Human DAP12 (pY91) ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the semi-quantitative measurement of Human DAP12 (pY91) Human in Cell Lysate samples.
Colorimetric
Cell Lysate
Semi-quantitative
Human
1h 30m
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Adapter protein which non-covalently associates with activating receptors found on the surface of a variety of immune cells to mediate signaling and cell activation following ligand binding by the receptors (PubMed:10604985, PubMed:9490415, PubMed:9655483). TYROBP is tyrosine-phosphorylated in the ITAM domain following ligand binding by the associated receptors which leads to activation of additional tyrosine kinases and subsequent cell activation (PubMed:9490415). Also has an inhibitory role in some cells (PubMed:21727189). Non-covalently associates with activating receptors of the CD300 family to mediate cell activation (PubMed:15557162, PubMed:16920917, PubMed:17928527, PubMed:26221034). Also mediates cell activation through association with activating receptors of the CD200R family (By similarity). Required for neutrophil activation mediated by integrin (By similarity). Required for the activation of myeloid cells mediated by the CLEC5A/MDL1 receptor (PubMed:10449773). Associates with natural killer (NK) cell receptors such as KIR2DS2 and the KLRD1/KLRC2 heterodimer to mediate NK cell activation (PubMed:23715743, PubMed:9490415, PubMed:9655483). Also enhances trafficking and cell surface expression of NK cell receptors KIR2DS1, KIR2DS2 and KIR2DS4 and ensures their stability at the cell surface (PubMed:23715743). Associates with SIRPB1 to mediate activation of myeloid cells such as monocytes and dendritic cells (PubMed:10604985). Associates with TREM1 to mediate activation of neutrophils and monocytes (PubMed:10799849). Associates with TREM2 on monocyte-derived dendritic cells to mediate up-regulation of chemokine receptor CCR7 and dendritic cell maturation and survival (PubMed:11602640). Association with TREM2 mediates cytokine-induced formation of multinucleated giant cells which are formed by the fusion of macrophages (PubMed:18957693). Stabilizes the TREM2 C-terminal fragment (TREM2-CTF) produced by TREM2 ectodomain shedding which suppresses the release of pro-inflammatory cytokines (PubMed:25957402). In microglia, required with TREM2 for phagocytosis of apoptotic neurons (By similarity). Required with ITGAM/CD11B in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development (By similarity). Promotes pro-inflammatory responses in microglia following nerve injury which accelerates degeneration of injured neurons (By similarity). Positively regulates the expression of the IRAK3/IRAK-M kinase and IL10 production by liver dendritic cells and inhibits their T cell allostimulatory ability (By similarity). Negatively regulates B cell proliferation (PubMed:21727189). Required for CSF1-mediated osteoclast cytoskeletal organization (By similarity). Positively regulates multinucleation during osteoclast development (By similarity).
DAP12, KARAP, TYROBP, TYRO protein tyrosine kinase-binding protein, DNAX-activation protein 12, Killer-activating receptor-associated protein, KAR-associated protein
Human DAP12 (pY91) ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the semi-quantitative measurement of Human DAP12 (pY91) Human in Cell Lysate samples.
Colorimetric
Cell Lysate
Semi-quantitative
Human
1h 30m
Pre-coated microplate (12 x 8 well strips)
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample THP-1 Extract | n 6 | mean - | SD - | C.V. 6.8 |
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample THP-1 Extract | n 3 | mean - | SD - | C.V. 4.3 |
Blue Ice
+4°C
+4°C
+4°C
Human DAP12 (pY91) in vitro SimpleStep ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the semi-quantitative measurement of human DAP12 (pY91) protein in human cells.
The SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or controls are added to the wells, followed by the Antibody Cocktail. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
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Cell line analysis for Human DAP12 Total from preparations of 17,500 cells/well cell extracts. Data from triplicate measurements (mean +/- SD) are plotted.
Induction of Human DAP12 (pY91) phosphorylation in THP-1 cells in response to pervanadate treatment, and levels of Human DAP12 Total in the same samples. THP-1 cells were added to 96-well tissue culture plates at 2 x 10e6 cells/well and treated (10 min) with a dose-range of pervanadate before cell lysis. Data from duplicate measurements of Human DAP12 (pY91) are plotted and compared against Human DAP12 Total protein levels.
Example of a typical Human DAP12 (pY91) control lysate dilution series in 1X Cell Extraction Buffer PTR. The DAP12 human lysate dilution series was prepared as described in Section 10. Raw data values are shown in the table.
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