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AB277435

Human DC-SIGNR ELISA Kit

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Human DC-SIGNR ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human DC-SIGNR in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

CD299, CD209L, CD209L1, CLEC4M, C-type lectin domain family 4 member M, CD209 antigen-like protein 1, DC-SIGN-related protein, Dendritic cell-specific ICAM-3-grabbing non-integrin 2, Liver/lymph node-specific ICAM-3-grabbing non-integrin, DC-SIGNR, DC-SIGN2, L-SIGN

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ELISA - Human DC-SIGNR ELISA Kit (AB277435)
  • ELISA

Supplier Data

ELISA - Human DC-SIGNR ELISA Kit (AB277435)

These standard curves are for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.16 ng/mL

Range

0.164 - 40 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human DC-SIGNR ELISA Kit (ab277435) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human DC-SIGNR in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Human DC-SIGNR coated on a 96-well plate. Standards and samples are pipetted into the wells and Human DC-SIGNR present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human DC-SIGNR antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human DC-SIGNR bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Serum", "range": "89 - 131 %", "average": "= 109.3" }, { "sample": "Plasma", "range": "69 - 139 %", "average": "= 100.5" }, { "sample": "Cell culture media", "range": "114 - 123 %", "average": "= 119" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab277435-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB277435-CMP04", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB277435-CMP01", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB277435-CMP02", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB277435-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human DC-SIGNR coated Microplate", "number":"AB277435-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human DC-SIGNR Antibody", "number":"AB277435-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human DC-SIGNR standard protein (Lyophilized)", "number":"AB277435-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB277435-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Probable pathogen-recognition receptor involved in peripheral immune surveillance in liver. May mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. Is a receptor for ICAM3, probably by binding to mannose-like carbohydrates.. (Microbial infection) Acts as an attachment receptor for Ebolavirus.. (Microbial infection) Acts as an attachment receptor for Hepatitis C virus.. (Microbial infection) Acts as an attachment receptor for HIV-1.. (Microbial infection) Acts as an attachment receptor for Human coronavirus 229E.. (Microbial infection) Acts as an attachment receptor for Human cytomegalovirus/HHV-5.. (Microbial infection) Acts as an attachment receptor for Influenzavirus.. (Microbial infection) Acts as an attachment receptor for SARS-CoV.. (Microbial infection) Acts as an attachment receptor for West-nile virus.. (Microbial infection) Acts as an attachment receptor for Japanese encephalitis virus.. (Microbial infection) Acts as an attachment receptor for Marburg virus glycoprotein.. (Microbial infection) Recognition of M.bovis by dendritic cells may occur partially via this molecule.
See full target information CLEC4M
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websiteProtocolBooklet
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