right-column

style

bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com