Human DC-SIGNR ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human DC-SIGNR in Human in Plasma, Cell culture supernatant, Serum samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.164 - 40 ng/mL
- Sensitivity
- = 0.16 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Probable pathogen-recognition receptor involved in peripheral immune surveillance in liver. May mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. Is a receptor for ICAM3, probably by binding to mannose-like carbohydrates. (Microbial infection) Acts as an attachment receptor for Ebolavirus. (Microbial infection) Acts as an attachment receptor for Hepatitis C virus. (Microbial infection) Acts as an attachment receptor for HIV-1. (Microbial infection) Acts as an attachment receptor for Human coronavirus 229E. (Microbial infection) Acts as an attachment receptor for Human cytomegalovirus/HHV-5. (Microbial infection) Acts as an attachment receptor for Influenzavirus. (Microbial infection) Acts as an attachment receptor for SARS-CoV. (Microbial infection) Acts as an attachment receptor for West-nile virus. (Microbial infection) Acts as an attachment receptor for Japanese encephalitis virus. (Microbial infection) Acts as an attachment receptor for Marburg virus glycoprotein. (Microbial infection) Recognition of M.bovis by dendritic cells may occur partially via this molecule.
Alternative names
CD299, CD209L, CD209L1, CLEC4M, C-type lectin domain family 4 member M, CD209 antigen-like protein 1, DC-SIGN-related protein, Dendritic cell-specific ICAM-3-grabbing non-integrin 2, Liver/lymph node-specific ICAM-3-grabbing non-integrin, DC-SIGNR, DC-SIGN2, L-SIGN
What's included?
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Human DC-SIGNR ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human DC-SIGNR in Human in Plasma, Cell culture supernatant, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.164 - 40 ng/mL
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 0.16 ng/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 10 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 12 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 109.3 | Range 89 - 131 % |
Sample type Plasma | Average % = 100.5 | Range 69 - 139 % |
Sample type Cell culture media | Average % = 119 | Range 114 - 123 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Human DC-SIGNR ELISA Kit (ab277435) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human DC-SIGNR in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human DC-SIGNR coated on a 96-well plate. Standards and samples are pipetted into the wells and Human DC-SIGNR present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human DC-SIGNR antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human DC-SIGNR bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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ELISA - Human DC-SIGNR ELISA Kit (ab277435)
These standard curves are for demonstration only. A standard curve must be run with each assay.
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Product protocols
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